Posted by admin on Jul 31, 2022 in |
Background: Pain is a common symptom having huge public health importance. Conventional analgesics relieve pain effectively but cause various unwanted side effects. Hence the search for a novel analgesic with favourable risk-benefit profile is essential. Objectives: The current study was undertaken to investigate the analgesic activity of the ethanolic, hexane and chloroform extracts of sesame seeds by thermal heat method in Albino mice. Methods: The mice were divided into 5 groups. Group 1 was administered normal saline. Group 2 was given aspirin (50 mg/kg). Groups 3 to 5 were administered the various Sesame seed extracts (ethanolic, hexane and chloroform) at the doses of 100, 150 and 200 mg/kg, respectively. All drugs were administered orally. Analgesic activity was evaluated by hot plate method. Results: The ethanolic extract of Sesame seed at the dose of 100 mg/kg and 150 mg/kg exhibited significant analgesic activity at 120 and 180 minutes, whereas at a dose of 200 mg/kg it exhibited activity even at 60 minutes. The maximum analgesic effect was shown at 120...
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Posted by admin on Jul 31, 2022 in |
Background: Sepsis leads to approximately 11 million deaths annually. Objectives: We studied the effectiveness of using a combination of intravenous hydrocortisone (50mg QID), Ascorbic Acid (1.5g QID), and Thiamine (200mg BD) (HAT) to reduce mortality among septic patients compared to a historical control group. Materials and Methods: This retrospective, observational study was conducted between January 2016 – February 2018. Our hospital mortality for septic shock is 55%. According to published literature, hospital mortality decreased from 40.4% to 8.5% with the HAT protocol. To detect a 31.9% mortality difference with 95% confidence and 80% power, a minimum sample size of 27 patients would be required in each group. We included 62 patients (31 per group) with septic shock. In the experimental group, patients in septic shock received the HAT drugs within 6 hrs and continued for 4 days. They were compared with a propensity score-matched historical control group. Results: Nineteen (61.29%) and twenty-seven (87%) patients in the control group received steroid and vitamin supplements (non-Vitamin C), respectively. The mortality was...
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Posted by admin on Jul 31, 2022 in |
Objectives: To investigate and compare the analgesic effect of fluoxetine (FLU), duloxetine (DUL) and venlafaxine (VEN) in animal models of chronic pain. Methods: Chronic pain was induced in albino rats using spared nerve injury and vincristine. Rats were divided into Control group/ Normal Saline (NS5), DUL group, FLU group, and VEN group. In spared nerve injury, sural branch of sciatic nerve was kept intact while another two branches, common peroneal and tibial nerve ligated and cut. In the vincristine-induced neuropathic pain model, vincristine was administered along with test drugs for 5 days and then 2 days gap and then for 5 days. Mechanical allodynia and hyperalgesia developed in animals were indicative of pain. Pain assessment was done by using von frey filaments by measuring paw withdrawal threshold (PWT) on day 7 in spared nerve injury and on 7 and 14 days of vincristine administration. Results: In spared nerve injury, DUL and FLU showed a significant increase in PWT compared to the control group. Venlafaxine had no analgesic action in...
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Posted by admin on Jul 31, 2022 in |
Molecular Docking is an effective and competent tool for in-silico screening. It is playing an important and ever-increasing role in rational drug design. The main application lies in this structure-based virtual screening is the identification of new active compounds towards a particular target protein. In present investigation, few N1-chloroacetyl-7-substituted- 4-methyl-1, 5-benzodiazepine-2-one and 7-Substituted-4-methyl-1,5-benzodiazepin-2-one are designed and docked at active site of cavity 1# of GABA-A receptor-associated protein (1KJT) to identify their hypothetical binding mode. As a target protein, we used the X-ray crystal structure of mammalian GABA-A receptor-associated protein from the Protein Data Bank. The Molecular Design Suite was used in this study to conduct docking investigations and conformational analyses. The comparative docking experiments of designed compounds with known GABA agonist, Clobazam was carried out. The dock scores calculated for Clobazam was -5.2598. Among the designed compounds, following conformation were found to have lower dock scores as indicated in bracket; N1-chloroacetyl-7-bromo-4-methyl-1,5-benzodiazepine-2-one, Conformer_C4 (-4.7869) and 7-chloro-4-methyl-1,5-benzodiazepin-2-one, Conformer_C13 (-5.0485) and said to have better affinity for active site of GABA-A receptor-associated...
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Posted by admin on Jul 31, 2022 in |
Worldwide antimicrobial resistance is a serious problem and differs from region to region. This study has designed the determination of the incidence and sensitivity pattern of Escherichia coli (E. coli) and discusses the general issues related to antimicrobial resistance. Prospective study was carried out between JAN to April 2020. Samples of urine, blood, pus and miscellaneous (fluids, swabs and sputum) were collected from indoor patients. The present antimicrobial susceptibility of E. coli, the study was performed in the Department of Microbiology Narayana Super-specialty Gurugram, Total of 315 samples were tested out, 97 samples showed growth on culture and out of 97 samples, 40 E. coli have isolated and tested antibiotics sensitivity, it has been demonstrated that different samples showing a significant amount of antimicrobials Sensitivity and resistance to different antibiotics (Table 2). Antimicrobials, namely Meropenem have shown sensitivity (65%), imipenem (60%), Colistin (70%). Thus, there is a need for the implementation of strong antimicrobial stewardship program and stop misuse of antibiotics with an instant effect. Our region’s present study...
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