Posted by admin on Sep 30, 2022 in |
Iron overload has toxic effects on the body. Hepatotoxicity is a common finding in iron-overloaded patients, resulting from iron deposition in hepatocytes. Angiotensinɪɪ is involved in developing hepatic damage through its pro-oxidative properties. Therefore, the current study aimed to investigate the therapeutic effect of the ACE inhibitor, lisinopril, against ferrous sulfate-induced hepatic functional disorders and histological damages in rats, due to its inhibitory effect on angiotensin II and its ability to scavenge free radicals in-vitro. 23 male adult rats were randomly classified into three groups and treated as follows: Normal control group: received daily 1 ml/day distilled water as an intraperitoneal injection for 14 days, Iron overload non-treated group: received ferrous sulfate at a daily dose of 30 mg/kg/day i.p. for 14 days, Iron overload treated with lisinopril group: received ferrous sulfate at a dose of 30 mg/kg/day followed by lisinopril at a dose of 1 mg/kg/day daily i.p. for 14 days. Compared to the control group, administration of ferrous sulfate resulted in liver dysfunction, as evidenced by significantly...
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Posted by admin on Sep 30, 2022 in |
Proximate composition, qualitative screening for phytochemicals (terpenoids, steroids, tannins, oxalates, coumarins, phenols, saponins, and flavonoids) and antioxidant properties in four selected indigenous oilseeds: morama (Tylosema esculentum), mongongo (Schinziophyton rautenenii), mogose (Bauhinia petersiana) and kgengwe (Citrulus lanatus (Thunb.) Mansf.) from Botswana was examined. A significant difference (p≤0.05) was observed in the moisture, fat, protein, ash, carbohydrate, and energy contents. The values ranged from 3.87 – 7.27, 15.99 – 57.70, 12.44 – 33.76, 3.07 – 6.07, and 6.31 – 60.01%, respectively, while the range for the energy content was from 422.35 to 655.29 kcal/100g. Only oxalate was not detected in the qualitative tests performed for eight (8) phytochemical compounds with six (6) solvent extracts. The highest total phenolic content was recorded in morama seeds (1.72 mg GAE/mL), while total flavonoids were better in mogose (0.00813 mg CE/g) and kgengwe (0.00404 mg CE/g). The vitamin C equivalent antioxidant capacity (VCEAC) for mogose, morama, and kgengwe were 2.39, 2.07, and 0.78 mg/100 g, respectively. The study indicated that the oilseeds could potentially be...
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Posted by admin on Sep 30, 2022 in |
The use of natural compounds has become more preferable for reducing the severe complications of diabetes mellitus due to having a minimum cost and the least side effects. The recent study is aimed to diagnose in-vitro α-amylase inhibition activity and the molecular interactions of various phytocompounds like momordicoside A, momordicoside B, momordicoside C, momordicoside D, momor-dicoside E, gallic acid, β-sitosterol, chlorogenic acid, vicine, gentisic acid, epicatechin, apiole, campesterol, cycloeucalenol, lophenol, cisdihydrocarveol, transnerolidol, daucosterol, arachidic acid, lauric acid, palmitic acid, momordenol, obtusifoliol, γ-linoleic acid, steric acid, oleic acid, capric acid and myristic acid present in seeds of Momordica charantia Linn. The molecular docking of the 3D structure of these compounds has been studied with target diabetic protein i.e., α-amylase. 2D interactions were also studied by using Ligand Interaction Diagram. The interaction fingerprinting was generated using the Interaction fingerprint tool available in Maestro (version 12.5) of Schrodinger Suite version 2020-3. The results demonstrated that bioactive compounds i.e. momordicoside A, momordicoside C, momordicoside D and momordicoside B, with a higher binding affinity...
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Posted by admin on Sep 30, 2022 in |
Sensitive and selective liquid chromatography/tandem mass spectrometry (LC–MS/MS) method was developed and validated for warfarin (WAR) in buffered SD (Sprague Dawley) rat plasma (PBS and Plasma: 50:50, v/v) using propranolol (PRP) as internal standards (IS) with the Plasma protein binding (PPB) assay samples to a determination of free and total warfarin. Plasma protein precipitation extraction technique with acetonitrile containing internal standard was used for extraction of the analyte. A generic gradient method with a short run time of 2.0 min was developed for the analysis of warfarin with a C18 analytical column (Synrgi 4 µm Fusion-RP, 50X2 mm), mobile phase composed of aqueous phase: 0.1% formic acid containing Milli-Q-water and Organic Phase 0.1% formic acid containing acetonitrile. Detection was performed on a triple quadrupole mass spectrometer that employed the Positive electrospray ionization (ESI) technique, operating in multiple reaction monitoring (MRM), with the transitions of m/z 309.2-163.1. The analysis was carried out over a linear concentration range of 1–1200 nM, where the 0.1% of the free fraction of the compound...
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Posted by admin on Sep 30, 2022 in |
Background: Halela (Terminalia chebula Retz) is one of the constituents in the well-known Unani compound medicine Itriphal, which has been used for thousands of years to treat a variety of ailments. In order to improve the therapeutic activity of the drug, the present study adapted the process of Tadbeer / Mudabbar (rectification/purification) which is based on the concept of Islah-e-Advia. This process thus helps in making the drug effective, safe, and specific, or minimizing undesirable characteristics by acting on the drug’s constituents without compromising its medicinal value through various methods (rectification process). Objectives: To further grasp the significance of the notion of Islah-e-Advia, before and after Halela Mudabbar (rectification), a comparative analysis was conducted. Methods: Mudabbar was done by roasting dry Halela powder with Almond oil for 7-10 minutes on a low flame on standard cooking gas. Physico-chemical and quantitative HPLC examinations of gallic acid, ellagic acid, chebulagic acid, and chebulinic acid were performed with Halela before and after the Mudabbar procedure. Results: Quantitative HPLC analysis of gallic acid,...
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