Posted by admin on May 31, 2022 in |
The present study aims to develop a verse phase HPLC method for the simultaneous estimation of Metformin and Saxagliptin. In bulk and pharmaceutical dosage form and to validate the proposed method in accordance with ICH guidelines for the intended analytical application. Multiple trials were run to Metformin and Saxagliptin, get eluted with good peak symmetric properties. Mobile phase Phosphate buffer: Methanol: Acetonitrile (40:5:55), Column Hypersil silica and flow rate 1.0ml, detection wavelength at 233nm, column temperature 300C and diluents Water: Methanol (50:50). Column Hypersil silica and flow rate 1.0 ml, detection wavelength at 210 nm conditions were finalized as an optimized method. This method was validated for Stability Data, System suitability, Precision, Linearity, Accuracy, Robustness, Ruggedness, LOD, and LOQ. System suitability parameters were studied by injecting the standard five times, and results were well under the acceptance criteria. Linearity study was carried out between 25% to 150% levels, r2 value found 0.999. By using the above method, assay of the marketed formulation was carried out 99.63% was present. So,...
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Posted by admin on May 31, 2022 in |
A novel series 3(a–d) 1-(4-benzhydryl piperazin-1-yl)-3-(di substituted) propan-1-one &1-(4-benzhydrylpiperazin-1-yl)-3-(piperidin-1-yl) propan-1-one (3e) & 1-(4-benzhydrylpiperazin-1-yl)-3-morpholinopropan-1-one (3f) compounds have been synthesized and screened for its anticonvulsant and neurotoxic activity, After i.p. injection to rat at doses of 30, 100, and 300 mg/kg body weight. Synthesized compounds were examined in the maximal electroshock-induced seizures (MES). Spectroscopic data were consistent with the structure of newly synthesized compounds. The neurotoxicity was assessed using the rotarod method. In the prepared series, 3f was found to be active in the MES screen at 0.5 h, and 4...
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Posted by admin on May 31, 2022 in |
Leucas aspera species, auspicious in many cultures worldwide, are sources of novel bioactive secondary metabolites. The mechanism by which they exhibit therapeutic potential via antioxidant, antimicrobial and apoptotic properties is scant. The main objective of the current study was to evaluate the properties of activity-guided fractionated root methanol extract. As the methanol extract exhibited the highest antioxidant activity, it was evaluated for secondary metabolites and therapeutic properties UPLC-MS analysis of the extract identified the 15 and five secondary metabolites including oleanolic acid, 6-C-glucosyl- 8- C- arabinosylapigenin, 6- C-arabinosyl 8- C-glycosylapigenin, neohesperidin and beta-sitosterol were reported. Anti-bacterial activity by disc diffusion inhibited human pathogenic bacteria. Bacterial bio-film inhibition capacity of extract (750 µg) imaged by Confocal laser scanning microscopy revealed loss of microcolonies. Further, it quenched free radicals produced by Fentons’ reagent studied by DNS-nicking assay, indicating its strong antioxidant property. The MTT assay results identified maximum cell death for human breast cancer cells, MCF-7 (EC50, 60.31±2.71 mg/mL-1). CLSM visualized a clear indication of apoptosis via chromatin condensation. The presence...
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Posted by admin on May 31, 2022 in |
Glinus oppositifolius (Linn) is a branched herb containing linear to obovate, opposite leaves with greenish flowers, and it belongs to the molluginaceae family. This bitter leafy vegetable is known as gima shak in West Bengal, Assam & Bangladesh. This leaf contains various bioactive components which provide protection against diabetes, hyperlipidaemia, joint pain, inflammation, diarrhea, skin disorder, etc. Previous nutritional analysis in our laboratory found that the iron content of these gima leaves is high. A randomized study was conducted among poor adult subjects who are socio-economically backward and have no chronic diseases. 18-65yrs aged people were randomly selected from slum areas of Kolkata. Among them, Fifty-Five samples consumed 50 gm boiled gima paste as supplementation for 45 days. Data on their dietary habits, anthropometry, medication habits, and haematological parameters were analyzed. Supplementation of gima revealed that there is70%&64% increase in haemoglobin among men and women respectively, whereas females of 40-60 years showed more positive changes than men. After supplementation of gima, the iron storage i.e. ferritin level, showed a 76% and 66.6% increase in...
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Posted by admin on May 31, 2022 in |
Background: Covid-19 has become a global threat. All most all the countries are facing this pandemic. This pandemic has greatly affected the medical education and medical students who are tomorrow’s doctors. So, they need to have a wide awareness regarding the knowledge, attitude, and practice towards this Covid-19 infection. Objective: To study the knowledge, attitude, and practice of medical students regarding the Covid–19 pandemic. Material and Method: This descriptive and cross-sectional study was conducted on 260 medical students of J.L.N. Medical College, Ajmer (Rajasthan). A total of 20 questionnaires in which, 10 in the knowledge section, 5 each in the attitude and practice section, were provided in July – August, 2021. Results: A total of 260 responses were collected and studied. The male to female ratio in the study population was 0.91. A total of 54.46% of students gave correct responses regarding the knowledge section. 87.31% of students show a positive attitude towards vaccination. Overall positivity regarding attitude was 76.61%. 95 % of Students were proactive regarding washing their...
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