Posted by admin on Sep 30, 2022 in |
Background: The present study was carried to evaluate the phytochemical, antioxidant, and anti-diabetic potential of Abies pindrow leaf extracts. Methods: The extracted leaf samples of Abies pindrow in methanolic and hydroalcoholic solutions were evaluated for phytochemical and antioxidant activities. In-vitro antioxidant activity of both test samples was calculated by DPPH radical scavenging capacity, reducing power activity, total phenolic and flavonoid contents. Further the study was carried to evaluate the anti-diabetic potential of hydroalcoholic extract of Abies pindrow leaves in Alloxan-induced diabetic rats for 21 days. Various biochemical parameters and histopathological studies were also carried out. Results: The hydroalcoholic extract of Abies pindrow was found to have more antioxidant potential than the methanolic extract. Also the same extract at high dose (400mg/kg) exhibited significant anti-diabetic activity than the low dose (200mg/kg) in alloxan-induced diabetic rats. The hydroalcoholic extract also showed improvement in parameters like body weight and lipid profile as well as regeneration of β-cells of the pancreas in alloxan-induced diabetic rats. Histopathological studies show reinforcement of the healing of...
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Posted by admin on Sep 30, 2022 in |
Objectives: Gymnosporia montana (family Celeastraceae), important shrub which is widely reported in Gujrat, India. In this study, our major objective is to screen secondary metabolites of methanolic leaves and fruit extract and determining its antioxidant and anti-inflammatory potential of this shrub. Materials and Methods: Leaves and fruit extract were subjected to methanol followed by phytochemical investigations and estimating the presence of secondary metabolites and also analysing its activity for antioxidant (DPPH and hydrogen peroxide scavenging methods) and anti-inflammatory potentiality were carried out. Results: Preliminary studies related to its phytochemical screening of leaves and fruit extract of Gymnosporia montana in the form of methanol extract which revealed its existence of flavonoids, alkaloids and terpenoids. In comparison with alkaloid and terpenoid extract, there is significant enhancement in antioxidant activity of flavonoid content in case of methanolic extract in a dose-dependent manner. Thereafter, itsanti-inflammatory activity test was carried out against Typhoid vaccine (25 µg/ml, 10 µl) with these extracts (flavonoids, terpenoids and alkaloids of methanol) using variable concentration and compared with Typhoid...
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Posted by admin on Sep 30, 2022 in |
Introduction / Objective: In this study, we aimed to elucidate the action of ultra-diluted (potency 6CH as per homeopathic pharmacopeia) ethanolic extract of Arsenicum album (Arsenic 6CH) on cytokine gene expression changes along with alteration of hepatic histology in Gallus gallus embryo (embryonated egg) challenged with the spike protein (S protein RBD of Delta SARS-CoV-2). Methods: Allantoic fluid was collected during harvesting of the inoculated eggs after antigenic challenge. The gene expression analysis of interferons (IFN α, IFN β, IFN γ); transforming growth factor-beta 1 (TGF β1); interleukins (IL-6, IL-8, IL-10, IL-1β) were studied in real-time PCR. Pre- and post-treatment sets were separately studied. Hepatic tissue of the embryos was collected for histological study. Results: The most prominent changes were found with IL-10 expression, which was significantly increased directly by Arsenic 6CH; in pre-and post-treatment experimental sets, its level was further increased, while in control sets, such changes were not delineated. Some changes were found in relation to INF β, IL-8, IL-1β, and TGF β1 in alcohol 6CH (potentized...
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Posted by admin on Sep 30, 2022 in |
Substituted quinoxaline has received considerable attention during the last few years as they are endowed with various biological activities and have a wide range of therapeutic properties. Piperazine has proven its worth in numerous clinically active drugs broader areas of therapeutic index. Hence, biologically important quinoxaline derivatives containing a piperazine moiety and Schiff base scaffolds were prepared. The structures of the synthesized compounds were characterised by elemental analysis, IR, NMR, and Mass spectra. All the prepared derivatives were screened for antibacterial and antifungal activity by disc diffusion method using nutrient agar media against Bacillus subtilis, Bacillus pumilus, Escherichia coli and Pseudomonas aeruginosa, & potato dextrose agar medium for activity against Aspergillus niger and Candida albicans using ciprofloxacin as a standard for antibacterial and clotrimazole as a standard for antifungal activity respectively. Fluoro and trimethoxy-containing derivatives have shown promising activity against gram–ve bacteria P. aeruginosa. Some compounds have shown moderate antifungal and antibacterial...
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Posted by admin on Sep 30, 2022 in |
Multiple sclerosis (MS) is a chronic demyelinating disease of the central nervous system characterized by Neuroinflammation, oligodendrocyte loss, and axonal pathology. Bergenin, a chief phytochemical constituent of Bergenia species, has been shown to exert anti-inflammatory and antioxidant effects. The cuprizone (CPZ) model is an established mouse model of MS and causes demyelination and induces motor dysfunction, cognitive impairment, and Neuroinflammation leading to damage in the myelinated neuronal region of the brain. Our study examined the potential neuroprotective effects of Bergenin in cuprizone-intoxicated C57BL/6 mice. Mice were fed with chow containing 0.2 % cuprizone for 42 days, followed by Bergenin treatment (25 mg/kg and 50 mg/kg/day, i.p) given for 42 days. At the end of the experimental period, animals were tested on behavioural activities to evaluate changes in balance and motor coordination. Mice were then sacrificed to estimate the biochemical status, and histology of brain sections was used to assess the demyelination rate and myelin inflammatory status. The aim of this study was to examine the neuroprotective effects of Bergenin...
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