Posted by admin on Apr 30, 2022 in |
Seven pathogenic isolates with clinical history were procured from the Department of Microbiology, RMMCH, Annamalai University, Chidambaram, Tamil Nadu. Among them, five isolates (S1-S5) showed multi-drug resistance to the selected antibiotics. Maximum multi-drug resistance was exhibited by four Gram negative isolates (S1, S2, S4 and S5). Initial qualitative analysis of these four isolates for biofilm forming efficacy by the Congo-Red Agar method revealed that three of these isolates (S1, S2 and S4) were biofilm producers. Maximum biofilm formation was exhibited by S4. Further qualitative analysis of biofilm formation by S4 using the Tube Adherence method yielded a positive result. Biofilm forming efficacy of S4 was then quantitatively analyzed by a spectrophotometric assay. An optical density of 0.38 was recorded at a wavelength of 540 nm, which was significantly higher than the O.D. value of the control (0.05), confirming biofilm formation by the...
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Posted by admin on Apr 30, 2022 in |
In Benin, several studies still confirm the circulation of low-quality anti-malarial drugs, despite efforts to improve the supply chain. It is, therefore, necessary to carry out post-market quality surveillance of the same to ensure that only quality and efficacious medicines are sold to the general populace. The aim of this work was to evaluate the post-market quality of fixed-dose combinations of artemether-lumefantrine marketed in Benin. This descriptive and analytical study was executed in four stages over fourteen months, from December 2019 to January 2021. Thirty samples of artemether-lumefantrine (80/480 mg) fixed-dose combination tablets were collected from sixteen pharmacies in Cotonou and Porto Novo. The various quality control activities and tests conducted were: visual identification, pharmacopoeial tests (weight uniformity and disintegration tests), identification tests, in-vitro comparative dissolution tests and active ingredients content determination by HPLC-UV. The results of our analyses showed that none of the samples passed the visual identification test; all the samples passed the weight uniformity test in accordance with the specifications of the European pharmacopeia (Eur Ph)....
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Posted by admin on Apr 30, 2022 in |
Metformin, the first-line drug, can reduce hepatic gluconeogenesis and increase insulin sensitivity. At the molecular level, metformin inhibits the mitochondrial respiratory chain, which leads to an increase in adenosine monophosphate-activated protein kinase, which increases insulin sensitivity. Recent guidelines advocate metformin as the first-line agent that has a glucose-lowering effect concurrently with lifestyle modification. Metformin has long been shown to decrease vitamin B12 levels. Vitamin B12 is also an essential micronutrient required for optimal hemopoietic, neuro-cognitive, and cardiovascular function. Vitamin B12 deficiency has been highly prevalent among patients with type 2 diabetes mellitus. In this study, we want to figure out the association between metformin administration and VitB12 absorption defects in a diabetic patient pool on metformin alone and metformin with other combination drugs. Findings from this study indicate the role of metformin in vitB12 deficiency, uncontrolled glucose levels, and levels of serum Minerals in T2DM patients, gradually leading to microvascular and macrovascular complications even with one year of usage of...
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Posted by admin on Apr 30, 2022 in |
Embelin was one of active constituents of Embelia ribes Burm. f. Embelin has been reported to possess many pharmacological effects. The studies as was perform to enhance the bioavailability of poorly water soluble embelin, reduce the dose of Embelin, prevent the degradation of Embelin in the GIT. For this, phytosome, a novel drug delivery system, was adopted. Total six Embelin loaded phytosome complex formulations coded as EMBP1, EMBP2, EMBP3, EMBP4, EMBP5 and EMBP6 using soya lecithin and Chitosan in variable ratio were prepared by anti-solvent precipitation method and Rotary evaporation technique. The phytosome was characterized by parameters like percentage yield, particle size determination, entrapment efficiency, scanning electron microscopy (SEM), in-vitro release studies and establish drug release kinetic studies. Among six formulations, the Formulation EMBP5 well optimized. EMBP5 exhibits lowest 345.45 ±1.231 diameter. The entrapment efficiency of Embelin loaded phytosome complex varies from 64.99 ± 1.546 to 81.78 ± 1.151%. Embelin loaded phytosome complex formulation (EMBP5) exhibits highest of 81.78 ± 1.151% entrapment efficiency. SEM photomicrographs of optimized formulation (EMBP5)...
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Posted by admin on Apr 30, 2022 in |
With a view to enhancing endogenous knowledge about plants, the present study has been aimed at developing and standardizing dosage forms based on the aqueous extract of Morinda lucida Benth, a plant with hypoglycaemic anti-hyperglycaemic properties. After the aqueous extraction of the leaves of this plant, by maceration, phytochemical screening was carried out on a TLC plate by using the method of Wagner and Bladt (1996) then three capsule formulations were produced (F1, F2 and F3). Different pharmacotechnical tests were conducted according to the European Pharmacopoeia (Eur. Ph.). The presence of alkaloids, flavonoids and saponosids in the aqueous extract attests to its hypoglycaemic and anti-hyperglycaemic properties. Likewise, their presence in the capsules gives them these same properties and shows the neutrality of the used excipients on the phytochemical’s compounds of the extract. The average capsule masses are 190.525 mg, 250.04 mg, and 381.54 mg, respectively, for the formulas F1, F2, and F3 capsules. The polyphenols that were used as a tracker in the aqueous extract did not show a...
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