Posted by admin on Apr 30, 2022 in |
Background and Objectives: A sper requisition of current regulatory requirements, a simple, rapid, and sensitive method by 33 factorial QbD approach was established and validated for Pioglitazone (PGZ) Glimepiride (GPR) by RP-HPLC. Method: A simple RP-HPLC method has been developed and validated with different parameters such as linearity, precision, repeatability, LOD, LOQ, accuracy as per International Conference for Harmonisation guidelines (Q2R1). Statistical data analysis was done for data obtained from different aliquots Runs on Agilent Tech. Gradient System with Autoinjector, UV (DAD) & Gradient Detector. Results: Equipped with Reverse Phase (Agilent) C18 column (4.6 mm × 100 mm; 2.5µm), a 20µl injection loop and UV730D Absorbance detector at 231nm wavelength and running chemstation 10.1 software and drugs along with degradants were separated via Methanol: (0.1% OPA) Water (70:30) of pH 3.2 as mobile phase setting flow rate 0.7 ml/min at ambient temperature. The developed method was found linear over the concentration range of 15-75 μg/ml for PGZ and 2-10 μg/ml for GPR, while detection and quantitation limit was found...
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Posted by admin on Apr 30, 2022 in |
The discovery of alternative medications in diabetes management is of huge importance to achieve better safety and efficacy. The natural antioxidant and carbohydrate hydrolyzing enzyme inhibitors are gaining too much importance in the management of diabetes. Lack of scientific data is available on antioxidant and antidiabetic activities on root extract of Spermadicyton suaveolens. Therefore, we aimed to investigate the in-vitro antioxidant and α- amylase potential of Spermadicyton suaveolens. The roots were extracted by the successive Soxhlet extraction process. The extracts were evaluated for the presence of chemical constituents. Besides, the extracts were assessed for their antioxidant potential using a DPPH assay and α- amylase assay by the DNSA method. Phytochemical analysis acknowledged the presence of secondary metabolites alkaloid, phenol, saponins, steroids respectively. All the extracts showed moderate to high antioxidant and enzyme inhibition potential. Amongst all extracts, ethanolic extract showed the highest free radical scavenging potential (IC50= 324 mg/mL). Moreover, the ethanolic extract also showed better α- amylase enzyme inhibition action (IC50= 602 mg/mL). The finding from the study...
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Posted by admin on Apr 30, 2022 in |
Medicinal plants abound with many phytochemicals that are effective in representing lots of pharmacological activities. The current study aimed at investigating the phytochemical and pharmacological activity of Ethanolic extract of Capparis moonii Wight. The analgesic activity was examined by Eddy’s hot plate method and the acetic acid-induced writhing method. In Eddy’s Hot plate method, after oral administration of EECM at doses of (50, 100, and 150 mg/kg body weight), at the end of the experiment i.e., at 120 min, the percent MPA was 137.70 ± 2.511, 166.99 ± 2.511 and 211.83±10.648% respectively; whereas the reference drug Pentazocine displayed 288.54±10.379% MPA at the dose of 10 mg/kg as compared to the control, and the results were statistically significant (p < 0.01). In the acetic acid-induced writhing test, after oral administration of EECM at doses of (50, 100, and 150 mg/kg body weight), the percent inhibition was 27.08±1.098, 47.62±4.113, and 71.75 ± 3.127%, respectively. In contrast, the reference drug Ibuprofen displayed 77.70±1.180% inhibition at the dose of 100 mg/kg as compared...
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Posted by admin on Apr 30, 2022 in |
Osteoarthritis (OA) commonly affects many joints in the human body, including the knee joints. The knee joint is made up of two parts – the patella-femoral (PF) and tibiofemoral (TF) compartment. Animal models for OA studies are well-established and serve as an important tool for understanding the impact of OA in humans. This study evaluates the anti-OA potential of a multi-herbal extract blend product – KEC (KARALLIEF EASY CLIMB) against monosodium iodoacetate induced OA in wistar rats in-vivo. Animals have equally divided into four groups, of which one group was the control, and other three groups were experimental groups. Biomarker estimation and histopathological evaluation were carried out. All values were expressed as mean ± SEM. The significant difference between the treatment and control group was estimated using one-way ANOVA with Dennett’s test. All results of the statistical analysis were summarized in separate tables. In any case, the values were considered statistically significant at P<0.05. This study found that treatment with KEC reduced the Monosodium Iodoacetate (MIA) induced osteoarthritis condition....
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Posted by admin on Apr 30, 2022 in |
Current investigation was to evaluate antidiarrhoeal effect of Casuarina equisetifolia. Anti-diarrhoeal activity of methanol bark extract of Casuarina equisetifolia (CEME) studied on experimentally induced diarrhea in rats, castor oil-induced diarrhoea & enteropooling, meal transit time, and PGE2-induced entropooling. The CEME orally administered at doses 100, 200, and 400 mg/kg/atropine 0.1 mg/kg/ received control, respectively, were administered 30 min prior to the administration of castor oil. The CEME 100, 200 and 400 mg/kg/atropine 0.1 mg/kg/ received control to rats fed with as charcoal meal. Thirty minutes later, the intestinal distances moved by the charcoal were measured. The PGE2-induced enteropooling rats were treated with CEME orally at 100, 200, and 400 mg/kg/loperamide 2.5 mg/kg/1 ml of 5% v/v ethanol in normal saline, afterward PGE2 was administered orally to each rat, and intestine fluid volume was measured. The CEME 100, 200 and 400 mg/kg produced inhibition on the frequency of defecation by 33.89%, 42.66%, 59.64 %) and in diarrhoeic drops 35.83%, 43.06%, 49.72%. In PGE2-induced entropooling, intraluminal fluid accumulation reduced significantly. The...
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