Posted by admin on Jun 30, 2022 in |
Objective: The present work aimed to prepare flurbiprofen tablets using different concentrations of super disintegrants like croscarmellose sodium, sodium starch glycolate cross povidone sublimating agent thymol. Methods: The tablets were prepared by direct compression and sublimation technique using different concentrations of super disintegrants. The prepared tablets were evaluated for different parameters like hardness, friability, weight variation, thickness, content uniformity, wetting time, disintegration time, and dissolution study. Morphology of pure drug and optimized formulation were revealed by using SEM. Results: All the formulations prepared with the highest concentration of super disintegrant showed the highest drug release. Formulations from F11 to F13 were prepared with the sublimation technique. F11 formulation showed the highest drug release because of its porous nature, which resulted from the sublimation technique. Conclusion: The optimized formulation of the sublimation method (F11) was porous in nature. In-vitro drug release of all formulations prepared with the highest concentration of super disintegrant showed higher drug release than lower concentrations. The formulations prepared by the sublimation technique showed good drug release...
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Posted by admin on Jun 30, 2022 in |
This current investigation was designed to assess in-vitro the ability of the extract to prevent free radical-induced oxidative cell damages. Fibroblasts were exposed to hydrogen peroxide and treated with gradual concentrations of ethanol and dichloromethane fruits pulp extracts from Adansonia digitata and Ziziphus mauritiana. The cell viability was measured after 24h of treatment by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium (MTT) assay. Moreover, the capacity of extracts to scavenge free radicals was evaluated by using the DPPH and ABTS models. The polyphenol contents of extracts were also quantified by spectrophotometry. All extracts in a concentration between 100 and 400 µg/mL didn’t exhibit any cytotoxic effects on fibroblasts compared to the vehicle (p >0.05). However, extracts showed interesting cytoprotective activity against the hydrogen peroxide-induced oxidative cell damage. The cytotoxic effects of the hydrogen peroxide were completely suppressed by adding the extracts of A. digitata. The ethanol extract of A. digitatas showed the best free radical quenching activities on DPPH radical (IC50 = 28.78 ± 4.97 µg/mL) and ABTS radical (IC50 =25.29 ±...
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Posted by admin on Jun 30, 2022 in |
The aim and objective of the study are to determine the nature, frequency, and potential risk factors of drug-related problems in pediatric patients. For three months, a prospective observational study was carried on 56 pediatric patients at Niloufer Hospital, India. A data collection form was prepared, and DRPs were identified based on the classification criteria of PCNE for drug-related problems (V9.1). A one-tailed Fisher’s exact test or Chi-square test was used wherever appropriate to find a significant association between potential risk factors and DRPs. Odds ratio and confidence interval of 95% were used to see the strength of association. P < 0.05 was considered to be statistically significant. SPSS version 22.0 (copyright IBM Corporation and other(s) 1989, 2013) was used for performing statistical analysis. A total of 80 DRPs were identified with 173 causes. A mean of 1.43 with a standard deviation of 0.97 DRPs per patient ranging from 0 to 4 DRPs per patient was found. Nearing half the sample size, [22 (39.29%)] had two DRPs per patient. This...
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Posted by admin on Jun 30, 2022 in |
Hepatocellular carcinoma (HCC) is liver cancer characterized by high prevalence and low survival rate. Hence, there is an urgent need to screen resources to develop therapeutic drugs to treat patients suffering from HCC. In this context, the current study involves developing a microcystin conjugated nano-drug from cyan bacteria Anabaena cylindrica to target the tumor liver cells. The microcystin molecules in the aqueous algal extract were quantified by High-performance liquid chromatography (HPLC) and used as reducing and capping agents at a concentration below the toxic limit to synthesize silver nanoparticles. These nanoparticles were characterized by Nanoparticle Tracking analysis (NTA), Fourier Transform Infra-red (FTIR), Transmission emission microscopy (TEM), and their anti-tumor activity was evaluated on HepG2 cell line by MTT assay. The highest nanoparticle synthesis was obtained at pH 8.5 after 120 min of incubation. NTA and TEM analysis showed mean particle size of 30nm. FTIR spectrum confirmed the presence of functional groups related to proteins conjugated to silver nanoparticles. The nanoparticle formulation exhibited enhanced to toxic activity in HepG2 cell...
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Posted by admin on Jun 30, 2022 in |
The present paper describes a novel, isocratic, simple, precise, accurate, and robust reversed-phase high-performance liquid chromatographic (RP-HPLC) method development and validation for simultaneous quantitative estimation of Ofloxacin and Beclomethasone dipropionate in an Ophthalmic/Otic formulation. The proposed chromatographic estimation was carried out isocratically using Protonsil C18 (250 × 4.6mm) SH 5.0µm column, the mixture of 0.02 M potassium dihydrogen phosphate buffer (pH adjusted to 3 using ortho-phosphoric acid): acetonitrile in a ratio of 30:70 v/v with a flow rate of 1 mL/min was used as mobile phase and column oven adjusted to 30°Cwith injection volume 10µL. The ultraviolet (UV) detection was carried out at 234 nm. The retention time of ofloxacin and beclomethasone dipropionate was found to be 2.67±0.2 min and 7.42±0.2 min, respectively. Calibration curves were linear over the tested concentration range of 4 to 20µg/mL. The present study reveals that this novel isocratic HPLC method is well-validated, reliable, and can be used for routine analysis of ofloxacin and beclomethasone dipropionate in an otic formulation containing these as one...
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