Posted by admin on Apr 30, 2022 in |
Osteoarthritis (OA) commonly affects many joints in the human body, including the knee joints. The knee joint is made up of two parts – the patella-femoral (PF) and tibiofemoral (TF) compartment. Animal models for OA studies are well-established and serve as an important tool for understanding the impact of OA in humans. This study evaluates the anti-OA potential of a multi-herbal extract blend product – KEC (KARALLIEF EASY CLIMB) against monosodium iodoacetate induced OA in wistar rats in-vivo. Animals have equally divided into four groups, of which one group was the control, and other three groups were experimental groups. Biomarker estimation and histopathological evaluation were carried out. All values were expressed as mean ± SEM. The significant difference between the treatment and control group was estimated using one-way ANOVA with Dennett’s test. All results of the statistical analysis were summarized in separate tables. In any case, the values were considered statistically significant at P<0.05. This study found that treatment with KEC reduced the Monosodium Iodoacetate (MIA) induced osteoarthritis condition....
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Posted by admin on Apr 30, 2022 in |
Current investigation was to evaluate antidiarrhoeal effect of Casuarina equisetifolia. Anti-diarrhoeal activity of methanol bark extract of Casuarina equisetifolia (CEME) studied on experimentally induced diarrhea in rats, castor oil-induced diarrhoea & enteropooling, meal transit time, and PGE2-induced entropooling. The CEME orally administered at doses 100, 200, and 400 mg/kg/atropine 0.1 mg/kg/ received control, respectively, were administered 30 min prior to the administration of castor oil. The CEME 100, 200 and 400 mg/kg/atropine 0.1 mg/kg/ received control to rats fed with as charcoal meal. Thirty minutes later, the intestinal distances moved by the charcoal were measured. The PGE2-induced enteropooling rats were treated with CEME orally at 100, 200, and 400 mg/kg/loperamide 2.5 mg/kg/1 ml of 5% v/v ethanol in normal saline, afterward PGE2 was administered orally to each rat, and intestine fluid volume was measured. The CEME 100, 200 and 400 mg/kg produced inhibition on the frequency of defecation by 33.89%, 42.66%, 59.64 %) and in diarrhoeic drops 35.83%, 43.06%, 49.72%. In PGE2-induced entropooling, intraluminal fluid accumulation reduced significantly. The...
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Posted by admin on Apr 30, 2022 in |
The leaves and roots extracts of Clerodendrum infortunatum was evaluated for its potential antioxidant and antibacterial properties. The correlation studies with phytochemicals were performed, which has not been widely reported yet. Clerodendrum infortunatum leaves and roots were screened for the presence of phytochemicals and were found positive for carbohydrates, saponins, alkaloids, tannins, phytosterols, coumarin glycoside, cardiac glycoside, phenols, flavonoids, and terpenoids. The phenols were quantified using the Folin-Ciocalteu method. The phenolics were found to be highest in ethyl acetate leaf (63.83 mg GAE/g) and ethyl acetate root extracts (87.21 mg GAE/g) at a concentration of 500 µl. Flavonoids were quantified by aluminium chloride colorimetric method, and it was found highest in ethyl acetate leaf extract (244.16 mg QE/g) and hexane root extracts (38.83 mg QE/g) at 500 µl. Aqueous leaf extracts (0.99 g) and hexane root extracts (0.99 g) had the highest total terpenoid content. The antioxidant activity was evaluated using DPPH assay. Aqueous leaves extract and ethyl acetate root extracts exhibited higher DPPH radical scavenging activity with an...
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Posted by admin on Apr 30, 2022 in |
Gliclazide is an anti-diabetic medication used to treat diabetes mellitus type 2.It suffers from deprived solubility, reduced drug dissolution, and bioavailability. The current paper is aimed to formulate and evaluate gliclazide solid dispersion with HP β Cyclodextrin for enhanced solubility and incorporate into controlled-release tablet formulation. Gliclazide solid dispersion (SD) was prepared using varying ratios of HP β Cyclodextrin and evaluated. The optimized SD formulation was incorporated into the tablet by using hydroxypropyl cellulose and HPMC K 100M. The formulation SD 3 was chosen based on solubility and evaluation parameters, hence formulated into a controlled release matrix tablet formulation. Around 15 formulations of controlled release tablet blends evaluated for micrometric properties demonstrate that all the formulations posses good flow properties. The controlled release tablet formulation F15 with maximum drug content of 99.99% and drug dissolution of 99.96 % over 16h was chosen optimal and characterized. In-vivo bioavailability studies of optimized formulation (F15) and marketed products are performed on rabbits. The Cmax of the optimized formulation (132.56 ± 0.08ng/ml) was...
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Posted by admin on Mar 31, 2022 in |
Remogliflozin Etabonate (RE) is the latest addition to the sodium-glucose transport proteins 2 inhibitor class of drugs recently approved in India to manage type 2 Diabetes Mellitus. Literature survey revealed that no high-performance thin-layer chromatographic (HPTLC) method had been reported to date for this drug. The present work describes the development and validation of an HPTLC method for RE. The chromatography was performed on pre-coated silica gel 60 F 254 plates using methanol: toluene: ethyl acetate (1:4:5) v/v/v as mobile phase. A thin layer chromatographic (TLC) scanner set at 228 nm was used to directly evaluate the chromatograms in reflectance/absorbance mode. The drug was satisfactorily resolved with Rf 0.45. The method was validated according to the International Council on Harmonization (ICH) guidelines. The calibration plot was linear between 50–300 ng/b and respectively. The accuracy and precision of the proposed method were evaluated by recovery studies and intra-day and inter-day precision studies, respectively. In stability testing, RE was found to be susceptible to alkaline degradation. Because the method could effectively...
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