Posted by admin on Nov 30, 2021 in |
The present work deals with the development and validation of a novel, robust, precise, and accurate UV-spectrophotometric method for the estimation of canagliflozin in bulk and formulation using the principle of Analytical Quality by Design (AQbD). A central composite design (CCD) was employed for initial parameter screening and method optimization. Different statistical parameters were evaluated to decide the appropriateness of experimental data. Canagliflozin shows an absorption maximum at 290 nm using methanol and water. Factor screening slit width and sampling interval were identified as critical method variables, which were further evaluated by a CCD. Design Expert (Version 12.0.6.0, Stat-Ease Inc., Minneapolis, MN USA) software was used for the method optimization and generation of design space Good linearity was obtained for canagliflozin in the range of 5-25 μg/mL with R2> 0.9989. The method was found to be accurate with good average % recovery. The developed method was validated as per ICH guidelines. Based on AQbD development of the Spectrophotometric method ensured that quality is built into the method. The method...
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Posted by admin on Nov 30, 2021 in |
This study was designed to evaluate the phytochemical findings for the presence of Alkaloids, Flavonoids, Phenols, and Terpenoids, and the phytochemical profile of chloroform and ethanol extracts of Panicum trypheron were analyzed using GC-MS (Gas Chromatography-Mass Spectrometry) method. The leaves were dried, powdered, and soaked in chloroform and ethanol, then subjected to GC-MS analysis by using Clarus 680 Gas chromatography. The unknown spectrum was compared with that of the known by using NIST (National Institute of Standards and Technology) database, which revealed the presence of different chemical entities in each of the extracts. The compounds are pharmacologically and biologically noteworthy. The extracts showed good antibacterial activity when tested against selected bacterial strains. This preliminary study gives an overview of the plant, which contains bioactive compounds and is therefore proposed as a pharmaceutically important...
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Posted by admin on Nov 30, 2021 in |
This study aims to valorizeten medicinal plants traditionally used for oral care in Benin by producing scientific data on their toxicities, antimicrobial activities, and chemical properties. The ten medicinal plants (Zanthoxylum zanthoxyloides, Vitellaria paradoxa, Prosopis africana, Dialium guineense, Pseudocedrela kotschyi, Parkia biglobosa, Azadirachta indica, Diospyros mespiliformis, Anogeissus leiocarpa and Bridelia ferruginea) were identified following an ethnobotanical survey conducted in Benin. With the exception of the hydroethanolic extract of Z. zanthoxyloides, all extracts have an LC50 greater than 0.1 mg/mL, indicating that these plants are non-cytotoxic. Of the ten extracts tested, only the hydroethanolic extract of Z. zanthoxyloides stem has no activity on the bacterial strains used. The results of the antibacterial test show that the largest diameter of inhibition was obtained in the extract of A. leiocarpa on Escherichia coli ATCC 25922, a reference strain with 25.67mm. The highest levels of total flavonoids (FVT) and total polypenols (TPP) are recorded respectively in P. kotschyi (145.86±0.7 EC/gMS) and D. guineense (70.194±0.220 EGA/g Ms). The antiradical powers of the extracts with...
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Posted by admin on Nov 30, 2021 in |
The study was designed to optimize cultural conditions for enhanced production of bioactive compounds by Streptomyces olivo-verticillatus VLK-15 isolated from mangrove ecosystem of South Coast of Andhra Pradesh, India. The soil dilution plate technique was employed for the isolation of actinobacteria using starch-casein agar (ISP-6) medium. The potent strain was identified as Streptomyces olivoverticillatus VLK-15 based on polyphasic taxonomy. The productivity of the strain was enhanced by amending the medium (ISP-2) with mannitol (0.5%), peptone (0.5%), and K2HPO4 (0.05%) adjusted to pH 7.0 and incubated with the culture at 30ºC for ninety-six hours. The secondary metabolites produced under optimized conditions exhibited high antimicrobial activity against Gram-positive and Gram-negative bacteria as well as fungi. Hence, it could be a possible source for novel and potential antimicrobial...
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Posted by admin on Nov 30, 2021 in |
Shyonak is botanically equated to Oroxylum indicum (L.) Kurz. of family Bignoniaceae. It is extensively used in the Indian system of medicine as an important ingredient of ‘Dashmoola and also one of the important constituents of a reputed Ayurvedic formulation ‘Chyavanprash’. The drug consists of root bark of Oroxylum indicum (L.) Kurz. Ayurvedic Pharmacopoeia of India (API) advocates that the stem bark can be used in case of the non-availability of the root bark. The root and stem bark is used for the treatments of diarrhoea, dysentery, erythema, gastralgia, hoarseness, infantile, measles, sore throat, urticaria, snake-bite and scorpion-sting. During the market survey it was found that the stembark of Ailanthus excelsa Roxb. (Family- Simarubaceae) is being sold in the name of Shyonak as an adulterant. Considering these points, an attempt has been made to identify and to establish quality standards for both root bark and stem bark of Shyonaka (Oroxylum indicum (L.) Kurz. along with stem bark of Ailanthus excelsa Roxb. HPTLC fingerprint profiles showed similar and differentiating bands....
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