Posted by admin on Oct 31, 2021 in |
L-glutaminase [EC.3.5.1.2] is an amidohydrolase that catalyzes the hydrolytic deamination of L-glutamine, resulting in the production of L-glutamic acid and ammonia. The L-glutaminase has received a significant attention due to its potential as an anticancer agent. In the present study, soil sediments were collected from the estuaries region of Parangipettai, Cuddallore District, Tamil Nadu. The isolated fungal strains were screened for L-glutaminase production, and the potent strain was characterized and identified as Aspergillus flavus JK-79 (Genebank accession number MF445235). The anti-cancer enzyme L-glutaminase production by Aspergillus flavus JK-79 was evaluated in five different production media under the submerged fermentation process. The Minimal medium showed maximal enzyme production by the marine isolate. Further, parameters such as Inoculum size, pH, temperature, and nutritional requirements such as carbon sources, nitrogen sources, and amino acids were optimized by One-factor-at-a-time (OFAT) for maximal production of L-glutaminase under submerged fermentation process. The maximum production of L-glutaminase by Aspergillus flavus JK-79 was recorded at pH 8 (790 .436U/mL), temperature 28°C with 2% inoculum size. The findings...
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Posted by admin on Oct 31, 2021 in |
Plants are a source of bioactive compounds produced by different parts of the plant, maybe bark, leaves, flowers, roots, fruits, and seeds, during metabolic activities. These secondary metabolites may have protective and disease preventive properties, hence used by Traditional practioner to treat various ailments. Most plants of the Western Ghats of India remain unknown, poorly understood or unestablished for their effects on health because of unexplored phytochemical studies or insufficient scientific data. Improvement in health is possible through the intake of phytochemical supplements, which reduce oxidative stress. In recent years research on phytochemicals and search for novel high quality, inexpensive source of medicine is increasing as these do not have harmful side effects. In the present study Nothopegia racemosa, a member of Anacardiaceae, is investigated for the presence of phytochemical compounds, which would lead to further pharmacological studies. The investigation was carried out using dried and powdered bark extract using water, hydro alcohol, chloroform, petroleum ether, and hexane as solvents. The qualitative analysis revealed the presence of tannins, alkaloids,...
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Posted by admin on Oct 31, 2021 in |
The present study aimed to evaluate the effect of crude leaf extract of Hellenia speciosa on hematological parameters of alloxan-induced diabetic rats. The study was carried out using alloxan model of diabetes for a period of 28 days and at the end of the treatment rats were sacrificed and blood samples were collected for the estimation of hematological parameters. The results showed that the white blood cell (WBC) count was significantly (p<0.05) increased while red blood cell (RBC) and hemoglobin (Hb) were significantly (p<0.05) decreased in diabetic rats compared to normal rats. Treatment with the crude leaf extract of Hellenia speciosa ameliorated the imbalances in hematological parameters caused by alloxan. Moreover, Induction of diabetes does not show any significant change in the differential count, MCV, MCH, MCHC, MPV, HCT and Platelets does not show alterations in normal and diabetic control. Therefore, the crude leaf extract of Hellenia speciosa can be considered relevant in the management of the...
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Posted by admin on Oct 31, 2021 in |
The objective of the study was to formulate and evaluate controlled release glipizide matrix tablets for the release rate, release patterns, and the mechanism involved in the release process of the drug. Formulations with hydrophobic and hydrophilic polymer in several drug-to-polymer ratios and using water-soluble and water-insoluble diluents were compressed into tablets using the direct compression method. By using an eight-station dissolution rate test apparatus, the drug release study was analyzed in phosphate buffer of pH 7.4. For the determination of the release mechanism and drug release kinetics, various mathematical/kinetic models were employed. Release was relatively faster with water-soluble diluent lactose when compared to water-insoluble diluent dicalcium phosphate (DCP) at all concentrations of Starch Acetate (SA) and Ethylene Vinyl Acetate copolymer (EVA). The difference factor f1 and similarity factor f2 between prepared formulation TSAF2 and the marketed formulation was found to be similar. Therefore matrix tablets prepared to employ starch acetate (TSAF2) are considered suitable for controlled release of Glipizide over 24 h (i.e. once-a-day...
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Posted by admin on Oct 31, 2021 in |
Antimicrobial activity of the various species of Hibiscus like Hibiscus sabdariff, Hibiscus rosa-sinensis, Hibiscus trionum, Hibiscus cannabinus, Hibiscus mutabilis against various pathogens like Staphylococcus aureus, E. coli, Bacillus subtilis, Streptococcus species, Salmonella typhimurium, Klebsiella pneumonia, Pseudomonas aeruginosa, Serratia marcescens, were studied with the help of agar well diffusion method. The solvent extracts like Methanol, Ethanol, Chloroform, Acetone, Diethyl ether of all the samples were used to measure the antimicrobial activity by using Muller-Hinton agar plates which showed the highest zone of inhibition against Staphylococcus aureus, Pseudomonas aeruginosa. In Hibiscus sabdariff, Methanol, Chloroform, and Acetone extract showed the highest zone of inhibition against Staphylococcus aureus, Pseudomonas aeruginosa and Klebsiella pneumonia. In Hibiscus rosa-sinensis, Methanol and Acetone showed the highest zone of inhibition against Streptococcus species, Pseudomonas aeruginosa and Klebsiella pneumonia. Chloroform showed the highest zone of inhibition against Streptococcus species. In Hibiscus surattensis, Chloroform showed the highest zone of inhibition against E. coli. Methanol, Chloroform, and Acetone showed the highest zone of inhibition against Serratia marcescens. All the pathogens were...
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