Posted by admin on Sep 30, 2021 in |
A selective and high throughput RP-HPLC method was developed to detect and quantify Famotidine in rat plasma using Ranitidine hydrochloride as an internal standard (IS). After liquid-liquid extraction, the analyte and the internal standard were chromatographed on Enable 18H (5µ, 250×4.6mm) C18 column using 20 µL injection volume with a run time of 15 minutes. An isocratic mobile phase consisting of phosphate buffer pH 6.8 and methanol (50:50 %v/v) was used to separate drug and internal standard. The method was validated according to USFDA guidelines over the range of 50-1200 ng/mL. The absolute recovery for analyte achieved from spiked plasma sample was consistent and reproducible. Precision and accuracy (%CV) across four validation runs (LLOQ, LQC, MQC and HQC) was less than 15. LLOQ was found to be 50 ng/mL. The validated RP-HPLC method was used successfully to study Famotidine SNEDDS pharmacokinetics in rats and also quantitative determination in Solid Self Nano Emulsifying Drug Delivery System...
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Posted by admin on Sep 30, 2021 in |
Sustained released dosage form having an advantage over immediately release drug delivery system by continuous release of medicaments for a specific period in a prolonged manner. Diabetes mellitus (DM) is a common test endocrine issue that influences in excess of 100 million individuals ls around the world. Type II diabetes is characterized by peripheral insulin resistance and impaired insulin secretion. Metform in hydrochloride (Met) used in the treatment of type 2 diabetes, having a short half-life is a challenge for the researcher towards achieving sustained drug delivery systems. Met with higher dose shows high solubility and inherent compressibility. Towards the same, Met was formulated with different combinations of polymers such as chitosan, HPMCK 100 M and eudragit RL-100 by the wet granulation method. The formulated granules were subjected for a pre-compression study like bulk density, tapped density, compressibility index, Hausner’ sratio and angle of repose. The formulated tablets possess for post-compression studies. Drug-polymer interactions were investigated by FTIR studies. In-vitro drug release kinetics is carried out at pH 6.8....
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Posted by admin on Sep 30, 2021 in |
The main aim of the present study is to formulate and evaluate solid lipid nanoparticles of Metoprolol. Metoprolol is an anti-hypertension drug used in the treatment of hypertension, angina pectoris, and heart failure. Metoprolol belongs to BCS class-I, which has high solubility and high permeability. The drug needs to be administered frequently (2-3 times a day), and it has side effects and poor bioavailability of 40-50% drug through oral administration. In order to avoid the side effects and to improve its bioavailability and sustained release of the drug, there is a need to develop Metoprolol-loaded solid lipid nanoparticles. In the present study, solid lipid nanoparticles of Metoprolol were prepared by the Hot Homogenization technique. In this solid lipid nanoparticles of Metoprolol, employing Compritol as the carriers and Tween 80, PEG-400 and soya lecithin as surfactant systems and were evaluated for various parameters like entrapment efficiency, drug content and in-vitro drug release, particle size and zeta potential. The C8(1:3) formulation showed better results entrapment efficiency of 98.20%, drug content of...
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Posted by admin on Sep 30, 2021 in |
The genus Scutellaria has many species. But only a few species are investigated for the treatment of many ailments. Despite the extensive use of traditional medicine against several diseases, scientific studies of Scutellaria discolor are less. The present study is taken up to investigate the analgesic and anti-inflammatory actions of the acetone extract of S. discolour (SDA)using tail flick method with Analgesiometer and carrageenan induced hind paw edema model respectively using albino rats. The study of acute toxicity of the extract is done so as to determine the dose of the extract and to save the experimental animals. Pethidine and aspirin were used as the standard drugs for the analgesic and anti-inflammatory experiment, respectively. In tail-flick test, SDA induced a significant increase (p<0.05, p<0.001) in the reaction time to pain stimulation compared to the control showing its analgesic activity which was comparable to that of pethidine. In carrageenan-induced paw edema test, SDA showed significantly lower (p< 0.001) mean paw volume compared to the control group. But percentage inhibition of...
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Posted by admin on Sep 30, 2021 in |
The present study was to evaluate Hepatoprotective activity of aqueous extract of Hyptis suaveolens whole plant (AEHS) against Sodium arsenite (2.5 mg/kg) induced hepatotoxicity in Wistar rats for a period of 28 days. Rats were divided into five groups, each with 6 rats. Except for group I, all remaining groups was served with Sodium arsenite oral administration to induce hepatotoxicity prior to standard and plant extract administration. Group II served as sodium arsenite treated group (toxin), group III served with Vitamin-E, standard (Hepatoprotective drug) Group IV and Group V Experimental rats received AEHS orally at the doses of 250 mg/kg and 500 mg/kg daily for 28 days. Hepatoprotective effects were investigated by assessment of serum biochemical enzymes such as alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP) total bilirubin (TB), malondialdehyde (MDA), and antioxidant enzymes (SOD, CAT, GPx and GSH), along with histopathological studies. There is a significant decrease in the levels of AST, ALT, ALP and TB and an increase in the Catalase, GSH, GR and GPx...
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