Posted by admin on Oct 31, 2021 in |
In this study, a new simple, rapid and accurate, sensitive isocratic method was developed for the simultaneous determination of the Anti-retroviral drugs in pharmaceutical dosage forms. The chromatography was run through Denali C18 column (150 x 4.6 mm and 5 µm particle size). Mobile phase containing mixer of buffer 0.1% OPA (PH.4.6) acetonitrile is taken in the ratio 40:60 was pumped through column at a flow rate of 0.8ml/min and isocratic elution with a total runtime of 7 minutes. The mixer of the buffer used in this method was 0.1% OPA. The temperature was maintained at 20 °C to 30 °C, optimised wavelength selected was 258 nm. The retention time of the lamivudine and Efavirenz were found to be 2.131 min and 3.058 min as respectively. The drug was stressed under alkaline (acid, base); oxidative, thermal, photolytic degradation was analysed. The validated method was developed as per ICH Guidelines. The accuracy, linearity, precision, robustness, LOD and LOQ was within the acceptable limits. Hence, this RP-HPLC method was stability-indicating can...
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Posted by admin on Oct 31, 2021 in |
Diabetes mellitus is one of the familiar metabolic disorders affecting the global population. In the modern system of medicine, there are still no satisfactory promising agents available for the effective management and treatment of diabetes. The current study aimed to investigate the in-vitro antioxidant and antidiabetic potential of the combination of s-allyl cysteine (a phytochemical) and taurine (an endogenous biomolecule). Despite numerous pharmacotherapeutic properties of s-allyl cysteine (SAC) and taurine (TAU), their combined in-vitro effects as antidiabetic and antioxidant had not been explored, and scientific data were also lacking to be exposed as yet. The combination (SAC/TAU) gives in-vitro antioxidant activity when subjected to the tests like reducing power, hydrogen peroxide & DPPH radical scavenging assays and in-vitro antidiabetic activity when subjected to the tests like α-glucosidase & α-amylase inhibitory activities. The IC50 value were reducing power test (significant with the ascorbic acid), hydrogen peroxide radical scavenging test (4.84 µg/ml), DPPH assay (16.30 µg/ml), α-amylase inhibitory activity test (17.26 µg/ml), and α-glucosidase inhibitory activity test (5.73 µg/ml). Hence, the...
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Posted by admin on Oct 31, 2021 in |
Background and purpose of the study: Propranolol hydro-chloride beta-adrenergic receptor antagonist utilized in the treatment of high blood pressure, atrial fibrillation, myocardial infarction, angina and migraine headaches. The pharmacokinetic parameters make Propranolol hydrochloride an appropriate candidate for oral disintegrating tablets. This work aims to develop orally disintegrating tablets for Propranolol hydrochloride and to evaluate their pre-compression, physicochemical properties and water absorption ratio, disintegrating time, wetting time, in-vitro dispersion, time, and in-vitro dissolution. Research rationale: To attain rapid disintegration, dissolution/absorption, and further improving the bioavailability of the drug. To resolve swallowing issues in geriatric, pediatric patients by rapid disintegration in saliva and to treat high blood pressure, angina, atrial fibrillation, myocardial infarction, migraine. Methods: Oral disintegrating tablets prepared by direct compression technique using super disintegrants like Crospovidone, Croscarmellose sodium, Sodium starch glycolate, and Pregelatinised starch in several concentrations. The prepared batches of tablets were evaluated for pre-compression parameters and weight variation, thickness, hardness, friability, drug content, wetting time, disintegrating time, in-vitro dispersion time and in-vitro dissolution. The physicochemical interaction between...
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Posted by admin on Oct 31, 2021 in |
A simple, sensitive and rapid chromatographic method was developed and validated for quantification of Mitomycin and Fluorouracil in bulk and pharmaceutical dosage form using Symmetry C18 (4.6 × 150 mm, 3.5 µ) column. The mobile phase consists of buffer, 0.1% Ortho Phosphoric acid (OPA), and acetonitrile in the ratio of 50:50 v/v. The flow rate is maintained as at 1.0 ml/min; detection was carried out by absorption at 260 nm using photodiode array detector. The calibration curve was linear and the regression coefficient (R2) value was found to be 0.999 and concentrations ranging from 10-150 µg/ml of mitomycin and 5-75 µg/ml of fluorouracil, respectively. The LOD and LOQ of the method were found to be 0.1 µg/ml, 0.05 µg/ml and 1 µg/ml, 0.5 µg/ml for Mitomycin and Fluorouracil. The number of theoretical plates and tailing factors for Mitomycin and Fluorouracil were not less than 2000 and were not more than 2, respectively. The developed method was found to be simple, economical, suitable and validated according to ICH...
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Posted by admin on Oct 31, 2021 in |
Citrus fruit is a very rich source of Vitamin C, A, E, and flavonoids, alkaloids, and minerals. Citrus fruits are medicinal plants that present rutaceae family and include some fruits such as orange (Citrus sinensis), kinnow (Citrus reticulata), mosambi (Citrua ltmetta), grapefruit (Citrus maxima) lime, pomelo lemon. Antibacterial antiviral, antifungal activity of different solvent extracts (Acetone, ethanol, methanol, and distilled water) prepared by soxhlet extractor and aqueous solvent from three citrus fruit peel orange, kinnow, mosambi (Citrus sinensis, Citrus reticulata, and Citrus limetta respectively) were screened against four pathogenic bacteria staphylococcus aureus, Bacillus subtilis, Escherichia coli and Salmonella typhi. The highest antibacterial activity was exhibited by the acetone peel and ethanol extract of Citrus reticulate peels. MICs were tested at concentrations ranging from 80-5.25 mg/mL as wells as their MBCs. The phytochemical analysis of the citrus peel extracts showed the presence of flavonoids, saponins, steroids, terpenoids, tannins and...
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