Posted by admin on Nov 30, 2021 in |
The study was designed to optimize cultural conditions for enhanced production of bioactive compounds by Streptomyces olivo-verticillatus VLK-15 isolated from mangrove ecosystem of South Coast of Andhra Pradesh, India. The soil dilution plate technique was employed for the isolation of actinobacteria using starch-casein agar (ISP-6) medium. The potent strain was identified as Streptomyces olivoverticillatus VLK-15 based on polyphasic taxonomy. The productivity of the strain was enhanced by amending the medium (ISP-2) with mannitol (0.5%), peptone (0.5%), and K2HPO4 (0.05%) adjusted to pH 7.0 and incubated with the culture at 30ºC for ninety-six hours. The secondary metabolites produced under optimized conditions exhibited high antimicrobial activity against Gram-positive and Gram-negative bacteria as well as fungi. Hence, it could be a possible source for novel and potential antimicrobial...
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Posted by admin on Nov 30, 2021 in |
Shyonak is botanically equated to Oroxylum indicum (L.) Kurz. of family Bignoniaceae. It is extensively used in the Indian system of medicine as an important ingredient of ‘Dashmoola and also one of the important constituents of a reputed Ayurvedic formulation ‘Chyavanprash’. The drug consists of root bark of Oroxylum indicum (L.) Kurz. Ayurvedic Pharmacopoeia of India (API) advocates that the stem bark can be used in case of the non-availability of the root bark. The root and stem bark is used for the treatments of diarrhoea, dysentery, erythema, gastralgia, hoarseness, infantile, measles, sore throat, urticaria, snake-bite and scorpion-sting. During the market survey it was found that the stembark of Ailanthus excelsa Roxb. (Family- Simarubaceae) is being sold in the name of Shyonak as an adulterant. Considering these points, an attempt has been made to identify and to establish quality standards for both root bark and stem bark of Shyonaka (Oroxylum indicum (L.) Kurz. along with stem bark of Ailanthus excelsa Roxb. HPTLC fingerprint profiles showed similar and differentiating bands....
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Posted by admin on Nov 30, 2021 in |
In an attempt of synthesizing some novel and potent antioxidant activity having compounds, here some novel quinoline derivatives are reported. Initially using Vilsemeir-Hack reagent method 7-methyl or 8-methyl substituted 2-cholro-3-formylquinolines (Ia, b) were prepared. Further 7-methyl or 8-methyl substituted 2-hydroxy quinoline-3-carbaldehyde (IIa, b) were synthesized by the reaction of compound (I) on microwave irradiation with 4M HCl, which on further treatment with different substituted hydrazides yielded the novel Schiff bases of quinoline III (a-f). Purity of the synthesized compounds was determined by TLC and melting point. The structure of all newly synthesized compounds was confirmed by spectral studies such as IR, 1H NMR, 13C NMR, and mass spectroscopy. All the synthesized compounds were screened for in-vitro antioxidant activity by free radical scavenging activity by DPPH assays method and ferric ion reduction method using ascorbic acid as the standard drug. Screening results showed that all compounds are exhibiting moderate to good activity. Especially compounds III b, III d, and III f showed significant antioxidant...
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Posted by admin on Nov 30, 2021 in |
The Screening of medicinal plants for the new bioactive compounds leads to the development of new anti-microbial drugs that possess lesser cost and greater safety and efficacy. The increasing bacterial resistance exhibited by microorganisms causing skin infections has led to the research on medicinal plants. Argemone Mexicana Linn is a weed that shows multiple health benefits. All the parts of the plant are traditionally well known for the treatment of skin diseases. The aim of present study is to investigate and compare the anti-microbial activity of aqueous and ethanol extract of leaves of Argemone mexicana Linn against skin infection-causing bacteria. Also, Functional groups associated with phytochemical compounds of these plant extracts are well understood from FTIR Spectroscopy. The Argemone mexicana Linn leaves extract shows significant anti-microbial activity against all the selected microorganisms for study. The results for anti-microbial activity were recorded in zones of inhibitions. The highest zone of inhibition had shown by the ethanol extract at higher concentration, i.e., 300 mg/ml against all pathogens. The MIC values of...
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Posted by admin on Nov 30, 2021 in |
Three analytical methods were developed for the determination of selected fluoroquinolones in chicken meat by high performance liquid chromatographic technique. In each method, we took two fluoroquinolones (Danofloxacin and difloxacin; Ciprofloxacin and enrofloxacin; Ofloxacin and pefloxacin) research study. Rapid, simple and sensitive sample treatment procedures were developed and used to extract the fluoroquinolones from the chicken tissues with good recoveries. Extraction recovery from chicken tissues was in the range of 86.96 to 97.49 %, depending upon the analytes. The shape of the curves for all drugs was linear in the investigation concentration range 10-1000 ng/ml, and the correlation coefficient of the linearity curves for all drugs was in the range of 0.9707- 0.9991. The detection limits for fluoroquinolones in chicken tissues were well below the Maximum Residual Limits (MRLs) for antibiotics. The limit of detection (LOD) was in the range of 9-12 µg/kg, and the limit of quantification (LOQ) was in the range of 30-40 µg/kg for all analytes in chicken tissues. The separation results and parameters of the...
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