Posted by admin on Nov 30, 2021 in |
This study aimed to determine for taste masking & design of Oro dispersible tablet of an anticholinesterase agent (Donepezil HCl) to achieve the patient compliance. Oral dispersible tablet prepared by compression method. The Anticholinergic activity assure by evaluation, drug dissolution, stability testing and standardize the oral dispersible tablet. On the basis of the preformulating and formulation study, the batch 10-F009 formulation would maintain stability and potency throughout the study period. Complete drug release was observed at gastric pH. Taste masking and rapid disintegration of tablets formulated in this investigation may possibly help in the administration of Donepezil HCL in a more palatable form without water. Finally, this formulation with once-daily dose is convenient for patients with the Alzheimer’s disease and related dementia with good therapeutic activity and...
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Posted by admin on Nov 30, 2021 in |
Rabies is a fatal viral disease which is caused by negative sense, single-stranded RNA virus belonging to Rhabdoviridae family of genus Lyssavirus, Rabies Virus (RABV). It is transmitted by the bite of a rabid animal and directly affects the CNS of the host (neurotropic). Sources of human rabies deaths are dogs (99%), cats, bats, etc. Rabies holds the seventh position in the world for causing the most severe infections that result in the death of an organism. India lies on the top in the number of affected individuals with rabies. Our aim in this study is to analyze the interactions between the RABV and its human host and to develop novel therapeutic strategies to combat this deadly disease. Further analysis of the predicted protein-protein interactions can help us design strategies against clinical manifestations of the disease in humans. An in-silico approach has been adopted because computational approaches play a vital role in providing an important tool in further investigation of host-pathogen...
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Posted by admin on Nov 30, 2021 in |
The present study evaluated the antimicrobial and cytotoxic activities of chloroform, hexane methanol, and petroleum ether extracts of Plumbago zeylanica (PEC, PEH, PEM, and PEP). Antimicrobial activities of the extracts were evaluated against gram-positive bacteria (Staphylococcus aureus-ATCC 6538 and Bacillus cereus-NCIM 2155) and gram-negative bacteria (E. coli-ATCC 8739) along with cytotoxicity activity against cervix carcinoma cell lines (HeLa and SiHa cell lines). Out of the four extracts tested, PEM and PEC extracts showed significantly high value of zone of inhibition against tested bacteria. PEM showed zone of inhibition of 10 mm, 13mm and 8 mm in ATCC 6538, NCIM 2155 and ATCC 8739, respectively. On the other hand, chloroform extract showed a zone of inhibition of 8mm, 10mm and 7 mm in ATCC 6538, NCIM 2155 and ATCC 8739, respectively. The four extracts PEC, PEH, PEM, and PEP showed a significant cytotoxicity activity with IC50 concentration at 238.93ug/ml, 694.44ug/ml, 580.40ug/ml and 593.04ug/ml against the HeLa Cell line and 142.71ug/ml, 547.16ug/ml, 448.04ug/ml and 424.27ug/ml respectively against the SiHa Cell line...
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Posted by admin on Nov 30, 2021 in |
Cryptococcus neoformans and Candida albicans are the major causative agents for infections, cryptococcosis, and candidiasis in human. Anti-fungal drugs for the treatment of fungal infections may show less effective activity on the pathogens due to the poor absorption or inadequate drug distribution, and some pathogens may evolve anti-fungal drug resistance. The development of anti-fungal drugs from natural sources with potential activity is of main global concern. Some filamentous fungi themselves can secrete anti-fungal compounds, which show effective antagonism against numerous pathogens. One such fungus, Aspergillus giganteus is known to produce anti-fungal proteins (AFP). In this study, the AFP is purified and its antagonistic activity was determined by analyzing its pathogenic membrane disruption ability. Extracellular pH and the lipid level in the AFP treated pathogenic cell membrane were also evaluated. The results have proved the membrane damage and disintegration of the pathogenic cell membrane by AFP and were observed in a dose-time-dependent manner. The fluctuation in the extracellular pH was monitored in the treated pathogens. This study has identified a...
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Posted by admin on Nov 30, 2021 in |
Olmesartan Medoxomil and Cilnidipine combination lowers blood pressure effectively. Olmesartan Medoxomil is an angiotensin receptor blocker (ARB), and Cilnidipine is a calcium channel blocker (CCB). Cilnidipine and Olmesartan Medoxomil are widely used in hypertension. The scope of the study is to optimize the chromatographic conditions to develop a new RP-UPLC method for the simultaneous estimation of Olmesartan Medoxomil and Clinidipine, which is simple, accurate, precise, and rapid. A very few methods were developed by UPLC using DAD. The mobile phase used is 0.5% w/v Ammonium acetate buffer: methanol: Acetonitrile (40:50:10), and the detection was carried out at 240 nm by using PDA detector. The flow rate was optimized at 0.4ml/min. The retention time was found to be 0.587 and 0.992 for Olmesartan Medoxomil and Cilnidipine, respectively. All the parameters of the method development and validation meet the ICH guidelines criteria. Thus the work establishes that the reported method is more economical and can be regularly used in practical application for simultaneous analysis of the Cilnidipine (CIL) and Olmesartan medoxomil...
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