Posted by admin on Nov 30, 2021 in |
The efficacy of alcoholic Cassia fistula leaves extract on the tegument of Fasciola gigantic was studied by light microscopy. Live liver fluke Fasciola gigantica parasites were collected from the infected liver and bile duct of freshly slaughtered domestic goat (Capra hircus) at the local zoo abattoir in Udaipur. Liver flukes F. gigantica were maintained in 0.9% physiological saline and divided into three groups. The first group of the parasite was untreated used as control liver fluke, the Second group of parasites was treated with synthetic drug albendazole and the third group of parasites was given in-vitro treatments with alcoholic extracts of C. fistula leaves. The control and in-vitro treated F. gigantica with the alcoholic C. fistula leaves extract, and flukicide drug albendazole were fixed in Bouin’s fixative for histological study. The present studies revealed that the alcoholic extract of C. fistula leaves was an effect on the tegument of parasite; showed abounded cuticular disruption, detachments, discontinuous, babblings, damage in tegument and in parenchymal cell and the complete breakdown of...
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Posted by admin on Nov 30, 2021 in |
In present study, Himalayan weed Sonchus oleraceus extracts are screened for the occurrence of bioactive compounds, anti-microbial activity, and its molecular characterization using RAPD. The agar well diffusion method was used to detect anti-microbial activity of aqueous, methanol, chloroform and ethyl acetate extracts of S. oleraceus. The anti-microbial activity of different extract was checked at different concentrations i.e. at 150mg/ml, 200mg/ml, 250mg/ml and 300mg/ml against five bacteria namely Klebsiella pneumoniae, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Salmonella typhi. The anti-microbial activity of the different extracts of S. oleraceus was associated with the concentration. In phytochemical screening alkaloids, flavonoids, protein, carbohydrates, tannins, phenol, terpenoids and saponins were confirm in different extracts. The primers OPA-1, OPA-9, OPA-16, TC-1 and TC-3 were used in molecular characterization using RAPD against 14 samples collected from five districts namely Kangra, Una, Bilaspur, Hamirpur and Solan of Himachal Pradesh. Cluster analysis revealed that isolates H1 and H3, H2 and S2, U1 and U2 were showing maximum homology. In comparison to all the isolates, isolate K1...
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Posted by admin on Nov 30, 2021 in |
The goal of the research was to explain as a cognition enhancer the traditional claim of Madhuca longifolia (Sapotaceae) leaves. Dementia was activated by intracerebrovascular colchicine administration for 28 days. Madhuca longifolia leaves ethanolic extract was evaluated in mice for cognition-enhancing activity in colchicine-induced dementia using elevated plus maze (EPM), actophotometer, and biochemical estimation of brain AChE and MDA levels. Piracetam (200 mg/kg p.o.) was used as standard medicine. Transfer latency (TL) was observed in the colchicine-induced mice using EPM, showing a significant increase (p<0.01) in TL compared to the regular control group. No major effect was found on the locomotor activity of the mice. In colchicine-induced mice, a significant increase (p<0.01) in AChE and a decrease in MDA levels relative to normal control were observed. These results formed the standard argument, and Madhuca longifolia can therefore be a memory enhancer because of the presence of...
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Posted by admin on Oct 31, 2021 in |
Esmolol Hydrochloride belongs to the class II anti-arrhythmic drugs. Due to composite compositions and low reproducibility, challenges are still warranted for the systematic method development for related substances and identification of its acid degradation products. Development, validation and characterization of RP-HPLC stability-indicating method for the quantitative analysis of two impurities in Esmolol hydrochloride and its stress degradants. The validation of this method was achieved as per ICH Q2 (R1) guidelines with the optimized experimental conditions. To achieve the proposed method on C18 (250 mm × 4.6 mm, 5µ) column and the temperature was sustained at 30°C and run time was 12 min. The mobile phase consists of A–Acetonitrile, B–0.01N Potassium dihydrogen orthophosphate in water (pH=4.8). The injection volume of samples was 10μL, and UV detection was carried out at 221nm. Linearity ranges were covered 0.25-1.5 ppm for ASL-8123, ACC-9675 and Esmolol Hydrochloride. The newly developed method for separation of impurities along with the pure drugs was found to be capable of giving swift retention times while still preserving good...
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Posted by admin on Oct 31, 2021 in |
A reverse-phase liquid chromatography (LC) method was developed for the simultaneous estimation of enrofloxacin and its active metabolite ciprofloxacin residues in chicken meat. These two fluoroquinolones were extracted from homogenized tissues with phosphate buffer under acidic conditions, followed by acetonitrile. The isocratic LC analysis was performed on Phenonmenax, C18 RP column (250 × 4.6 mm, 5 μ) using a mobile phase composed of 0.025M potassium phosphate solution (pH 3.25) and acetonitrile in the ratio of 75:25 (v/v), at a flow rate of 0.500 ml/ min. Quantitation of enrofloxacin and its metabolite were performed using a PDA detector set at the wavelength of 277 nm. The temperature of the column was 300C, and the total run time was 20 min for the analysis of both drugs. The retention time was found to be 10 min for ciprofloxacin and 13 min for enrofloxacin. The r2 values for linearity studies were greater than 0.98, and the accuracy (% recovery) was greater than 88% for both the drugs. The linearity was observed in...
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