Posted by admin on Oct 31, 2021 in |
By HPTLC method, a mobile phase system comprising of Ethyl acetate: Toluene: Methanol (5:4:1v/v/v) was selected, and the detection was carried out at 280nm. The Rf values were found to be 0.65 ± 0.03 and 0.27 ±0.03 for Mebendazole and Atorvastatin, respectively, which showed a good separation. The method was validated as per ICH guidelines. The percentage RSD values of repeatability, intraday, and interday were found to be less than 2 prove the precision of the method. The correlation coefficient value from the calibration graph was found to be 0.9949 for Mebendazole and 0.9979 for Atorvastatin. The percentage protein binding of Mebendazole and Atorvastatin was estimated by the equilibrium dialysis method. The in-vitro displacement interaction study was carried out using standard solutions of a mixture containing 1.5×10-4 M Mebendazole and Atorvastatin along with 1.5×10-4 M BSA. The percentage protein binding of individual Mebendazole and Atorvastatin after 6hr was found to be 86.14% and 91.18% respectively. While they were evaluated in the presence of each other, the percentage protein binding...
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Posted by admin on Oct 31, 2021 in |
The main objective of the study was to formulate and evaluate solid self nano emulsifying drug delivery systems (SNEDDS) of famotidine (BCS class-IV drug), a histamine receptor antagonist used in case of ulcers, Zollinger-ellison syndrome. Liquid SNEDDS was formulated using oils like oleic acid, arachis oil, surfactants like Tween 20, Tween 80, etc., and co-surfactants like Transcutol-P, polyethylene glycol. Based on solubility studies, oleic acid (15mg/ml), Tween 20 (38.41mg/ml) & Transcutol-P (2.593mg/ml) were further studied by phase titration method using different ratios of oil: s-mix (surfactant: co-surfactant) from 1:9 to 9:1 with pseudo-ternary phase diagrams. FTIR spectra indicated drug excipient compatibility. Further evaluation studies, namely thermodynamic stability studies, robustness to dilution, self-emulsification time, dispersibility test, drug content, and in-vitro drug dissolution tests, formed the basis for optimization of liquid SNEDDS formulation. In-vitro drug release of F1 (97.15±0.02% in 90 min) and F19 (100.23±0.1% in 60 min) was significantly higher when compared to the pure drug (18.41±0.01% in 90 min). The droplet size of F19 (ratio of 3 (oil): 1...
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Posted by admin on Oct 31, 2021 in |
In the present study, an effort has been made to estimate Rasagiline and Riluzole simultaneously in their bulk and physical mixture by UV-Visible spectrophotometric methods. The methods developed are precise, reliable simultaneous equations and area under the curve (AUC) method. The absorption maxima in the simultaneous equation method are 265.20 nm and 263.40 nm, respectively, for rasagiline and riluzole. Similarly, in the area under the curve method, the wavelength selected for estimation is in the range of 260.20 – 270.20 nm and 258.40-268.40 nm, respectively for rasagiline and riluzole. In the two methods developed, the Beer- Lambartz’s range was in the concentration range of 50 to 250 µg/mL for rasagiline and 4 to 20µg/mL for riluzole. The percentage recovery was found to be in the range of 100.43% and 100.01% for rasagiline and riluzole. ICH guidelines were followed in the proposed methods for their reproducibility and accuracy. The proposed methods can be adopted industrially and in research laboratories for the routine simultaneous analysis of Rasagaline and Riluzole in their...
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Posted by admin on Oct 31, 2021 in |
In this study, a new simple, rapid and accurate, sensitive isocratic method was developed for the simultaneous determination of the Anti-retroviral drugs in pharmaceutical dosage forms. The chromatography was run through Denali C18 column (150 x 4.6 mm and 5 µm particle size). Mobile phase containing mixer of buffer 0.1% OPA (PH.4.6) acetonitrile is taken in the ratio 40:60 was pumped through column at a flow rate of 0.8ml/min and isocratic elution with a total runtime of 7 minutes. The mixer of the buffer used in this method was 0.1% OPA. The temperature was maintained at 20 °C to 30 °C, optimised wavelength selected was 258 nm. The retention time of the lamivudine and Efavirenz were found to be 2.131 min and 3.058 min as respectively. The drug was stressed under alkaline (acid, base); oxidative, thermal, photolytic degradation was analysed. The validated method was developed as per ICH Guidelines. The accuracy, linearity, precision, robustness, LOD and LOQ was within the acceptable limits. Hence, this RP-HPLC method was stability-indicating can...
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Posted by admin on Oct 31, 2021 in |
Diabetes mellitus is one of the familiar metabolic disorders affecting the global population. In the modern system of medicine, there are still no satisfactory promising agents available for the effective management and treatment of diabetes. The current study aimed to investigate the in-vitro antioxidant and antidiabetic potential of the combination of s-allyl cysteine (a phytochemical) and taurine (an endogenous biomolecule). Despite numerous pharmacotherapeutic properties of s-allyl cysteine (SAC) and taurine (TAU), their combined in-vitro effects as antidiabetic and antioxidant had not been explored, and scientific data were also lacking to be exposed as yet. The combination (SAC/TAU) gives in-vitro antioxidant activity when subjected to the tests like reducing power, hydrogen peroxide & DPPH radical scavenging assays and in-vitro antidiabetic activity when subjected to the tests like α-glucosidase & α-amylase inhibitory activities. The IC50 value were reducing power test (significant with the ascorbic acid), hydrogen peroxide radical scavenging test (4.84 µg/ml), DPPH assay (16.30 µg/ml), α-amylase inhibitory activity test (17.26 µg/ml), and α-glucosidase inhibitory activity test (5.73 µg/ml). Hence, the...
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