Posted by admin on Oct 31, 2021 in |
The objective of the study was to formulate and evaluate controlled release glipizide matrix tablets for the release rate, release patterns, and the mechanism involved in the release process of the drug. Formulations with hydrophobic and hydrophilic polymer in several drug-to-polymer ratios and using water-soluble and water-insoluble diluents were compressed into tablets using the direct compression method. By using an eight-station dissolution rate test apparatus, the drug release study was analyzed in phosphate buffer of pH 7.4. For the determination of the release mechanism and drug release kinetics, various mathematical/kinetic models were employed. Release was relatively faster with water-soluble diluent lactose when compared to water-insoluble diluent dicalcium phosphate (DCP) at all concentrations of Starch Acetate (SA) and Ethylene Vinyl Acetate copolymer (EVA). The difference factor f1 and similarity factor f2 between prepared formulation TSAF2 and the marketed formulation was found to be similar. Therefore matrix tablets prepared to employ starch acetate (TSAF2) are considered suitable for controlled release of Glipizide over 24 h (i.e. once-a-day...
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Posted by admin on Oct 31, 2021 in |
Antimicrobial activity of the various species of Hibiscus like Hibiscus sabdariff, Hibiscus rosa-sinensis, Hibiscus trionum, Hibiscus cannabinus, Hibiscus mutabilis against various pathogens like Staphylococcus aureus, E. coli, Bacillus subtilis, Streptococcus species, Salmonella typhimurium, Klebsiella pneumonia, Pseudomonas aeruginosa, Serratia marcescens, were studied with the help of agar well diffusion method. The solvent extracts like Methanol, Ethanol, Chloroform, Acetone, Diethyl ether of all the samples were used to measure the antimicrobial activity by using Muller-Hinton agar plates which showed the highest zone of inhibition against Staphylococcus aureus, Pseudomonas aeruginosa. In Hibiscus sabdariff, Methanol, Chloroform, and Acetone extract showed the highest zone of inhibition against Staphylococcus aureus, Pseudomonas aeruginosa and Klebsiella pneumonia. In Hibiscus rosa-sinensis, Methanol and Acetone showed the highest zone of inhibition against Streptococcus species, Pseudomonas aeruginosa and Klebsiella pneumonia. Chloroform showed the highest zone of inhibition against Streptococcus species. In Hibiscus surattensis, Chloroform showed the highest zone of inhibition against E. coli. Methanol, Chloroform, and Acetone showed the highest zone of inhibition against Serratia marcescens. All the pathogens were...
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Posted by admin on Oct 31, 2021 in |
Introduction: Drug-resistant tuberculosis (DR-TB) emerged from the time anti-tuberculosis drugs were discovered and used. It has become a formidable obstacle in the eradication of tuberculosis. India is in second position after China with tuberculosis, where about 20% of patients with DR-TB were not treated due to inappropriate coordination and diagnostic facilities. Method and Materials: A retrospective observational study aimed to assess various resistance patterns, distribution among genders, and efficacy of DR-TB regimen (2016-2019). Institutional ethical clearance with approval from the state and district TB center were obtained, and a structured data collection form was created to collect patient details registered under Ernakulam district TB unit, Kerala, India. Data obtained were statistically analyzed with Mantel-Cox test using SPSS version 25. Results and Discussion: Study shows that rifampicin resistant tuberculosis (RR-TB) is most common form of DR-TB in newly diagnosed patients. In previously treated patients, both Multidrug Resistance and RR-TB were recorded as 38%. Rifampicin and isoniazid were drugs with high resistance and on comparing the efficacy, shorter regimen had higher...
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Posted by admin on Oct 31, 2021 in |
Purpose: Lifestyle modification is recommended as a primary strategy for the management of non-alcoholic fatty liver disease (NAFLD) and non-alcoholic steatohepatitis (NASH). Recently, metabolic regulations of diets containing parts of Abelmoschus esculentus (okra) were investigated using the NAFLD mouse model. Methods: Five groups of C57BL/6J mice (n = 6) were arranged. Each received a distinct diet for 12 weeks as follows: Control group (mice fed with control diet), HFD group (mice fed with high-fat diet (HFD)), POSITIVE group (mice fed with 20 mg/kg/day simvastatin-mixed HFD), HFD-OP group (mice fed with 800 mg/kg/day okra peel powder-mixed HFD), and HFD-OS group (mice fed with 400 mg/kg/day okra seed powder-mixed HFD). Feed intake and body weight were recorded daily. After killing, blood samples were collected for measuring glucose and lipids concentrations. Visceral fat content and liver histochemistry were examined. Results: NAFLD/NASH mouse model was successfully developed by the 12-weeks HFD consumption. Increases of body weight, visceral fat, and blood glucose were not different among these five groups. HFD and Positive mice greatly...
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Posted by admin on Oct 31, 2021 in |
A simple, precise, accurate, and reliable high-performance thin-layer chromatographic method has been developed and validated for the analysis of flupirtine maleate in capsule dosage form. Identification and analysis were performed on 10 cm × 10 cm layer thickness 0.2 mm, precoated silica gel G60-F254 aluminum sheet, prewashed with methanol, and dried in an oven at 50 °C for 5 min. Ethyl acetate: Chloroform (60 : 40 ) (v/v) was used as mobile phase. A calibration plot was established, showing the dependence of response (peak area) on the amount chromatographed. The validated calibration range was found to be 50-400 ng/spot and for flupirtine maleate with correlation coefficient (r2) 0.999. The average % recovery was between 99.85-100.25%. The limit of detection and limit of quantification for flupirtine maleate were found to be 7.17 and 21.7 ng/spot, respectively. The spots were scanned with a densitometer at λ max 326nm in a reflectance mode. In this method, the drug was achieved Rf value of 0.46±0.02. The proposed method was validated as per ICH...
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