Posted by admin on Jul 31, 2021 in |
The comparative study of in-vitro seed germination was conducted to determine the influence of different concentrations of chemically synthesized copper nanoparticles (CuNPs) on the in-vitro seed germination and growth of two different varieties of wheat- PBW677 and PBW725 on MS medium. For all growth parameters taken under the present study, PBW 725 variety of wheat performed better in the basal MS media having different concentration of CuNPs than PBW 677. 100% germination percentage of PBW 725 variety of wheat is achieved on Basal MS medium supplemented with 5 ppm CuNPs. This variety has better seed vigour and tolerance index. It indicates that a lower concentration of CuNPs is potential enhancer of seed germination of this particular variety. But the higher concentration of the nanoparticles proved to be detrimental and resulted into poor seed germination and decreased morphological development of the plants of both...
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Posted by admin on Jul 31, 2021 in |
Objectives: The objective of the study is to find out the alpha-amylase inhibitory activity of Katankateryadi Kwatha. The alpha-amylase enzyme is the key source for postprandial hyperglycaemia, which is responsible for Type 2 Diabetes. Methodology: The crude drugs of Katankateryadi Kwatha (K. Kwatha) were collected from a local market. The Kwatha was prepared and dried with rotatory evaporator, under reduced pressure. Different concentrations (10, 20, 40, 60, 80 and 100 μg/ml.) of Kwatha were prepared and used for α-amylase inhibitory assay using starch as a substrate. Similarly, the protocol performed with Acarbose as standard. The absorbance was read at 595 nm using spectrophotometer. The percentage of α-amylase inhibitory activity and IC50 values of each concentration was calculated. Results & Discussion: The study shows that the Katankateryadi Kwatha has alpha-amylase enzyme inhibitory property. Standard Acarbose shows IC50 value of 38.46±0.956 with % inhibition ranged from 23% to 80% at concentration 10, 20, 40, 60, 80 and 100 µg/ml. while the inhibitory concentration (IC50 value) of K. Kwatha was found to...
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Posted by admin on Jul 31, 2021 in |
High-Performance Liquid Chromatography is an important and powerful analytical technique for the identification, quantification and isolation of natural products. In the present investigation, a Reversed-Phase- HPLC method was developed for the identification and quantification of the marker compound quercetin in Euphorbia thymifolia Linn. (whole plant) and Manilkara hexandra Roxb. (leaves). Both the plants have been used to cure various ailments since a long time in India. The results confirmed the presence of quercetin in both the plants, and its concentration was found higher in M. hexandra than E. thymifolia extract. Thus, the study justified the traditional use of these plants as medicines, and both the plants could be used in the formulation of some quality drugs in the future; however, further studies are required in this...
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Posted by admin on Jul 31, 2021 in |
Integration of nano-science in medicine leads to the development of biomedical products that help society in a faster and safer manner. In the present studies, Zinc nanoparticles were synthesized by green synthesis route using aqueous extracts of Banium persicum, and characterization was done through UV, SEM, TEM, FT-IR, and XRD. The synthesized nanoparticle was spherical in shape with an average size of 20 nm. Various bioactive compounds present in aqueous extract of this plant were responsible for bioreduction of nanoparticles. Further, these nanoparticles were tested for various biological activities. In antimicrobial activity maximum, anti-bacterial activity was observed against S. aureus at 120 µg/ml (IZ-18 mm),while in the case of fungus, maximum potential was observed at against C. albigans at 120 µg/ml (IZ- 30 mm). Further nanoparticles were screened for their antioxidant activity by LPO, Peroxidase, and Catalase assay and found they possessed potent antioxidant activity. The platelet aggregation of nanoparticles was assayed by Prothrombin (PT) and Activated Partial Thromboplastin time (APTT). In PT assay, maximum activity was observed at...
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Posted by admin on Jul 31, 2021 in |
The plant Scoparia dulcis Linn. belongs to the family Scrophulariaceae, is a native of tropical America, commonly called a sweet broom. The traditional healers identified its uses as anti-diabetic, antipyretic, antihypertensive, diuretic, and they have developed many promising traditional medicines and formulations from this plant. The main objective of this present study reveals about the phytoconstituents present in the leaves extract by preliminary phytochemical study, isolation of active compounds by column chromatography, and characterize the isolated compound by spectral studies like UV, IR, NMR, and MASS spectra. The preliminary phytochemical study reveals that the plant possesses more phytochemicals such as carbohydrates, alkaloids, tannins, phenols, flavonoids, vitamin C, and amino acids. The quantitative estimation shows that it consist of a considerable quantity of flavonoids, Phenols, Vitamin C. The methanol extract was prepared and applied in column chromatography to isolate the phytoconstituents. A dull white crystalline compound was isolated in the fraction of Acetone: Ethanol (60:40), (40:60) ratio. This compound was subjected to spectral studies. The spectral studies indicate the presence...
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