Posted by admin on May 31, 2021 in |
Breast cancer is the most common type of cancer found in women. Although considerable advances have been achieved in the diagnosis and treatment of breast cancer, medical research routinely thrives towards the development of newer technologies for the treatment of breast cancer. The current study aimed at the development of nanomedicine, in the form of silver nanoparticles from the aqueous extract of algae Spirulina platensis, for the treatment of breast cancer. The synthesis of silver nanoparticles was exhibited by UV-visible spectroscopy, and the nanoparticles were characterized by NTA, FTIR and TEM. The anti-tumor potential of this nanoparticle formulation was evaluated on MCF7 breast cancer cell line and its cytotoxicity was compared with crude aqueous algal extract as well as the recommended breast cancer chemotherapy drugs. The results obtained were promising. NTA analysis indicated a mean particle size of 72 nm. FTIR spectrum showed the presence of alcohol, phenols, carboxyl, and aromatic groups in the nanoparticle formulation, which were responsible for the biological activity. The anti-cancer activity studies revealed that...
Read More
Posted by admin on May 31, 2021 in |
Oxidative stress is a condition that might predispose individuals to diseases, including cancer. The 8- hydroxydeoxyguanosine (8-OHdG) is an index that reflects DNA damage caused by oxidative stress in the body. In this study, Commiphora molmol (Myrrh) (C. molmol) (Myrrh) that belongs to the family Burseraceae were investigated for its potential favorable properties to blunt DNA damage induced by oxidative stress employing 8-OHdG assay using human lymphocyte cultures. Lymphocytes were separated from blood samples collected from healthy volunteers, cultures, and incubated in aqueous extract of C. molmol (Myrrh) at 0, 10, 100, and 1000 µg/ml. Extracts at 10,100 and 1000µg/mL from the oleo-gum resin of C. molmol (Myrrh) significantly decreased levels of 8-OHdG. The oleo-gum resin of C. molmol (Myrrh) medicinal plants can be used as useful agents to counteract oxidative DNA damage in cultured cells. Collectively, the data revealed that oleo-gum resin of C. molmol (Myrrh) possesses favorable antioxidants and therefore lends support to its therapeutic...
Read More
Posted by admin on May 31, 2021 in |
A short selective, precise, accurate, and sensitive method for the estimation of Tolcapone and Quinapril was done by RP-HPLC. The assay of Tolcapone and Quinapril was performed with tablets, and the % assay was found to be 100.19 and 100.45, which shows that the method is useful for routine analysis. The linearity of Tolcapone and Quinapril was found to be linear with a correlation coefficient of 0.999 and 0.999, which shows that the method is capable of producing good sensitivity. The acceptance criteria of precision percent RSD should be not more than 2.0%, and the method show precision 0.2 and 0.7 for Tolcapone and Quinapril, which shows that the method is precise. The acceptance criteria of intermediate precision are RSD should be not more than 2.0%, and the method show precision 0.3 and 0.7 for Tolcapone and Quinapril, which shows that the method is repeatable when performed in different days also. The LOD and LOQ for Tolcapone were found to be 3.07 and 9.98, and LOD and LOQ for...
Read More
Posted by admin on May 31, 2021 in |
Our present study aims at analyzing and comparing the antioxidant, anti-inflammatory and phytochemical property of betel leaves from two different domains of Karnataka that includes the Western Ghats and the Northern region. Initially, the ethanol extracts of all four samples (two from each domain) were prepared and checked for their anti-inflammatory and antioxidant activity. As a result, the betel leaf extract from the western region showed good antioxidant activity and a significant anti-inflammatory activity when compared with the standard Gallic acid. In connection to this, the phytochemical content such as Alkaloids, Tannins, Flavonoids, and Phenols showed positive in all four extracts, but the quantification studies showed that the extracts from the Western Ghats had a higher quantity of alkaloids and phenols when compared to the samples from northern regions. These results imply that the presence of functionally important groups depends on the composition of the soil in which they grow in. Hence having good biologically important activity in them can prove beneficial for their role towards formulating a cost-effective...
Read More
Posted by admin on May 31, 2021 in |
Now a day’s interest towards natural and has been growing due to the unhealthy consequences of chemicals in the health industry; though, herbal substances possess several quality control and confirm pharmacological action issues. This present study was designed to determine the effects of Salix alba L. methanolic extract (MESAL) for its antioxidant and anti-inflammatory activity in rat models. Further to establish and validate a sensitive, fast and reproducible high performance thin layer chromatographic (HPTLC) method of two biomarker compounds Salicin and Ferulic acid from MESAL. The anti-inflammatory activity was studied by the carrageenan-induced rat paw oedema method while DPPH free radical scavenging ability was utilized to determine the antioxidant activity. Additionally, the separation was performed by HPTLC with quantification of markers (Salicin and Ferulic acid). Among the different combinations of mobile phases used, the best separation was achieved in Toluene: Ethyl acetate: Methanol: Formic Acid (5:3:1:1v/v/v/v). The MESAL exhibited antioxidant activity with a maximal inhibitory concentration (IC50) value of 400 µg/ml, and exerted anti-inflammatory activity, wherein 70 % protection...
Read More
