Posted by admin on May 31, 2021 in |
The objective of the research work was to design NLCs gel of Acitretin and perform an in-vitro evaluation of different formulations. FTIR and DSC studies revealed that there was no interaction between drug and polymer and with the respective surfactant. A new generation of NLCs consisting of a lipid matrix with a special nanostructure has been developed. These nanostructures improve drug loading and firmly incorporates the drug during storage. The purpose of this study was to prepare Acitretin-loaded NLCs gel effective for topical delivery of Acitretin by using the Hot Homogenization method. Stearic acid as solid lipid, Oleic acid as liquid lipid combination of two types of surfactant: Tween 80 and Sodium lauryl sulphate (SLS) were used to stabilize NLCs dispersion. Physiochemical properties of NLCs gel such as particle size, TEM, entrapment efficiency, and in-vitro release studies were investigated. The formulation in which a combination of ionic and non-ionic surfactant was used to demonstrated good entrapment efficiency, sustained in-vitro release profile. In-vitro release study revealed the biphasic release pattern...
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Posted by admin on May 31, 2021 in |
Ganoderma lucidum total triterpenes were evaluated for anti-inflammatory activity using carrageenan-induced acute and formalin-induced chronic paw oedema models in Swiss albino mice. The total triterpenes, at concentrations 10, 50, and 100 mg/kg b. wt. administered orally, showed 36.96%, 54.35%, 76.09% inhibition of acute and 41.86%, 62.79% and 79.07% inhibition of chronic inflammation. Total triterpenes were also assessed for its effect in attenuating the inflammation and modulating the antioxidant status of Freund’s complete adjuvant (FCA) induced arthritis rats. Total triterpenes when administrated orally, at concentrations 10, 50, and 100mg/kg b. wt., showed 58.73%, 73.02%, and 79.37% inhibition of paw oedema in the arthritis rats. Treatment with total triterpenes enhanced the activities of the antioxidant enzymes (SOD, GPx, and catalase) and restored the level of GSH in arthritic animals. Total triterpene treatment also attenuated the enhanced lipid peroxide levels in FCA-induced rats. All these findings support the strong therapeutic potential of Ganoderma total triterpenes against inflammation...
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Posted by admin on May 31, 2021 in |
Apremilast, the new PDE4 inhibitor drug, exhibits low solubility and low permeability; therefore, shows variable bioavailability. Effervescent floating tablets of apremilast were developed to sustain the drug release rate by increasing gastric retention time. These tablets were prepared by wet granulation technique, using different polymers like carbopol 934P, hydroxypropyl methylcellulose K4M, tragacanth and sodium alginate, either alone or in combination. Prepared tablets were characterized for physical parameters like floating capacity, hardness, % weight variation, friability, and content uniformity. Additionally, tablets were evaluated for in vitro release characteristics for 24 h. Results of this study exhibited good physical properties within the limit of acceptance. Batches prepared by carbo-pol 934P did not show any initial burst release as compared to other polymers. When polymer concentrations were increased drug release rate was decreased. Drug release from the tablets was followed by zero-order controlled release. Sodium alginate/ tragacanth and HPMC K4 in 1:3 ratio exhibited best drug release for 24 h. Carbopol batches (1:3 ratio ) showed very first floating lag time (52...
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Posted by admin on May 31, 2021 in |
In the present study, we evaluated the Anti-parkinson’s activity of Cyamopsis tetragonoloba methanol plant pod extract by Haloperidol Induced Catalepsy and Tacrine induced vacuous jaw movements for behavioral parameters and in-vivo assays to estimate neurochemical parameters like (GSH, LPO, and SOD). The behavioral analysis performed using Haloperidol Induced Catalepsy produces Parkinson’s disease (PD) like symptoms, has been shown to reduce the catalepsy significantly (P<0.001) with Cyamopsis tetragonoloba methanol plant pod extract (200 and 400 mg/kg) doses. Tacrine-induced jaw movements and bursts symptoms closely resembling the PD symptoms shown to reduce treatment with plant extract and helped to manage the uncontrolled movements. The biochemical parameters like Lipid peroxidation (LPO), Glutathione (GSH), and Superoxide dismutase (SOD) were assessed using control, standard, and 200 and 400 mg/kg Cyamopsis tetragonoloba extract, results revealed that rats treated with plant extracts had increased levels of GSH and SOD which was found to be lower in the patients of PD and further plant extract analysis found efficient in reducing the LPO activity which helps to decrease...
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Posted by admin on May 31, 2021 in |
Covid-19 pandemic has enforced the entire scientific community to work together and find a solution for the adversity the whole world is facing. This has called for immediate actions, and the most common point of discussion and rapid way to tackle this is to repurpose the previously approved molecules and check their activity against this virus. The role of computational techniques has paved the way for rapid screening of molecules so as to provide us an insight on to designing drugs to inhibit this virus. Our group has screened the Dug bank database containing 8696 molecules. These molecules were screened using three tired molecular docking protocol. We utilized 5R82 as our target structure for the main protease enzyme of SARS-CoV-2, as it was the best available structure in Protein Data Bank. After screening the database, we obtained 200 molecules having docking scores better than the standard molecules (Ritonavir and Lopinavir). Eventually, after detailed analysis, we selected three molecules DB02307, DB04226, and DB01713, for Molecular dynamics simulation study and also...
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