Posted by admin on Jul 31, 2021 in |
Hybrid molecules, furnished by combining coumarin and pyrazoles, have been synthesized in the present study. In addition, the binding ability of these coumarin-based molecules has been investigated for Nickel and less studied lanthanum. Lanthanum and nickel complexes with 4-hydroxy-3-((4-hydroxy-2-oxo-2H-chromen-3-yl)(3-(4-nitrophenyl)-1-phenyl-1H-pyrazol-4-yl)methyl)-2H-chromen-2-one (4a) and 4-hydroxy-3-((4-hydroxy-2-oxo-2H-chromen-3-yl)(1-phenyl-3-p-tolyl-1H-pyrazol-4-yl)methyl)-2H-chromen-2-one (4b) were synthesized using lanthanum nitrate hexahydrate (La(NO3).6H2O) and nickel dichloride hexahydrate (NiCl2.6H2O). The synthesized complexes 5a-d were isolated and characterized by IR, Mass, and 1HNMR spectroscopy. The spectra of complexes 5a-d were interpreted on the basis of comparison with the spectrum of the free ligand. These complexes were screened for DNA photocleavage activity. Two complexes, 5a, and 5b, gave significantly good results found to degrade both forms of DNA (SC and NC). Our data give the reason to conclude that these compounds can act as lead compounds and should be submitted to further more detailed biological...
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Posted by admin on Jul 31, 2021 in |
An accurate, precise, and simple stability-indicating and RP-HPLC method was developed and validated for the estimation of Remogliflozin Etabonate in bulk and pharmaceutical dosage forms. Primacel C18 (150 × 4.6mm, 5µm) column, with mobile phase Acetonitrile: Water (70:30, v/v) at isocratic mode, was used for the development of this method. At wavelength 280nm, and flow rate of 1ml/min was maintained. The retention time for remogliflozin etabonate was about 2.6 min. This method was validated with respect to ICH guidelines for linearity, the limit of detection, the limit of quantification, precision, accuracy, robustness, solution stability, and forced degradation studies. Linearity was performed in the concentration range of 25µg/ml to 150µg/ml with a correlation coefficient of 0.999. The percentage recovery for Remogliflozin Etabonate was found to be within limits of 98% – 102%. The %RSD was also found to be less than 2% which is within limits. Forced degradation studies result in maximum degradation occurred in alkali, acid, and peroxide degradation studies. There was no degradation occurred in photolytic and thermal...
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Posted by admin on Jul 31, 2021 in |
Nanoparticles are being constantly exploited by the scientific community due to their versatile properties and applications. However, the synthesis of metallic nanoparticles achieved through conventional physical and chemical techniques is quite expensive and a labor-intensive process with serious consequences like instability of nanoparticles and toxic byproducts. Hence, green synthesis utilizing biological systems, including plants, bacteria, yeast, fungi, and algae proves to be a cost-effective and eco-friendly alternative. Plant extracts are a rich source of many bioactive compounds like secondary metabolites and often act as reducing and capping agents in the synthesis of nanoparticles. The present work deals with the synthesis of silver nanoparticles using aqueous leaf extract of Sauropus androgynous. Prior to the synthesis, phytochemical analysis and antioxidant potential were evaluated. Physical parameters, including silver metal ion concentration and the temperature were optimized for the effective synthesis of silver nanoparticles. The synthesized nanoparticles were characterized via UV-Visible spectroscopy, Fourier transform infrared spectroscopy (FTIR), Scanning Electron Microscopy (SEM), and X-ray crystallography (XRD). Finally, silver nanoparticles were evaluated for their antibacterial...
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Posted by admin on Jul 31, 2021 in |
Antioxidants from plant sources have gained acclaim as an excellent source for living a healthy life in recent times. The present study investigates comparative quantitative characterization of phytochemicals, in-vitro antioxidant and anti-hemolytic activities among aqueous and ethanolic extracts of four Indian medicinal plants viz. Berberis aristata, Cedrus deodara, Vitex negundo and Tinospora cordifolia. Quantitative estimation of phenolics, flavonoids, and alkaloids was done by colorimetric estimation. In-vitro antioxidant capacities were evaluated by hydrogen peroxide radical scavenging assay and ferric reducing antioxidant potential assay, while total antioxidant capacity was evaluated through phosphomolybdate assay. Anti-haemolytic activity of plant extracts was assessed using H2O2 induced hemolysis of human RBCs. The aqueous extract of V. negundo and ethanolic extract of C. deodar resulted in significantly higher phenolic content amongst all plant extracts. The greatest flavonoid content was observed with an aqueous extract of V. negundo, and ethanolic extract of C. deodara demonstrated the highest alkaloid content. High antioxidant capacities were reported for the ethanolic extract of V. negundo and C. deodara. The ethanolic extract...
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Posted by admin on Jul 31, 2021 in |
Background: Cancer is continuing to be a major health problem in developing as well as undeveloped countries. It is a leading cause of mortality worldwide, accounting for most of the deaths. Epidermal growth factor receptor (EGFR) is a transmembrane glycoprotein, and mutations that lead to EGFR overexpression or overactivity have been associated with a variety of human cancers. Purpose: In-silico design of EGFR inhibitors by inhibiting EGFR kinase activity which competes with its cognate ligands, and it may potentially constitute a new class of effective drugs in cancer therapy. Experimental work: In the present investigation, we have performed a similarity/sub-structure-based search of eMolecule database and find out promising benzothiazole derivatives as EGFR inhibitors by several screening criteria, including molecular docking and pharmacokinetics features. Result and Discussion: Molecular docking clearly showed that the final proposed derivatives potential to form a number of binding interactions. Molecular dynamics simulation studies showed that the EGFR protein becomes stable when proposed derivatives bind to the receptor cavity. Strong binding affinity was found for all...
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