Posted by admin on Apr 30, 2021 in |
The prevalence of cancer is drastically increasing among cardamom farmers using chemical pesticides in Idukki district. The chemicals, mainly Organochlorine and organophosphates, result in various disease conditions. The main aim of the study was to explore the prevalence of cancer and other disease conditions among farmers and other people who were exposed to chemical pesticides. A community-based epidemiological study was conducted among cardamom farmers and people exposed to the chemical pesticides by using a pre-structured questionnaire containing socio-demographic details, information regarding chemicals, and their disease status. Descriptive and analytical statistics were applied for data analysis. A total of 237 members had interviewed. Among them, 47 were cancer patients, and 169 had comorbidities and other disease conditions. About 54 were suspected of cancer as per symptoms. Among cancers, breast cancer (36.17%) was detected as the major type, and the majority were females (23.3%). The majority of the patients 26 (28.3%) were worked for more than 20 years in the field. The majority of patients with cancer (22.3%) were not taken...
Read More
Posted by admin on Apr 30, 2021 in |
Purslane (Portulaca oleracea L.) is an herbaceous weed belonging to the family Portulacaceae. It has high nutritional value and possesses potent pharmacological actions such as analgesic, hepatoprotective, anti-inflammatory, wound healing, bronchodilator, neuropharmacological, anti-diabetic, antioxidant and antihypertensive. It is listed in a World Health Organization database because of these many health benefits. From the phytochemical investigations it is revealed that Purslane has the presence of antioxidants (vitamins A and C, α- tocopherol, β-carotene, and glutathione), linolenic acid and omega-3 fatty acids. This study aimed was to identify the linolenic acid content of Purslane extracts using HPTLC. In this study, three different solvents (Petroleum ether, methanol, and water) were used for the extraction of dried leaves and were compared. Successive solvent extraction was carried out with increasing polarity of the solvents. The extracts were analyzed for the identification of linolenic acid, and its concentration was determined using HPTLC. For HPTLC analysis, the mobile phase comprising of chloroform: methanol: water: ammonia (65:24:4:0.4 v/v/v/v) was used and the Rf value for standard linolenic acid...
Read More
Posted by admin on Mar 31, 2021 in |
The aim of this investigation was to re-evaluate the in-vitro mutagenic, genotoxic, and aneugenic potential of omeprazole through the Ames test, comet (SCGE), and cytokinesis-block micronucleus assays (CBMN), as previous reports on the mutagenic and genotoxic effects of omeprazole are scarce and inconclusive. The results of this study indicated a negative mutagenic effect through the Ames test. Comet assay endpoints such as tail length, tail intensity, and tail moment showed a mild genotoxic effect with dose-dependence. The CBMN assay endpoints were implemented according to the requirements for in-vitro evaluation of genotoxicity after 4 h of exposure, with or without metabolic activation. Endpoints, such as binucleated cells with micronuclei (BNMN), total micronuclei (MN), arrested metaphasis (AM), nuclear buds (NB), nucleoplasmic bridges (NPB), and necrotic cells (NC), showed a dose-dependent effect. The number of apoptotic cells (AP) showed a statistically significant increase compare to the control value but was without dose-dependence. This in-vitro study suggests omeprazole exhibits mild genotoxic and aneugenic...
Read More
Posted by admin on Mar 31, 2021 in |
Three simple and economical UV-spectrophotometric methods have been developed and validated for simultaneous estimation of ciprofloxacin (CIP) and metronidazole (MET) in a tablet dosage form using distilled water as a green solvent. The proposed methods were; simultaneous equation method (method A), Q-absorbance ratio method (method B), and area under curve method (method C). λmax of CIP & MET in distilled water were found to be 271 nm and 320 nm, respectively. The isoabsorptive point was observed at 290 nm. The linearity was obtained in the concentration range of 1-9 μg/ml, and 2-18 μg/ml for CIP and MET respectively by methods A, B & C. Validation parameters were carried out. All three methods were found to be linear, accurate, precise, and specific. Good results were achieved using distilled water as solvent due to its greater solubility, reproducible readings with maximum absorbance. Among the three methods, method C was found to be the most sensitive. Hence, this method can be recommended for the routine analysis of this drug...
Read More
Posted by admin on Mar 31, 2021 in |
Hypertension is now defined on the basis of systolic (SBP) and diastolic (DBP) blood pressure levels and classified into stages on the basis of the degree of elevation. The generally recognized cut point for hypertension is an average office BP of 140/90 mmHg or greater, which has been obtained by a recommended standard technique with an accurate manometer and has been confirmed on at least one other occasion. Hypertension is the main cause of high blood pressure (BP), and BP is a major public health issue in India and is rampant among both urban and rural populations. It is the key modifiable risk factor for many diseases. Prescription-event monitoring or PEM involves a methodology in which the safety of new medicines are studied that are used by general practitioners (GPs). PEM is an observational cohort form of pharmacovigilance that is non-interventional, in the sense that nothing happens to interfere with the doctor’s decision regarding which drug to prescribe for each individual patient. In this study, we focused on the...
Read More
