Posted by admin on May 31, 2021 in |
The potential of transferosomal formulation for transdermal delivery of Ginger constituents was investigated. The aim was to reduce the dosage and improve patient acceptance. Transferosomes were formulated by ethanol injection technique using Phospholipids (Lipoid P-100), cholesterol, various edge activators (tween 80, Span 80), and other excipients. Transdermal patches were prepared by solvent casting method using polymers (Ethylcellulose, HPMC), penetration enhancers (Oleic acid, Tween 80) and PEG 400 as a plasticizer. Transferosomes were evaluated for in-vitro release, particle size, zeta potential, entrapment efficiency, and stability. Patches were evaluated for folding endurance, uniformity of weight, drug content, and thickness. Transferosomes with 1:2:0.25 ratio of Phospholipid: ginger extract: Tween 80 showed particles of 318.8nm, the zeta potential of -6.3mV and 74.4% entrapment efficiency and 67% in-vitro release. Optimized batch of patches showed folding endurance 7, 0.134 ±0.0012 mm thickness, 97.18 ± 0.78% drug content. Thus, transferosomes with transdermal delivery is a better approach to deliver ginger...
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Posted by admin on May 31, 2021 in |
Aframomum melegueta (Alligator pepper) is a tropical plant that belongs to the Zingiberaceae (Ginger) family, consumed for its culinary and health benefits. This study investigated the therapeutic efficacy of ethanolic seed extract of Aframomum melegueta at ameliorating paraquat-induced ovarian toxicity in rat models. Thirty female Wistar rats were divided into control and five test groups (n=5). Group 1 (Control) received feed and water, while other groups (2-6) were treated orally and daily for 4 weeks as follows; group 2 (400 mg/kg b.w. of Aframomum meleguate seed extract); group 3 (200 mg/kg b.w. of Aframomum meleguate seed extract); group 4 (20 mg/kg b.w. of paraquat); group 5 (co-administration of 20 mg/kg b.w. of paraquat and 400 mg/kg b.w. of Aframomum meleguate seed extract); group 6 (co-administration of 20 mg/kg b.w. of paraquat and 200 mg/kg b.w. of Aframomum meleguate seed extract). At the end of the experiment, oxidative stress markers (malondi-aldehyde (MDA) and superoxide dismutase (SOD)), estradiol levels, and histology of the ovaries were assessed. Data obtained were analyzed using...
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Posted by admin on May 31, 2021 in |
A simple reverse phase SI-HPLC method was developed for the simultaneous estimation of antidiabetic combination of metformin and gliclazide. The method was based on HPLC separation on a reversed-phase C18 column using a mobile phase consisting of phosphate buffer: acetonitrile (40:60, v/v) at a flow rate of 0.8 ml/min. UV determination was achieved at 240 nm. The analytes were subjected to various forced degradation following ICH guidelines. Degradation of metformin was observed under acidic, basic, and peroxide stress. Separation of all the degraded products was achieved. Method was linear in a concentration range of 100-700 µg/ml for metformin and 20-140 µg/ml for gliclazide. The limit of detection and quantitation for metformin was 43.59 µg/ml and 132.09 µg/ml and for gliclazide 8.96 µg/ml and 27.15µg/ml. According to ICH guidelines, the developed method was validated. Metformin and gliclazide show no interference with the degradation product formed from stress studies. In view of the extended applications of the proposed method for in-vivo drug-drug interaction studies, the method was also studied for elution...
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Posted by admin on May 31, 2021 in |
The aim of the study was to evaluate the anti-diabetic and antioxidant effects of aqueous extract of Phaseolus vulgaris L. seeds in normal and streptozotocin (STZ) induced diabetic rats. Female Albino Wistar rats were divided into 6 groups of 6 rats; each was assigned into diabetic and non-diabetic groups. Diabetes was induced in rats by single intraperitoneal administration of STZ (55 mg/kg body weight). Phaseolus vulgaris L. extract at the doses of 100, 200, and 300 mg/kg body weight and glibenclamide (600 μg/kg b.w.) by intragastric tubation orally administered to both diabetic animals for a period of 30 days. After completion of experimental length, blood serum, liver, and exocrine gland were used for evaluating organic chemistry. Oral administration of Phaseolus vulgaris L. seeds significantly reduced elevated serum glucose, renal, liver function levels and significantly increased serum insulin and body weight as well as improved lipid profile due to...
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Posted by admin on May 31, 2021 in |
Objective: To study the influence of paracetamol on analgesia when given separately with low dose etoricoxib for treatment of acute pain in mice. Materials and methods: Animals were divided into 6 groups consisting of six animals in each group. Dose was calculated by using conversion factor for mice from human dose. Group I, II, III and IV received 2% Gum acacia 10ml/kg, paracetamol 84.5mg/kg, etoricoxib 3.9 mg/kg and 7.8 mg/kg respectively. Group V and VI received etoricoxib 3.9 mg/kg + paracetamol 42.25mg/kg and etoricoxib 3.9 mg/kg + Paracetamol 84.50 mg/kg respectively. Group II, V and VI were administered rescue paracetamol at 6 h. Acetic acid induced writhing test and hot plate method were used to evaluate immediate and delayed analgesic activity respectively. Results: After 1 h low dose paracetamol in combination with low dose etoricoxib showed significant (P<0.05) analgesic activity as compared to etoricoxib low and paracetamol high dose when used alone and comparable analgesia as compared to etoricoxib high dose when used alone. High dose paracetamol with low...
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