Posted by admin on Feb 28, 2021 in |
RP-HPLC method has been developed for separation and quantification of Metoprolol succinate, Telmisartan, and Clinidipine using Phenomenex Luna C18 column (150 mm × 4.6 mm, 5 µm) as a stationary phase with security guard cartridge C18 (4 × 3 mm) and acetonitrile: methanol: phosphate buffer pH 7.5 (45:30:25) as a mobile phase by isocratic elution at the flow rate of 1.0 ml/min. The detection was carried out at 229 nm. The drugs are eluted at retention times 2.0, 2.8, and 6.8min for telmisartan, metoprolol succinate, and clinidipine, respectively. The method is linear in the range of 10-80 μg / ml, 6.25-50 μg / ml, and 2.5-20 μg / ml with regression coefficients 0.997, 0.995, and 0.999 respectively for telmisartan, metoprolol succinate, and clinidipine. The method is precise within the acceptable limits (% RSD- 1.22). The proposed method showed linearity, accuracy, precision, specificity, robustness, LOD, LOQ, and system suitability results within the acceptance criteria of ICH. The method can be applied for the routine analysis of pharmaceutical...
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Posted by admin on Feb 28, 2021 in |
Tinospora cordifolia is one of the best medicinal plants holds most prominent place in Ayurveda Rasayana herbs and it also known as “rejuvenate herbs” because of its medicinal properties. It has been found to exhibit anti-microbial properties, its anti-microbial feature found in root, stem, and leaf extracts on pathogenic micro-organisms. Green synthesis of AgNPs can potentially eliminate chemical agents’ problems that may have adverse effects, thus making nanoparticles more compatible with the eco-friendly approach. The present study focuses on the green synthesize of silver nanoparticle, which was conducted by Tinospora cordifolia leaf extract. Characterization of silver nanoparticle was performed by Scanning Electron Microscopy. Silver nanoparticles exhibit strong anti-microbial effect against gram-positive and gram-negative bacteria such as Escherichia coli, Pseudomonas syringae, Staphylococcus aureus and Enterococcus faecalis. Both methanolic and ethanolic extract showed good antimicrobial activity against all the bacteria whereas methanolic extract had wide range of anti-microbial activity than ethanolic...
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Posted by admin on Feb 28, 2021 in |
Department of Botany, Avinashilingam Institute for Home Science and Higher Education for Women, Coimbatore, Tamil Nadu, conducted a pot culture experiment for analyzing the effect of bio-composted groundnut shell and vegetable waste on the growth impact of Pigeon pea (Cajanus cajan (L.) Mill sp. Organic wastes and by-products are renewable forms of resources generated all over the world. The present work explores the potential application of eco-friendly compost from groundnut shell and vegetable wastes. The study mainly deals with the physicochemical properties of raw and composted vegetables and groundnut shell Waste, pH and Electric conductivity, Lignin, Total Nitrogen (N), Total Phosphorous (P), Total Potassium (K), and C: N ratio. Composting was carried out with the help of Trichoderma asperelloides, Pleurotus florida, and Eudrilus eugeniae. The experiments consist of four treatments viz., C control, T1- compost 1 (Vermi-composted groundnut shell and vegetable waste (25g)) T2- compost 2 (Vermi-composted groundnut shell and vegetable waste (50g), T3-compost 3 (Vermi-composted groundnut shell and vegetable waste (75g). Chlorophyll, protein, and carbohydrate content were analyzed...
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Posted by admin on Feb 28, 2021 in |
The fruits of the plant Ficus racemosa and Ficus auriculata are consumed as a wild edible fruit and have also been used extensively in traditional medicine to treat various illnesses ranging from diarrhea, dysentery, jaundice to diabetes, piles, asthma, and urinary diseases. Gas Chromatography-Mass Spectro-metry (GC-MS) analysis of methanol extract of the fruits was carried out to identify the possible bioactive compounds. The major constituents identified in F. racemosa were 9, 12, 15-octadecatrienoic acid (z,z,z)- (14.323%); (z)6,(z)9-pentadecadien-1-ol (10.190%); Resorcinol (5.613%); n-hexadecanoic acid (2.965%) and Chloroacetic acid, dodec-9-ynyl ester (0.659%). The compounds like 9,12,15-Octadecatrienoic acid, (Z,Z,Z)- (58.216%); L-(+)-Ascorbic acid 2,6-dihexadecanoate (5.459%); Geranylgeraniol (0.432%); 9,12-Octadecadienoyl chloride, (Z,Z)- (0.151%) and 2H- Benzo [f]oxireno [2, 3-E] benzofuran-8 (9H)-one, 9-[[[2-(dimethyl-amino) ethyl]amino]methyl]octahydro-2,5a-dimethyl- (0.132%) were identified in F. auriculata. The Fourier-Transform Infrared Spectroscopy (FT-IR) analysis indicated the presence of N-H, O-H, C=C, C=O, C-H, C-O, S=O, C-N, and N-O functional groups. The results confirm the presence of bioactive components, which are known to exhibit medicinal value as well as pharmacological...
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Posted by admin on Feb 28, 2021 in |
Heparin is a mixture of glycosaminoglycan (GAG) chains originating from the porcine intestinal mucosa. It is used therapeutically as an anticoagulant for the treatment and prevention of thrombosis. Glyco-saminoglycans such as heparin (H) and heparan sulfate (HS) are considered attractive therapeutic agents because they modulate many biological processes and have been implicated in numerous pathologies, including cardiovascular, cancer, inflammation, metabolic, and neurodegenerative diseases and viral infections. These biological functions are believed to be dependent on the interaction of these linear polysaccharides with key proteins such as growth factors, cytokines, proteases, adhesion proteins, lipid binding proteins, etc., which have a heparin-binding domain in common and are termed heparin binding proteins (HBPs). The variability in unfractionated heparin’s pharmacokinetic properties and pharmacological effects led to the development of low MW heparin (LMWH), which is a degraded product of heparin using chemical or enzymatic cleavage techniques. The most common form of LMWH in the U.S. is enoxaparin, which is produced by β-eliminative cleavage of the benzyl esters of porcine mucosal heparin under alkaline...
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