Posted by admin on Apr 30, 2021 in |
The aim of this research was to examine the protective effect of D-Pinitol on haematological profile against Doxorubicin-induced cytotoxicityin mice. Doxorubicin is a chemotherapeutic agent valuable in treating various cancers regardless of its toxicity to normal cells, including hematopoietic suppression. Oxidative stress and generation of reactive oxygen species appear to be the reason for Doxorubicin-induced cytotoxicity in bone marrow cells. D-Pinitol is a richly available carbohydrate in Soybean plants and it has been shown to possess free radical scavenging activity and antioxidant effect in previous researches. Sixty Swiss Albino mice were divided into ten groups: Control, Doxorubicin (5 mg/kg), D-Pinitol (100 mg/kg, 200 mg/kg, 300 mg/kg and 400 mg/kg) and D-Pinitol (100 mg/kg, 200 mg/kg, 300 mg/kg and 400 mg/kg) + Doxorubicin (5 mg/kg). Blood samples were taken from the mice to determine the blood levels of haematological parameters such as white blood cells, lymphocytes, monocytes, red blood cells, hemoglobin, hematocrit and platelets. The D-Pinitol-only treated groups reported no significant variations in haematological parameters compared to the control group....
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Posted by admin on Apr 30, 2021 in |
Aim: To estimate the impact of clinical pharmacist mediated patient counseling in dyslipidemia patients in RIMS hospital, Kadapa. Objectives: To conduct a randomized study on patients. To categorize patients on demographic basis, comorbidities. To compare the lipid levels based on 20 mg and 40 mg doses of atorvastatin, the outcome based on lipid profile. Methodology: It is a prospective observational, comparative type of study. Conducted in the department of general medicine, Rajiv Gandhi institute of medical sciences at Kadapa with a period of Six months (July 2018 to December 2018), and the sample size was 72 subjects. Results: A total of 72 patients were included in the study 86.11% were male, and 13.88% were female. Average age was found to be 57.11 years. Average BMI of males is 25.4 and females is 27.17 .36 patients included in group A (statin therapy) and 36 patients included in group B (statin + patient counseling). The P value of lipid levels in group A, and group B with two different doses of...
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Posted by admin on Apr 30, 2021 in |
According to recent studies, the antioxidant related properties of Taxus baccata L. needle extracts are remarkable making it a possible raw material for the pharmaceutical industry, hence requiring the identification of major bioactive compounds and the assessment of the antioxidant properties of the extracts. The aim of this study was to investigate for the first time the individual flavonoids and the in vitro free radical scavenging activity of the methanol needle extracts from two Algerian Taxus baccata populations. The identification process was carried out using liquid chromatography coupled to electrospray ionization tandem mass spectrometry (LC–ESI–MS/MS). A total of twenty compounds have been characterized and identified. Some of which have never been previously reported in T. baccata L. The total flavonoid contents in Taxus baccata showed significant differences (p<0.05) according to the populations. The total flavonoid contents were relatively greater at the Chrea population (TFC = 220.1 ± 6.36 mg RE/g dry extract) compared to the Tikjda population (TFC = 166.6 ± 1.73 mg RE/g dry extract). Similarly, the highest...
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Posted by admin on Apr 30, 2021 in |
Deoxycholic acid (DCA) is a secondary bile salt that is a major active constituent of Chinese traditional medicine, “Niuhuang” which is known to have immunoregulatory and anti-inflammatory properties. Although data from the literature indicate possible immunomodulatory effects of Deoxycholic acid on the immune system, little has been reported regarding the mode of activity of DCA in modulating the immune system. The objective in the present study was to determine the effect of Deoxycholic acid (DCA) on immune parameters in mice and rats. The current study employed both in-vitro and in-vivo protocols in mice and rats. In-vitro study results indicate that DCA causes splenocyte proliferation at lower doses but is cytotoxic to splenocytes at higher doses were observed in these supernatants that were sensitized with DCA versus splenocytes derived from control animals. Significant differences between normal and DCA treated animals were observed in the following parameters: white blood cell indices, absolute weights of lymphoid organs, and spleen cell proliferation to DCA stimulation. These results indicate that DCA treatment modulates the...
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Posted by admin on Apr 30, 2021 in |
Levoleucovorin calcium contains amide and aldehyde groups which makes it vulnerable to degradation. Stability indicating method for determination of all seven process-related impurities and degradation impurities in Fusilev® 50 mg/mL injection was developed with the help of RP-HPLC. A Forced degradation study supports method development to ensure stability indicating conditions. Solutions of the lyophilized injection were stress-tested in different conditions like Acidic, Basic, Oxidative, Thermal, Photolytic conditions, and the mass balance was found close to 90-110%. Validation has been performed according to ICH guidelines. The method was developed in ACE C18 column (150 × 4.6 mm, 3 µm) with binary gradient elution consisting of mobile phase A, which consists a mixture of Potassium Phosphate Triabasic and Tetra butyl ammonium hydrogen sulfate in water (pH 6.8 with diluted Ortho Phosphoric Acid, 0.05 M, 1000 ml) and mixed with Methanol in ratio of 90:10 % V/V and mobile phase B consist methanol at a flow rate of 0.5 mL/min and detection performed at 280 nm with column thermostat 40 °C, injection...
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