Posted by admin on Mar 31, 2021 in |
Mebendazole is a broad-spectrum anthelmintic drug that is widely used in the treatment of helminthic infections. A simple, accurate, and precise UV-visible spectrophotometric method has been developed and validated for the determination of Mebendazole in tablet formulation. The solvent used for the development of the proposed method was Phosphate buffer 6.8 at 278.6 nm. The Beers’ law was obeyed in the concentration range 5-30 μg/ml. The developed method was validated as per ICH guidelines such as linearity, accuracy, precision, the limit of detection, the limit of quantification, and robustness. The correlation coefficient was found to be 0.999. The accuracy was found between 99.22 to 99.54%. The % RSD of Mebendazole was found to 0.05 to 0.20 for intraday and 0.05 to 0.15 for inter-day precision. The method was found to be precise as % RSD was found to be less than 2%. The LOD and LOQ were found to be 0.986 µg/ml and 2.988 µg/ml, respectively. The results demonstrated that the excipients in the commercial tablets did not interfere...
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Posted by admin on Mar 31, 2021 in |
The purpose of the present work was to prepare and evaluate olmesartan-loaded non-ionic surfactant vesicles (niosomes) to get sustained release by increasing the solubility and bioavailability. Ether injection and thin-film hydration methods were used for the preparation of all formulations as per the factorial design to study the effect of the three independent variables X1 (amounts of span 60), X2 (amount of cholesterol) and X3 (amount of chitosan/ PEG-6000) on two dependent variable Y1 (% DEE), and Y2 (% CDR), respectively. Prepared niosomes were characterized by % DEE, % CDR, FTIR, SEM, and zeta potential, etc. Statistical analysis was performed using ANOVA, and optimization was done by fitting experimental data. After 8 hours dissolution, the minimum and maximum cumulative drug release were observed to be 75.90% and 85.91%, and 77.32% and 88.74% for SPEIM-2 and SPEIM-1 and CHEIM-2 and CHEIM-1; 80.24% and 86.11% and 80.11% and 90.72% for SPTFH-2 and SPTFH-1, and CHTFH-7 and CHTFH-1, respectively. Data obtained from in-vitro dissolution tests were fitted to different kinetic models. FTIR...
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Posted by admin on Mar 31, 2021 in |
The present study was to estimate the total phenolic content, flavonoids content and evaluate the in vitro antioxidant activity of alcoholic and aqueous extract of the leaves of plant Mimusops elengi Linn. Total phenolic content was determined calorimetrically using Folin ciocalteu reagent, and Total flavonoid content was determined by aluminum chloride method. The total phenolic content of methanol, ethyl acetate soluble fraction, and aqueous extract was found 23.22, 22.44, and 15.88%w/w respectively. In the case of total flavonoid content was found 33, 31, and 24%w/w respectively. Antioxidant activity was measured based on the DPPH radical scavenging assay, Nitric oxide scavenging assay, and reducing power assay. A methanol extract of the leaves of the plant showed potent free radical scavenging activity with an IC50 value of 65.00 μg/ml. However, standard ascorbic acid activity was significantly higher than that of all extracts. The IC50 value of the standard (Ascorbic acid) was 7.779μg/ml. In the case of nitric oxide scavenging assay, the methanolic & aqueous extract of the leaves of the plant...
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Posted by admin on Mar 31, 2021 in |
By the HPTLC method, a mobile phase system comprising of Ethyl acetate: Toluene: Methanol (5:4:1v/v/v) was selected, and the detection was carried out at 280 nm. The Rf values were found to be 0.65 ± 0.03 and 0.27 ± 0.03 for Mebendazole and Atorvastatin, respectively, which showed a good separation. The method was validated as per ICH guidelines. The percentage RSD values of repeatability, intraday, and interday were found to be less than 2 prove the precision of the method. The correlation coefficient value from the calibration graph was found to be 0.9949 for Mebendazole and 0.9979 for Atorvastatin. The percentage protein binding of Mebendazole and Atorvastatin was estimated by the equilibrium dialysis method. The in-vitro displacement interaction study was carried out using standard solutions of a mixture containing 1.5×10-4 M Mebendazole and Atorvastatin along with 1.5×10-4 M BSA. The percentage protein binding of individual Mebendazole and Atorvastatin after 6hr was found to be 86.14% and 91.18%, respectively. While they were evaluated in the presence of each other, the...
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Posted by admin on Mar 31, 2021 in |
The present investigation was based on the development and evaluation of chronotropic capsules for hypertension treatment. Captopril has ACE inhibitor action to prevent the circadian rhythm related to hypertension. The solubility of captopril in the GI tract was pH-dependent i.e., captopril soluble only at 1.2 pH, show better stability, and absorbed in the upper part of the gastrointestinal tract. Captopril has 1-2 h half-life (t1/2) and 3.7 pKa value. For the optimization of formulation 32 factorial designs was used and in these methods, two independent variables at three levels were selected. The direct compression method was used for the preparation of pulsincap plugs. The dissolution profile of formulated batch F1 to F9 at the end of 9 h was found in the range of 92.50 to 99.60% in different pH of phosphate buffer. From the results, it was found that formulation F5 was shown the most similar dissolution profile because the similarity value was found to be above 90%. The swelling index was found higher in formulation F5 and...
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