Posted by admin on Mar 31, 2021 in |
Carissa carandas Linn. belonging to the family Apocynaceae, is an underutilized fruit plant. C. carandas has been used in many ethno medicines 6, 40, 30. Genetic diversity is a ubiquitous feature of all species in nature. ISSR markers are useful in areas of genetic diversity, phylogenetic studies, gene tagging, genome mapping, and evolutionary biology in a wide range of plant species 4, 12. In this investigation, 9 ISSR primers were used for fingerprinting of 9 different accessions of C. carandas grown in a different location. Of these 9 primers, the amplification of only 8 primers (4, 5, 6 and 7, HB10, HB12, HB13 and HB15) were satisfactory and reproducible. Results of the ISSR fingerprint showed 473 amplified fragments; 134 of them were polymorphic (28.33%). The sizes of the amplified fragments were ranged between 200 and 1700 bp in length. The primer HB-10 showed the highest polymorphism (46.67%), while primer 6 showed the lowest with only (8.70%). The results obtained in this study validate that ISSR is useful markers in...
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Posted by admin on Mar 31, 2021 in |
Urolithiasis is the most prevalent urinary tract disorder in males. Alfuzosin is a selective antagonist of post-synaptic α1a adrenoceptor, causing relaxation of smooth muscle in the bladder and prostate, which results in an improvement of urine flow and prevents ureter stones. The present study was aimed to evaluate the effectiveness of Alfuzosin on rats as a preventive and curative agent against the development of urolithiasis. Urolithiasis was induced by oral administration of 0.75% v/v ethylene glycol and 2% w/v ammonium chloride in drinking water for 15 days in rats. Ethylene glycol feeding resulted in hyperoxaluria and a significant (p<0.001) increased in urinary calcium, phosphate, and serum creatinine, uric acid levels. By oral administration of Alfuzosin at the dose of 1 mg/kg and 2 mg/kg for 15 days as a preventive regimen and from 8th day to 15th day as a curative regimen, significantly (P<0.01) reduced the calcium, phosphate, creatinine and uric acid levels in rats. The histopathological studies showed the disrupted renal parenchyma, degenerative changes in glomeruli, calcification in...
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Posted by admin on Mar 31, 2021 in |
A sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) technique was developed and validated for the quantification of lenvatinib in plasma samples. Plasma samples were presented by selecting the Liquid-Liquid extraction (LLE) method with ethyl acetate. The technique was developed and validated with respect to the parameters specificity, sensitivity, carry-over, recovery, precision, matrix effect, accuracy, and stability. The lenvatinib and IS (lenvatinib-D4) were eluted within 3.5 min on Symmetry-C18 column (50 mm × 4.6mm × 5µm) with 0.1% V/V formic acid in acetonitrile, methanol, and 0.1% V/V formic acid in water in the ratio of 70:20:10 as mobile phase with 0.70 ml/min infusion flow rate. This analytical method was also applied successfully to study bio-availability studies in 6 healthy rabbits. LVB was shown Tmax of 3.452 ± 0.754, mean Cmax of 89 ± 3.045, AUC0t of 1700.01 ± 257.892, and AUC0t of 3665.3 ± 259.59 for Test formulation. The method can be useful in the quantification of lenvatinib in clinical research organizations for bioavailability studies and in forensic...
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Posted by admin on Mar 31, 2021 in |
Ruellia tuberosa, commonly called Minnie root, has plenty of applications in herbal medicine due to the presence of several bioactive compounds. A simple, efficient, and reproducible in-vitro plant regeneration protocol was developed for Ruellia tuberosa. The cotyledonary nodal explants were excised from seedlings were inoculated on Murashige and Skoog (MS) medium supplemented with various concentrations of BAP and NAA, respectively. The highest numbers of 9.86 ± 0.15 shoots/explant were obtained in MS medium augmented with BAP (2.0 mg L-1) and NAA (1.0 mg L-1). The micro shoots induced per explant significantly increased with the supplementation of BAP to auxin-containing medium. The results showed that maximum percentage of multiple micro shoots 89%, with a number (13.80 ± 0.26) shoots / explant was found on MS medium supplemented with BAP (2.0 mg L-1) and NAA (1.5 mg L-1). Further, the highest frequency of micro shoots was produced on MS medium supplemented with TDZ (0.3 mg L-1) and AgNO3 (0.4 mg L-1) for large-scale production. The highest frequency of root induction (96%)...
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Posted by admin on Mar 31, 2021 in |
Coronaviruses are a group of viruses that belongs to the Coronaviridae family. These viruses have helical symmetry, enveloped, positive-sense, single-stranded RNA genome with nucleocapsid that causes diseases in birds and mammals. SARS-CoV 2 complete genome has genome size of 29,903 bp, having nine genes identified as Orf1a Polyprotein, Orf1ab Polyprotein, ORF3a Protein, ORF7a Protein, ORF8 Protein, Envelope proteins, Spike Surface glycoprotein, Membrane glycoprotein, and Nucleocapsid Phosphoprotein. Based on the ProtParam result, ORF1a has more molecular weight (489989 daltons), and Envelope protein has a less molecular weight (8365 daltons). The pI is lower in Orf1a polyprotein, Orf1ab Polyprotein, ORF3a Protein, ORF8 Protein, and Spike Surface glycoprotein) and high in ORF7a Protein, Envelope proteins, Membrane glycoprotein, and Nucleocapsid Phosphoprotein. The Orf1a Poly-protein, Orf1ab Polyprotein, ORF3a Protein, Envelope proteins, Spike Surface, and Membrane glycoprotein are stable. The higher aliphatic index and more hydrophobicity of membrane glycoprotein suggest an increase in the thermostability of protein might favor an increase in its solubility. The vaccines like EIAV vaccine gp45, anti-SARS m396 Antibody, EIAV vaccine...
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