Posted by admin on Jan 31, 2021 in |
Ethnopharmacological Relevance: Traditional herb Solanum xanthocarpum contains solasodine as the chief constituent of fruits. Since analgesic, anti-inflammatory, anticonvulsant effects of fruits are reported; study was designed to rationalize the use of solasodine as neuro-protective with its mechanism of action. Aim of Study: Cerebrovascular Diseases (CVD) includes devastating disorders like ischemic stroke, hemorrhagic stroke, and cerebrovascular anomalies. Worldwide, around 15 million people suffer a stroke every year. With about 5 million deaths, stroke is the second most common cause of death and a major cause of long-term disability. This study was carried out to evaluate the neuroprotective activity of solasodine from Solanum xanthocarpum against global cerebral ischemia/reperfusion induced brain injury in rats. Materials and Methods: Neuroprotective activity was carried out by global cerebral ischemia on Wistar albino rats, distributed into 6 groups (n=8). Rats were subjected to bilateral common carotid artery (BCCA) occlusion for 30 min followed by 60 minutes reperfusion. The antioxidant enzymatic and non-enzymatic levels, cerebral infarction area, and histopathology were evaluated. Results: Solasodine showed dose-dependent neuroprotective activity...
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Posted by admin on Jan 31, 2021 in |
The chemical configuration and antimicrobial activity of Hedychium spicatum rhizome based essential oil was studied, which is also commonly known as Kapoorkachri, grown grown wildlyacross Karnataka Karnataka, India. Thirty-eight compounds were detected from the rhizome based essential oil by GCMS. The main compound was characterized as Ethyl p-methoxy-cinnamate (50.1%), Ethyl cinnamate (26.22%), and Eucalyptol (5. 68%). The anti-bacterial activity was performed by the Broth dilution method. Oil-DMSO solution in seven dilutions was developed starting from 100%, 50%, 25%, 12.5%, 6.25%, 3.12% and 1.56% against five selected human pathogenic organisms which comprised of Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, and Candida albicans. Anti-microbial activity against these pathogens were found to be 25%,25%,25%, 50% and 1.56% respectively. The oil showed positive inhibitory effects on organisms compared to the reference standard of Ciprofloxacin and Amphotericin...
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Posted by admin on Jan 31, 2021 in |
Development and validation of a simple UV spectroscopic method for the determination of Duloxetine by the derivative spectro-photometric method is the objective of the present work. The analysis was carried out by using Shimadzu UV-1700, and Shimadzu UV-1800 UV spectrophotometer, and Acetonitrile and water (8:2) was used as solvent or mobile phase. Detection or qualitative analysis was carried out at a wavelength of 240 nm. ICH guidelines were followed to verify the method validation process by using various parameters like linearity, accuracy, precision (interday and intraday), ruggedness, robustness, the limit of detection (LOD), and limit of quantification (LOQ). According to Beer’s Lambert’s law, the derivative UV spectrophotometric method was found linear in the range of 10-50 μg/ml. The analytical method was rugged and robust with a % relative standard deviation of less than 1.8. The percentage recoveries were higher than 98.7% in all the validation parameters and conditions. Based upon the analytical experiment, the developed method was found accurate, precise and rapid, and suitable for the rapid determination of...
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Posted by admin on Jan 31, 2021 in |
Co-crystallization is one of the most reliable alternative approaches to increase the solubility of poorly water-soluble drugs without affecting their physicochemical properties. Pharmaceutical cocrystals are neutral organic compounds connected to the API preferentially by loosely formed bonds as dipole-dipole interaction. Our present study aims at improving the solubility of an effective oral hypoglycemic, Glimepiride, a sulphonylurea class of drug that often lacks water solubility. In the process of enhancing its solubility, four different co-formers were used as Anthranilic acid, Succinic acid, Salicylic acid, Benzoic acid, and Gallic acid in different stoichiometric ratios like 1:2 and 1:3. The technique opted for the making is slow evaporation and prepare the nanoparticle by using Chitosan and gelatin polymeric matrix with aldehydic oxidized Xanthan gum as crosslinking agent. Initial confirmation was made through melting point determination. Later structure elucidation of co-crystals was carried out by several analytical methods, such as FTIR, X-ray Diffraction. In FTIR spectra, a sharp decrease in the intensity of N-H peak of salicylic acid and succinic acid was observed...
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Posted by admin on Jan 31, 2021 in |
Medicinal plants are often used by humankind since their evolution. They are known to have prominent therapeutic potential. The present work is conducted on Glycosmis pentaphylla (Rutaceae) an ethnobotanically important medicinal plant rich in secondary metabolites. The plant possesses metabolites like alkaloids, flavonoids, terpenoids, etc., which can be used against various illnesses. The plant is traditionally used by the folklore people in many parts of the world. The aim of the study is to investigate the bioactive compounds present and to evaluate the significance of the therapeutic and pharmacological uses of the phytoconstituents. All the standard phytochemical and spectroscopic procedures were followed for the detection and estimation of phytoconstituents. The leaves, stem, and root of Glycosmis pentaphylla were collected and was extracted using various solvents based on their polarity. Preliminary phytochemical analysis reveals the occurrence of several secondary metabolites. The quantitative estimation exhibited the presence of secondary metabolites abundantly in leaves on comparison with stem and root. Further, an HPTLC analysis to determine the number of phytoconstituents was also...
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