Posted by admin on Nov 30, 2020 in |
Purpose: To formulate and evaluate Olanzapine immediate-release tablets using direct compression. Immediate release of drug from the dosage form is required for rapid onset of action and produce an immediate therapeutic effect. Method: Tablets were formulated using two different natural super disintegrants like Fenugreek and Isabghul and in combination by using Response Surface Methodology. The selected independent variables (Y1) Fenugreek and (Y2) Isabghul showed a significant effect on dependent variables, i.e., disintegration time (X1) and percentage drug release (X2). Drug and excipients compatibility studies were done by FTIR studies. Results: No significant drug-polymer interactions were observed in FTIR studies. The prepared tablets were evaluated for weight variation, friability, hardness, disintegration, and dissolution. A formulation containing 2% fenugreek and 5% isabghul was offered the relatively rapid release of Olanzapine when compared with other concentrations employed in this investigation. The formulation with 4.2% Fenugreek and 5% Isabghul was optimized, and the tablets were evaluated. Conclusion: The optimized formulation AOF14 showed the desired level of % CDR 98.43 at the end of...
Read More
Posted by admin on Nov 30, 2020 in |
Background: Depression is affecting around 5% of the population. In the traditional systems of medicine, many plants and formulations have been used to treat depression for thousands of years. Objectives: This study evaluated the synergic effect of Hydro-alcoholic extract of Rhizomes of Nordostachys jatamansi DC Per Se with combination with Imipramine in Wistar, albino rats, and swiss albino mice normal groups. Materials and Method: Animals of either sex were selected and randomly divided into a test group. Jatamansi extract 10:1 and imipramine dissolved in distilled water were used. Animals were tested for forced swimming test, tail suspension test, and locomotor after given test drug. Results: Nardostachys jatamansi DC, when given to rats, showed a dose-dependent increase in the number of rotations during forced swimming tests in rats. Nardostachys jatamansi DC, when given to mice dose-dependent statistically significant decrease in immobility time, swimming time, and climbing observed. When given along with a combination of imipraine it shows a statistically significant difference in the result, confirmed that it could have synergistic...
Read More
Posted by admin on Nov 30, 2020 in |
Introduction: Inhaled Corticosteroids remain the mainstay therapy for asthma. Uncontrolled asthma (UA) dose not respond to the highest inhaled corticosteroids, which remains a challenge for clinicians. Multidrug resistance-associated protein 1 (MRP-1) are molecular “pumps,” which expel toxins and modulate corticosteroids’ pharmacokinetics and may lead to UA. Moreover, their role in UA is still not clear and needs further investigation. Our study evaluated the expression and functionality of MRP-1 in patients with controlled asthma (CA) and UA. Materials and Methods: UA was defined as a lack of control with high dose inhaled corticosteroids along with the use of other controller medications. Whole blood MRP-1 expression and functional activity on peripheral blood mononuclear cells (PBMCs) in UA (n=38), (mean age = 53.53 ± 11.85, male = 18) patients, CA) (n=60) (mean age = 51.65 ± 16.99, male = 31). MRP-1 was analyzed by flow cytometry. Result: Expression of MRP-1 positivity on lymphocyte gated population was significantly higher in UA than CA (p<0.001). Similarly, the functional activity of MRP-1 was significantly increased...
Read More
Posted by admin on Nov 30, 2020 in |
Polyherbal formulation (HF) containing different herbs have been used to treat diabetic patients by ayurvedic practitioners in India. The present study reports the effect of HF alone and with metformin on different rat models of hyperglycemia. HF treatment with HF alone and in combination with metformin elicited a significant hypoglycemic effect on epinephrine hyperglycemia and alloxan diabetic rats. HF treatment also altered the glucose-tolerance curve pattern both in normal and diabetic rats. Further, HF treatment alone and with metformin increased liver glycogen, glucose transfer in the liver, enhanced glucose uptake process in peripheral muscles, and also improved architecture of β-islets of Langerhans of diabetic rats. The combination of HF with metformin elicited a greater degree of hypoglycemic activity, which was reflected both on blood glucose level as well as other biochemical parameters studied. The hypoglycemic effect of HF may be mediated through pancreatic as well as extrapancreatic systems. Further, HF combination with metformin seems to be useful in diabetes mellitus and needs to be investigated on human subjects to...
Read More
Posted by admin on Nov 30, 2020 in |
An attempt has been made in the present study to isolate and identify actinomycetes, which can produce bioactive metabolites possessing antimicrobial activity. The soil samples were collected from mangrove sediments of Krishna district, Andhra Pradesh. The potent secondary metabolite producing strains were isolated and designated as VLK-15, VLK-24, and VLK-56. Identification of the strains was carried out basing on morphological, cultural, physiological and biochemical characters. Phylogenetic analysis of 16S rRNA gene sequence showed that the strains VLK-15, VLK-24, VLK-56 form distinct clade within the genus Streptomyces. The 16S rRNA sequences were deposited in the GenBank database of NCBI under the accession numbers MG309759 (Streptomyces olivoverticillatus VLK-15), MG309760 (Strepto-myces rubrus VLK-24), and MF952650 (Streptomyces sampsonii-VLK-56). The antimicrobial activity of the strains was evaluated by using agar well diffusion assay. The ethyl acetate extracts were highly effective against Candida albicans, Staphylococcus aureus, Bacillus megaterium followed by Bacillus subtilis, Escherichia coli and Klebsiella...
Read More
