Posted by admin on Sep 30, 2024 in |
The aim of this study was the quantitative phytochemical screening of extracts obtained from the leaf, flower, and latex with antibacterial activity and antioxidant activity of Cascabela thevetia (C. thevetia). The Quantitative determinations of total flavonoid, carbohydrate, and polyphenol contents were accomplished by Folin-Ciocalteau, phenol-sulfuric acid, and AlCl3 methods. The in-vitro antioxidant activity was evaluated by the DPPH free radical scavenging activity method. All the samples were screened for antimicrobial activity against gram-positive (S. aureus, S. pneumonia) and gram-negative (E. coli and K. pneumoniae) bacterial strains by using the disc diffusion and well method followed by calculation of minimum bactericidal concentration (MBC). Phytoconstituents of different samples of C. thevetia in different solvents contain total polyphenol and carbohydrate content were highest at methanol flower extract 80.26 ± 0.86 and 178. 15 ± 3.15 µg GAC and GLU equivalent/mg of dry extracts weight respectively. But flavonoid content was methanol leaf extract 654.53 ± 2.08µg QAR equivalent/mg of dry extracts weight. Among them the lowest IC50 value was 82.84 performed by aqueous...
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Posted by admin on Sep 30, 2024 in |
Introduction: Pathogenic infections place a significant burden on the healthcare system since they are linked to high rates of morbidity and mortality. Successful therapy of these illnesses depends on an early diagnosis and a good antimicrobial resistance testing. Therefore, the goal of the current investigation was to assess aerobic bacteriological profile and antibiogram from a variety of sterile fluids. Material and Method: This observational study was carried out in the Department of Microbiology, Adesh Institute of Medical Science & Research Bathinda, Punjab from 20 September 2023 to 15 February 2024 obtaining the approval from Ethics Committee of Biomedical & Health Research, Adesh University. All the Body fluid samples were received in Bacteriology Laboratory from various departments. All body fluid samples are processed by Bact alert system. Positive samples were further subculture on blood agar and MacConkey agar. Gram Staining was done to identify gram-positive bacteria and gram-negative Bactria, further antimicrobial susceptibility was done by Vitek 2 compact system as per CLSI guidelines 2023. Results: A total of 288 body...
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Posted by admin on Sep 30, 2024 in |
Introduction: Flap surgeries can be followed by some complications due to the infection of the operating site. Sutures at the operating site can predispose to surgical site infections by harbouring the microorganisms due to their wicking action. Triclosan-coated antibacterial sutures can be used an effective substitute to traditional sutures due to its ability to reduce biofilm. Aim: This trial evaluates clinically and microbiologically the effectiveness of antibacterial coated sutures compared to non-coated ones. Materials and Methods: 20 sites in 10 systemically well patients were diagnosed with Stage III Periodontitis [2017 AAP Classification] and was allotted into group A and group B randomly in a split-mouth design for the research. Flap surgery was performed, and in Group A, triclosan-coated polyglycolic acid braided sutures were placed and in Group B non-coated polyglycolic acid braided sutures were placed. In both the groups, Visible Plaque Index (VPI) and total microbial colony count on the suture were assessed on the suture removal day. Healing Index (HI) was assessed on the 8th day and day...
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Posted by admin on Aug 31, 2024 in |
Ovarian cancer, a major concern in India, is among the leading causes of cancer-related deaths in women. Early detection is crucial due to frequent late-stage diagnoses. Red Cell Distribution Width (RDW), a marker for red blood cell size variability, is promising but lacks extensive study in ovarian cancer. This research aims to correlate RDW with hematological parameters in ovarian cancer patients, potentially improving early detection and prognosis. This study examined data from ovarian cancer patients at Mc Gann’s Teaching District Hospital in Shivamogga. Hematological parameters, RDW, were obtained from records, with imaging and histopathological analysis confirming diagnoses. Inclusion criteria Involved ovarian malignancies or benign tumors, while exclusions involved comorbidities or recent treatments impacting blood parameters. Significant differences were observed in RDW (p = 0.033), Platelet-to-Lymphocyte Ratio (PLR) (p = 0.004), Neutrophil-to-Lymphocyte Ratio (NLR) (p = 0.001), Lymphocyte count (L) (p = 0.0006), and Neutrophil count (N) (p = 0.003) among the case, control, and normal groups. Post hoc analysis revealed significant disparities between the case and control groups for...
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Posted by admin on Aug 31, 2024 in |
Silymarin, an intricate blend of flavonolignans obtained from Silybum marianum, or milk thistle, has drawn a lot of interest because of its possible medical uses. This study reports on the development and validation of an analytical technique that employs high performance thin layer chromatography (HPTLC) to estimate silymarin in pharmaceutical dosage forms (tablet) and bulk drug. Chloroform: methanol (6:4 v/v) was the solvent system used in the chromatographic separation of the medication on aluminium plates pre-coated with silica gel 60 GF254, (10 cm × 10 cm with 250 μm layer thickness) as the stationary phase. At 288 nm, a densitometric analysis of the divided zones was carried out. Densitometric scanning was carried out at 288 nm using a Camag TLC scanner 3, which was controlled by Win CATS software. The radiation source utilised for the scan was a deuterium lamp with slit size of 5.00 x 0.45 mm. It was discovered that the retention factor was 0.42. In the 200–1200 ng/band range, linearity R2= 0.993 was used to evaluate...
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