Posted by admin on Dec 31, 2020 in |
The main aim of the present research work is to develop a sensitive, precise and accurate HPLC (High-Performance Liquid Chromatography) procedure for the selective estimation of sonidegib in both human plasma and mobile phase. An isocratic separation of sonidegib through a 5µ Zorbax C-18 analytical column with the dimensions of 25 cm × 4.6 mm utilizing mobile phase composition of methanol, water and acetonitrile at a ratio of 10:10:80 v/v. The detection of the analyte was processed at the maximum wavelength of 254 nm and with 1 ml/min flow of the mobile phase. The drug was eluted from the column at the retention time of 3.6 min in plasma samples and 4.81 min in movable phase. Five variable concentration levels of 5, 10, 15, 20, and 25 μg/ml were used for the estimation of recovery and linearity. The recovery findings were 5.03, 9.93, 14.96, 20.14, and 24.91 μg/ml, respectively, for Sonidegib in the mobile phase. Similarly, 6 concentration levels of 20, 40, 200, 400, 800, and 1200 μg/ml were...
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Posted by admin on Dec 31, 2020 in |
Plant diseases caused by microbial pathogen leads to severe economic loss. Attempt to synthesize nanoparticles for this purpose is an economical and effective means to control these pathogens. For this purpose, nanoparticles synthesized via biological means are not only a safer choice but also a big step towards conservation of the integrity of environmental elements. In the present investigation, a successful attempt has been made to synthesize and characterize copper nanoparticles biologically synthesized from Lawsonia inermis leaf extract by an eco-friendly method. Formation of copper nanoparticles is confirmed by UV–Vis absorption spectroscopy, shape, and size studies were carried out Scanning electron microscopy (SEM), Transmission electron microscopy (TEM) analysis. TEM analysis revealed that copper nanoparticles were 2-5 nm in size and X-ray Diffraction studies confirmed the crystal structure of the nanoparticles. Functional groups and the chemical composition of associated molecules were studied by FTIR analysis. Further research involves the implementation of this research to control some plant diseases caused by pathogens that have developed resistance against present available bio-control...
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Posted by admin on Nov 30, 2020 in |
Sickle cell anemia is a major life-threatening hemoglobin disorder in the world. This hemoglobin disorder is an autosomal recessive type, therefore considered as a major health problem in the world. The pain symptoms and complications of sickle cell anemia can be life-threatening many times. Management of sickle cell anemia demands drugs to be safe, well-tolerated, lesser side effects, and effective in improving the quality of life in patients. The alternative system of medicine can be the fruitful therapeutic option in conditions where the drug has to be consumed for life long. The T-AYU-HM Premium is a novel traditional Ayurvedic medicine designed on such a purpose to reduce clinical complications and enhance the quality of life in sickle cell anemia patients. The study conducted was a single dose oral toxicity study of T-AYU-HM Premium to assess its safety profile at a high dose. The study was conducted in compliance with the OECD principles of GLP (1998). The study was conducted according to OECD for testing of chemicals, N-425 acute oral...
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Posted by admin on Nov 30, 2020 in |
The inhibition of the protein cyclooxygenase (COX) is a well-known mechanism for achieving analgesia. Selective inhibitors of cyclooxygenase-2 (COX-2) provide excellent analgesia but can have side-effects. In this study, we chose ibuprofen, a non-selective COX inhibitor that has been safely used for a long time, to develop a novel, selective COX-2 inhibitor. The 3-dimensional structure of the drug target, COX-2, was obtained from the RCSB PDB online database and was input in SeeSAR. Three hundred and thirty alterations were made to ibuprofen molecule. Initial docking was performed in SeeSAR and CLC Drug Discovery Workbench to get a docking score. The 216 ligands that bound to COX-2 with the best binding score were then docked to cyclooxygenase-1 protein (COX-1), and a score was generated based on the binding affinity. Twenty-six of these molecules that didn’t bind to COX-1 were chosen as the selective inhibitors of COX-2 and these were tested for drug-likeness properties using the DruLiTo software. Twenty-two compounds with good drug-likeness properties were subjected to ADMET verification. 5 compounds...
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Posted by admin on Nov 30, 2020 in |
Streptozotocin is 2-Deoxy-2-(3-methyl-3-nitrosoureido)-D-glucopyranose drug is commonly used to induce diabetes in experimental rats. This study was designed to evaluate the role of β-endorphin and C-peptide in Acu-TENS therapy at Zusanli (ST36) acupoint. Diabetic was induced by Streptozotocin (45mg/kg/body weight) with a single dose of intraperitoneal injection in male albino Wistar rats. The diabetic rats were treated with Acu-TENS electrical stimulation with a pulsed current of low frequency and high intensity at bilateral Zusanli (ST36) acupoint without anesthesia for 60 days, and the effect was compared with pioglitazone (7.5 mg/kg/body weight) treated diabetic rats on fasting blood glucose, β-endorphin & C-peptide levels. The results showed that a significant reduction (p≥0.01) in fasting blood glucose and increased β-endorphin and C-peptide level in Zusanli (ST36) acupoint treated diabetic rats. The anti-diabetic effect of Zusanli (ST36) acupoint may be due to stimulation of Qi (life energy) and regulating the circulation of blood and an increase in insulin secretion through the mediation of β-...
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