Posted by admin on Oct 31, 2020 in |
Free radicals are unstable molecules that are formed during normal cell metabolism through chemical changes that take place in a cell. This causes damage to cellular components such as cells, proteins, and DNA, or the cell membrane. Free radicals play a role in a complex interplay of different mechanisms in both normal agings, neurodegenerative, and other diseases. Aqueous and methanolic solvent extracts of pulp (without seed), pericarp, leaf, and seed of Aegle marmelos were subjected to phytochemical screening tests, quantitative determination of total phenol content (TPC), total flavonoid content (TFC), total flavonol content (TF) and in-vitro antioxidant activities such as DPPH, hydrogen Peroxide (H2O2), reducing power assay (RPA), ferric ion reducing antioxidant power (FRAP), ABTS and phosphomolybdenum total antioxidant assay (PM) by using standard procedures for the detection. The aqueous extract of pulp was found to possess maximum TPC (23.88±0.91mg GAE/g), TFC (63±0.25mg QE/g) and TF (13.30±0.53mg QE/g) values for methanolic leaf extract, respectively. The lower EC50 values were observed for DPPH assay for aqueous extract of seed is 160.117±1.62...
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Posted by admin on Oct 31, 2020 in |
The objective of this study was to formulate a Novel drug delivery device that is capable of releasing the drug in controlled kinetics through the oral route, bypassing the acidic environment of the stomach. Therefore, it was planned to develop an osmotic device with an enteric coating. It was projected to enhance the strength of the semi-permeable membrane for the osmotic device to control drug release for a longer time at controlled release kinetics. The formulations were designed and optimized by using OFAT design to find out the best formulation. The drug release rate was taken up to 8 h, and a comparative study of drug release with a marketed product was carried out. The drug release kinetics showed the optimized device to follow zero-order release kinetics. The FT-IR studies revealed that no physicochemical interaction between excipients and drugs. Stability studies showed that optimized formulation was stable. The observed drug release pattern was found significantly similar at a later stage to release the pattern of a marketed product. The...
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Posted by admin on Sep 30, 2020 in |
In recent work, an ethanolic extract of okra seeds was successfully conjugated onto gold nanoparticles (AuNPs) via a reduction reaction that has been developed by our laboratory. Because of eliciting antioxidant activity and the presence of g-sitosterol in the extract, such synthesis methodology could be applied. The resulting okra-AuNPs were physically characterized by different methods, including dynamic light scattering (DLS), Zeta potential measurement, UV-Visible spectrophotometry, Transmission Electron Microscopy (TEM), Helium Ion Microscopy (HIM), and Fourier Transform Infrared (FTIR) spectroscopy. The other two biological characterization techniques, such as thioflavin assay and cell viability test, were also performed. Results indicated that the okra-AuNPs were spherical with a mean diameter of around 35.6 nm. A maximum absorption wavelength (lmax) was at 521 nm. A zeta potential was determined to be around -59.2 ± 2.9 mV. Binding between the okra-AuNPs and islet amyloid polypeptide (IAPP) was observed, resulting in IAPP aggregation and reduction of β-sheet contents of the aggregated polypeptides. It seemed that the okra-AuNPs exhibited cytoprotective activity on pancreatic beta cells by...
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Posted by admin on Sep 30, 2020 in |
New simple, sensitive, and validated stability-indicating RP-HPLC method has been developed for the simultaneous estimation of Rilpivirine and Dolutegravir in its bulk form. Chromatographic separation was achieved on a Hypersil ODS (250mm × 4.6mm i.d., 5µm) maintained at ambient temperature by a mobile phase consisted of methanol and water (80:20v/v) and a flow rate of 1.0mL/min with a load of 20µL. The detection wavelength was set at 282nm. The retention time for the drugs was found to be Rilpivirine (5.14min), Dolutegravir (6.72min). The eluted compounds were detected using a UV detector. The drugs were subjected to stress degradation as per ICH Q1A. There was the interference of degradant at RT of Rilpivirine and Dolutegravir. The developed method was successfully validated according to ICH guidelines. The calibration curve was found to be linear over a range of 10-100µg/mL. The accuracy of the method is indicated by a good recovery in the range of Rilpivirine 99-102% and of Dolutegravir 99-102%. The limit of detection and limit of quantification of Rilpivirine was...
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Posted by admin on Sep 30, 2020 in |
The present study was undertaken to systematically analyze, document the traditional knowledge of Clerodendrum species use for the treatment of various human ailments from NER. The information was collected by literature survey as well as by consulting questionnaire with the villagers and local communities of NER. A total of twelve Clerodendrum species were collected, and among them, C. colebrookianum, C. indicum, C. viscosum were found to have multi-medicinal values that were widely used for the treatment of various diseases. Further antidiabetic properties of Clerodendrum species were evaluated by α-amylase and α-glucosidase assay. The result showed 4 species, C. indicum, C. japonicum, C. serratum, C. viscosum exhibited significant (>50%) α-amylase inhibition properties while 3 other species, C. colebrookianum, C. inerme, C. viscosum displayed significant α-glucosidase inhibition properties. Lowest IC50 values were observed in C. serratum of α-amylase (45µg/ml) and C. viscosum of α-glucosidase (47 µg/ml). A detailed scientific investigation of the biochemical compounds and metabolites with potential biological activities in Clerodendrum species may lead to the discovery of potential drug...
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