Posted by admin on Dec 31, 2020 in |
In recent years, scientific research has confirmed the presence of large amounts of probiotic Lactobacillus sp. in fermented dairy products, which shows a positive impact on human health. This study was designed to isolate Lactobacillus with probiotic potential from local and commercial samples. 40 Lactobacilli strained were isolated from commercial and local yogurt samples by tenfold dilution plating method and plated on Man-Rogosa-Sharp agar medium and were screened for their use as potential probiotics. The isolates were tested for their ability to survive at pH 2.0, pH 3.0, in the presence of 0.3% bile salt and antimicrobial activity; sensitivity against 10 specific antibiotics (ciprofloxacin, erythromycin, tetracycline, penicillin G, ampicillin, streptomycin, polymyxin B, vancomycin, chloramphenicol and rifampicin) using filter paper disc diffusion method and two-fold serial dilution methods. Out of the 40 strains, 24 strains (42%) had survival rates above 90% after 2 h of incubation at pH values of 2.0 or 3.0 pH. Further screening performed on the above 40 isolates indicated that 24 strains show to 0.3% bile salt. Lactobacilli...
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Posted by admin on Dec 31, 2020 in |
Novel Di and Tridentate chiral molecules and its analogues were designed and synthesized in that the chiral amino ester and 2-amino-3, 5-dibromobenzaldhehyde were condensed. Further, it has coupled with Cassic acid to form desired active molecules. The developed compounds are evaluated for molecular docking to check potency and may represent potential drug candidate inhibitors of RNA dependent RNA polymerase of Covid-19, compare to Remdesivir and Hydroxychloroquine. The present work is to identify and investigating the potential drug out of eight compounds that may be used to inhibit RNA-dependent RNA polymerase non-structural proteins complex of Covid-19 to prevent the replication of viral genome in host cells. The compounds BNT-202006, BNT-202008 & BNT-202009 showed and exhibited minimum binding energy when it bound with protein targets when compared with Remdesivir and Hydroxychloroquine as standard...
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Posted by admin on Dec 31, 2020 in |
The rapid increase in multi-drug resistance (MDR) infections present a challenge in the development of therapies against them. Antimicrobial peptides (AMPs) can be the answer to this challenge. AMPs play an important role in host defense mechanisms. Plants are the precious source of natural antimicrobial molecules, including antimicrobial peptides known as Plant Antimicrobial Peptides (PAMPs). The present research work was aimed to isolate antimicrobial proteins or peptides from the leaf and flower extract of Bougainvillea ‘Texas King’ and Bougainvillea ‘Shubra’ cultivars. Protein pellets obtained after 50% and 25% ammonium sulfate precipitation of B. ‘Shubra’ leaf and flower extract showed maximum total protein of 311 µg/ml and 798 µg/ml respectively and 25% and 50% protein pellet of B. ‘Texas King’ leaf and flower extract showed maximum total protein of 529 µg/ml and 904 µg/ml respectively. Different protein pellets and supernatant were screened for antimicrobial activity against selected microorganisms. The protein samples having antimicrobial activity were electrophoresed on 12% Tricine SDS-PAGE to estimate the molecular masses of the antimicrobial protein/peptides. Molecular...
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Posted by admin on Dec 31, 2020 in |
The gradient HPLC method was developed to detect and quantify four related substances (FIP, FHO-2, STG-01, and desfluoro) in ezetimibe drug material. The efficient separation was carried out on Water X-Select CSH C18 column (4.6 mm × 100 mm, 2.5 μm). The impurities were separated in gradient mode of elution with 0.1% trifluoroacetic acid in water (mobile phase A) and 0.1% trifluoroacetic acid in acetonitrile (mobile phase B) at a flow rate of 1.0 ml /min. The effluents were detected at 254 nm. The method has been validated in compliance with the regulatory standards suggested by the International Conference on Harmonization. The parameters validated included precision, linearity, detection limit, quantification limit, specificity, accuracy, and robustness. The method showed good linearity from 0.03 μg/ml to 1.5 μg/ml for FIP, FHO-2, STG-01, and desfluoro. The developed method was applied to determine the studied impurities in three different batches of ezetimibe drug material. The method proposed in this work could be employed in quality control of ezetimibe bulk...
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Posted by admin on Dec 31, 2020 in |
An analytical method consists of a detailed, stepwise list of instructions to be followed in the qualitative, quantitative, or structural analysis of a sample for one or more analytes and using a specified technique. A novel, simple, precise, sensitive, and reproducible RP-HPLC method for the simultaneous estimation of Dapagliflozin and Saxagliptin in bulk and pharmaceutical formulation was developed and validated. The separation was carried out on Symmetry C8 (4.6 × 150 mm, 3.5 µm, Make: XTerra) column with buffer: acetonitrile in the ratio of 70:30 %v/v (pH 3) as the mobile phase at the flow rate of 1 ml/min. The eluent detection was carried out using a UV-Visible detector at 221 nm. The retention time of Dapagliflozin and Saxagliptin was 2.83 min and 4.35 min, respectively. Linearity was observed Dapagliflozin and Saxagliptin in the concentration range of 25-125 μg/ml and 12.5-62.5 μg/ml, respectively. The % mean recovery of Dapagliflozin and Saxagliptin was found to be 99.90 and 99.99, respectively. The present study demonstrates the applicability of chromatographic method to...
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