Posted by admin on Sep 30, 2020 in |
The facts like developing resistance in bacterial pathogens against conventional antibiotics, emergence of new infectious agents etc. have been leading the scientists to tedious research activities to find out antimicrobial drugs with novel modes of action. Among the multidrug-resistant (MDR) bacterial species that pose threat to human life, the IDSA has identified a group pathogens known as ESKAPE pathogens which show extensive drug resistance through a wide range of mechanisms. Silver nanoparticles (AgNPs) have been identified as very effective and to avoid the chemical contamination in preparation of them, green synthesis is being used. In the present study, AgNPs using Picrorhiza kurroa Royle ex Benth were synthesized using reduction method. The green synthesized AgNPs were characterized by UV–Vis, DLS-zeta potential and FESEM analysis. Anti-ESKAPE activity of the synthesized nanoparticles was also performed. FESEM studies revealed that the nanoparticles were spherical and nanosize in shape. The UV–Vis spectral studies confirmed the surface plasmon resonance of green-synthesized silver nanoparticles. The particle size was found to be 267 nm and the stability...
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Posted by admin on Sep 30, 2020 in |
Active Pharmaceutical ingredient (API) of pharmaceutical bulk drug Brompheniramine Maleate (API) was validation by gas chromatography. Aim for this article was to check the Residual Solvents in Brompheniramine Maleate (API) by Gas Chromatographic technique. To validate a Gas Chromatographic method for detection and Quantification of Residual Solvents Methanol, Isopropyl Alcohol, DMSO and -O-Xylene. This technique was developed accurately, and validation parameters such as Accuracy, Specificity, Precision, Linearity and Range, Limit of detection (LOD), Limit of quantitation (LOQ), ruggedness, robustness and system suitability testing are explained. Gas chromatograph equipped with FID detector and headspace injection and column 30 m × 0.32 mm ID × 1.8 µm DB-624 capillary column with column temperature was 40 °C (hold 10 min) to 250 °C @ 40 °C/min, hold at 250 °C for 5 mins. Specificity was retention time for Methanol (2.13), Isopropyl Alcohol (3.45), DMSO (13.90), and -O-Xylene (13.74). The recovery for Methanol % RSD was 2.49, Isopropyl alcohol 1.18, and O-Xylene 1.91. All validation parameters are used in the routine and stability...
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Posted by admin on Sep 30, 2020 in |
The secondary plant metabolites possess therapeutic value, thus play a significant role in human health and general wellbeing. The present study aimed to estimate the secondary metabolite concentration and to evaluate the antioxidant potential of Tithonia diversifolia leaf and flower extract in methanol. The phytochemical compounds in the extracts were characterized through Gas Chromatography-Mass Spectrometry (GC-MS) analysis. The leaf extract exhibited sixteen bioactive compounds, and flower extract revealed thirteen compounds with valuable activity. The major phytocompounds in leaf extract were pentacosane (22.01%), gamma- sitosterol (15.80%) and pentatriacontane (11.95%) whereas in flower extract showed methyl linolelaidate (19.55%), methyl palmitate (18.73%), 1- dotriacontanol (15.35%), 5- eicosene, (E)- (11.90%). The compounds were found to be dissimilar in both leaf and flower. Quantitative phytochemical analysis of leaf extract was found to contain a high concentration of phenols, flavonoids and saponins, whereas total alkaloids were maximum in flower extract. In in-vitro, the antioxidant activity was evaluated using 1, 1-diphenyl-2- picrylhydrazyl (DPPH) assay. Both leaf and flower extract showed remarkable antioxidant scavenging activity with an...
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Posted by admin on Sep 30, 2020 in |
Objective: The aim of this study was to screen various solvent extracts of leaves of Rumex hastatus to display in-vitro antioxidant activity, total phenolic, and flavonoid contents in order to find possible sources for future novel antioxidants in food and pharmaceutical formulations. Materials and Methods: A detailed study was performed on antioxidant activity of various Rumex hastatus leaves extracts like petroleum extract (PERH), chloroform extract (CERH), ethyl acetate extract (EAERH), methanolic extract (MERH) and aqueous extract (AERH) by in-vitro chemical analysis like DPPH, Reducing power, metal chelating, hydrogen peroxide and nitric oxide free radical scavenging assay. All these extracts were also subjected to a preliminary phytochemical screening test for various constituents. The total yield, total phenolic, and total flavonoid contents of all extracts were also determined. Results: Phytochemical screening shows the presence of triterpene, flavonoids, polysaccharides, steroids, tannins, cardiac glycoside, reducing sugar, and saponins in all extracts. The MERH exhibited the highest total phenolic content (121.7 ± 5.95 mg of GAE/g extract) and total flavonoid content (165.9 ± 3.99...
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Posted by admin on Sep 30, 2020 in |
A Simple and trace level-sensitive Gas chromatography with mass spectrometer (GCMS) method was developed, optimized and validated for the determination of seven potentially genotoxic impurities which are having structural alerts i.e., 1-Bromo-5-chloropentane (PGI-1), Ethyl-6-chloro-2-oxo hexanoate (PGI-2), Methyl-7-chloro-2-oxo hepta-noate (PGI-3), Ethyl-7-chloro-2-oxo heptanoate (PGI-4), Ethyl-7-bromo-2-oxo heptanoate (PGI-5), Ethyl-8-chloro-2-oxo octanoate (PGI-6), Ethyl-2-acetyl-7-chloro heptanoate (PGI-7) contents in Cilastatin sodium drug substance. The chromatographic separations of seven genotoxic impurities were achieved on ZB-5 MS [5% Polysilarylene, 95% Poly-dimethylsiloxane copolymer capillary column of 30 m length, 0.32 mm internal diameter, film thickness 1.0 µm]. The analytic method has been demonstrated through method validation experiments w.r.t ICH guidelines, like specificity, linearity, Limit of Detection, Limit of Quantification, method precision and accuracy (recovery) parameters with allowable TTC limit. The achieved limit of detection (LOD) values was 3 µg/g. This developed method was found to be linear with correlation coefficient is greater than 0.999. Developmental and validation experiments were discussed in detail in this...
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