Posted by admin on Jan 31, 2025 in |
Sargassum wightii,a seaweed and a marine macroalgae, is known to offer numerous health benefits, and compounds isolated from it are observed as the alternative to conventional treatment as an affordable plant-derived drug. In the present study, the bioactivity of the two fractions from Sargassum wightiiw as derived using two different solvents, methanol and acetone: methanol. Acetone: Methanol fraction showed better yield. Both the fractions it was tested against both gram-positive Staphylococcus aureus and gram-negative Escherichia coli bacteria. The antioxidant potential was evaluated by the reducing power assay. Fucoxanthin extracted by maceration in methanol: acetone (9:1) showed more excellent antioxidant activity. The partially purified extract was also analysed through the FT-IR spectra. The extract prepared by methanol: Acetone extract performed better than streptomycin, but compared to streptomycin, none of the extracts showed significant antibacterial action at higher concentrations. The FT-IR spectra showed various secondary metabolites and their derivatives in the crude...
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Posted by admin on Jan 31, 2025 in |
The present work focused on preparing ethanolic extract of Petunia hybrida and evaluating its anti-diabetic potential in rats. The extraction of flowers was carried out using ethanol and the extraction yield was found to be 21.7% w/w. The total phenolic content of ethanolic extract of Petunia hybrida was found to be 39.18±1.46 GAE mg/g. The antidiabetic action of the extract was evaluated in terms of the effect of extract on body weight and serum glucose level. Other parameters like cholesterol and serum urea were also evaluated. The results revealed that the serum glucose levels were highly elevated by the administration of STZ in all the groups by day 7. In treatment groups III to V, the glucose levels dropped in comparison to group II and by the day 21, the levels reached almost the level of control group in group III and V. This indicates that the extract exhibits significant glucose lowering potential. Both SGOT and SGPT enzyme levels get elevated during liver damage which is more in diabetic...
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Posted by admin on Jan 31, 2025 in |
Introduction: Infections due to methicillin resistant Staphylococcus aureus (MRSA) are increasing worldwide. Vancomycin is considered as the drug of choice to treat serious infections caused by MRSA strains. The emergence of S. aureus isolates with intermediate and complete resistance to vancomycin in the past two decades is a major public health concern. Aim and Objectives: To determine the MIC of vancomycin in MRSA isolates from various clinical specimens and to observe the occurrence of VRSA and VISA. Materials and Methods: This cross-sectional study was conducted between November 2020 to October 2021 in the department of Microbiology, RMCH, Bareilly. S. aureus isolates were identified using routine identification methods. MRSA isolates were subjected to E-test for detection of MIC of vancomycin through vancomycin Ezy MIC Strips (Hi Media Range .016-256μg/ml). Results: A total of 180 S. aureus strains were isolated during the study period. Out of these strains 100 (55.55%) MRSA and 80 (44.45%) MSSA were isolated. Out of 100 MRSA strains 8 strains showed vancomycin MIC of 0.75 μg/ml, 50 strains...
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Posted by admin on Jan 31, 2025 in |
Sulfadiazine belongs to a class of sulphonamide antibiotics. Sulfadiazine is a synthetic bacteriostatic sulphonamide that exhibits a broad spectrum of activity against gram-positive and gram-negative pathogens. The Sulfadiazine antibiotic is a member of the Sulfa drugs class. This drug is utilized for bacterial infections in the brain, ears, and urinary tract. The primary goal of the research is to develop and validate Sulfadiazine by Reverse Phase (RP) HPLC. An HPLC (Alliance, Water 2695) with a PDA detector and Avantor -ACE C18 250 mm × 4.6 mm × 5 µm column (Part Number ACE 121-2546) was used. A novel technique for the simultaneous measurement of sulfadiazine using the RP-HPLC technology was developed. The ACE C18 column (250×4.6 mm) 5µm successfully created the chromatographic conditions needed to separate sulfadiazine. The flow rate was 1 mL/min, and the mobile phase ratio of ACN: H2O was 70:30 v/v. 254 nm was the detection...
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Posted by admin on Jan 31, 2025 in |
Background: Residual cardiovascular risk persists despite optimal LDL-C lowering, suggesting that remnant cholesterol (RC) may contribute to atherosclerosis. Objective: To evaluate the association between RC, lipid profile discordance, and cIMT, and determine if RC and lipid discordance are independent predictors of increased cIMT after adjusting for traditional cardiovascular risk factors. Methods: In this cross-sectional study of 60 patients (mean age 56 ± 10 years, 63% male) with cardiovascular conditions, cIMT measurements and lipid profiles were obtained. RC was calculated as total cholesterol minus LDL-C minus HDL-C. Patients were grouped into four categories based on median LDL-C and RC levels. Statistical analyses assessed associations between RC, lipid discordance, cIMT, and cardiovascular risk factors. Results: RC showed a moderate positive correlation with cubed mean cIMT (r = 0.36, p = 0.0047). Patients with high discordant RC had significantly higher cubed mean cIMT compared to those with low concordant levels. In regression analysis, body mass index (β = 0.337, p < 0.001), smoking status (β = 0.819, p = 0.002), and LDL-C (β = 0.010, p = 0.044) were significant predictors of cubed cIMT, while RC showed a trend toward...
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