Posted by admin on Aug 31, 2024 in |
Health care associated infections (HAIs) are frequently caused by Klebsiella pneumoniae (K. pneumoniae) which has a high level of antibiotic resistance pattern and the ability to form biofilms. In this research work, 105 K. pneumoniae was isolated from both inpatients and outpatients between January 2021 and January 2022 at the tertiary care hospital viz, Mahatma Gandhi Medical College and Research Institute (MGMCRI), India. The K. pneumoniae isolates were further analysed using morphological analysis of the colonies, microscopic examination, and biochemical testing. The Kirby Bauer disk diffusion method and adhesion quantitative assays were utilized for testing antibiotic susceptibility and biofilm- producing capacity. K. pneumoniae isolates were mostly derived from the urine specimens (42.9%) and pus (19.0%). Most of the K. pneumoniae were resistant to a wide range of antibiotics and is also well known to the ability to produce biofilm. This study shows that the biofilm producing K. pneumoniae has been a good resistant to Ceftriaxone/Cefotaxime (80.9%), Amikacin (78.7%), Piperacillin+ Tazobactam (79.8%), and Meropenem (79.8%). On the contrary among non-...
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Posted by admin on Aug 31, 2024 in |
Nowadays increase in UV induced skin ailments and adverse effects of synthetic UV filters, prompted researchers to focus on natural compounds capable of absorbing UV rays. Garcinia indica is a rich source of benzophenones, which are present in all plant parts. As per earlier reports, ethyl acetate root extract of it exhibits the highest SPF (7) than other plant parts. The present study aims to characterise anti-UV benzophenones and screening of in-vitro roots for it. Natural root extract subjected to silica gel column chromatography. The column fractions were analysed using a spectrophotometer and thin-layer chromatography. Initial fractions eluted in hexane to hexane: ethyl acetate 90:10 or 80:20 displayed a fluorescent blue band on the TLC plate and showed absorbance in the UV B region. As the concentration of ethyl acetate increased (Hexane: Ethyl acetate 70: 30) the eluted fractions exhibited an additional fluorescent green band. These fractions had a broad-spectrum UV absorption activity contributing to SPF and Boot star ratings. HPTLC-MS analysis of these two bands characterized garcinol (m/z...
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Posted by admin on Aug 31, 2024 in |
Guibourtia ehie (A. Chev.) J. Leonard is a medicinal plant widely used in the Ivorian traditional medicine against stomach ulcers, sexual infections, diabetes, hypertension and microbial infections, particularly those caused by amoeba and fungi. Furthermore, this plant is widely known for its commercial interest due to economic value of its wood. Biological and phytochemical investigations also confirmed local medicinal uses of the plant and showed a various chemical composition including numerous polyphenols. In this article, we reported the results of leaves studies of Guibourtia ehie, harvested in Côte d’Ivoire, which resulted in the isolation of a new flavonol, 8-isopentenylkaempferol-7-O–β-D-(2’’’-O-galloyl) glucopyranoside (1), that we named 2’’-O-Galloyl-epimedoside C, along with two phenolic known compounds, orcinol (2) and methyl orsellinate (3). The structures of compounds where elucidated by spectroscopic data including HR-ESIMS, UV, NMR 1D (1H and 13C) and NMR 2D (COSY, HSQC and HMBC). These compounds were isolated for the first time from Guibourtia ehie (A. Chev.) J....
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Posted by admin on Aug 31, 2024 in |
A fast, efficient and defined method was developed for the real-time assessment of Sitagliptin in tablet dosage form. Dissolution method was developed and carried out to predict in-vivo drug release profiles of Sitagliptin tablets. Rate and extent of dissolution of Sitagliptin tablets was studied in different dissolution medias i.e. 0.1 N HCL, pH 4.0 Acetate buffer and pH 6.8 Phosphate buffer using USP Type 1 dissolution apparatus with a RPM of 100. Ultra performance liquid chromatography was performed using BEH C18 Column (2.1x 50mm, 1.7µm). Moving phase containing Phosphate Buffer of pH 7.2, Acetonitrile and Methanol in ratio 60:20:20v/v/v was pumped through the column at 1 mL/min flow rate. Run time is 3 minutes. Injection volume is 1.7μL and column is maintained at a temperature of 25°C. Selected wavelength is 210 nm. The Dissolution method was developed and validated and was found to be Precise, Accurate, Linear in a Concentration Range of 25 % to 175 % of Test concentration. Sitagliptin retention time was found to be 1.592 minutes....
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Posted by admin on Aug 31, 2024 in |
Medicinal plants are important for pharmacological research and drug development. Aim and Objective: The aim and objectives of present study was to evaluate the wound healing activity of seed oil of Azadirachta indica in Excision and Incision wound models in rats. Result Discussion: The study thus demonstrated the wound healing activity of Neem seed oil and found to be effective in the functional recovery of the wound. It promotes wound contraction of excision wound as compared to control...
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