Posted by admin on Sep 30, 2020 in |
The overproduction of oxidants in the body leading to different side effects and chronic diseases, including oxidative stress. The oxidative stress is the overproduction of the oxidant’s condition in the body, which leads to metabolic imbalance and leading to diseases. It is one of the main diseases around the world, affecting the human lifestyle, either psychologically and physically. The natural products have been using as medicines and food supplements because of their nutritional values and less side effects. The current study was aimed to evaluate the free radical scavenging activity of Elytraria acaulis root extracts on different free radicals. The anti-oxidant activity was studied against 2, 2-diphenyl-1-picrylhydrazyl (DPPH), superoxide, and hydroxyl free radicals using standard methods. The Elyraria acaulis root part extracts showed concentration-dependent antioxidant activity on free radicals. The extracts showed a moderate effect on free radicals compared to the standard drug ascorbic acid, and hydro-alcoholic extract possesses more activity than the other two extracts. The end results of the current study clearly state that Elytraria acaulis root...
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Posted by admin on Aug 31, 2020 in |
The objective was to develop and validate an easy, economical, fast, reliable, reproducible, precise and accurate reversed-phase high-performance liquid chromatography (RP-HPLC) method for the estimation of tapentadol hydrochloride (TAP, a mu opioid-receptor agonist, and noradrenaline reuptake inhibitor) in the bulk and pharmaceutical dosage form. The chromatographic separation was achieved by using the HPLC system equipped with a C-18(2) column (250 × 4.6 mm, Particle size 5µm) at wavelength 217 nm. The mobile phase consisting of Potassium phosphate buffer: Acetonitrile (50:50 v/v) was used in isocratic mode. The flow rate was fixed at 1.0 mL/min with a continuous run up to 5 min, while the retention time was located near about 2.5 min. In the concentration range of 60-160 µgmL-1, the detector response was found linear with linear regressed equation Y=22791X+120122. In the assay of TAP, 99.65% of the drug was recovered. This method proved a satisfactory validation for all the parameters such as accuracy, linearity, specificity, precision, range, ruggedness, robustness, reproducibility, and peak purity assessment as per ICH guidelines. The results...
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Posted by admin on Aug 31, 2020 in |
Kutki (Picrorrhiza kurroa) is a medicinal herb with remarkable pharmacological properties. However, poor solubility of the active principles limits its medicinal value. This study sought to prepare kutki phospholipid complex in phospholipids such as phosphatidyl choline in order to improve its solubility and permeability. Kutki phospholipid complex (phytosome) was prepared by solvent evaporation method. The study was conducted using central composite design (CCD) with a 33 designs and total of 20 experimental runs. The complex was characterized for solubility, entrapment efficiency, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), particle size and zeta potential, Differential scanning calorimetry (DSC), X-ray diffraction (XRD), drug release, and in-vitro antioxidant activity. The optimized batch showed substantial enhancement of solubility and entrapment efficiency. FTIR study indicates the interaction between phosphatidylcholine and Kutki extract. SEM showed the surface morphology of complex depicting the conversion of crystalline drug into amorphous complex. The in-vitro release was significantly higher for the complex (93.43%) than the drug (19.65%) after 7 h. Enhanced solubility, absorption and antioxidant effect may...
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Posted by admin on Aug 31, 2020 in |
A simple and rapid reverse-phase high-performance liquid chromatographic technique was developed and validated for the determination of Luliconazole in the presence of its excipients. Chromatographic elution was performed on a binary gradient HPLC equipped with PDA detector using a Luna-5µ C8(2) 100 Å column (250 × 4.6 mm, 5 µm) with orthophosphoric acid (0.1%) and methanol (20:80% w/v) as mobile phase at a flow rate of 1.2 mL/min. The method follows Beer-Lambert’s law over a concentration range 0.1-200 μg/mL (y = 53981x + 25076, R2 = 0.999). The LOD and LOQ were found to be 0.068 µg/mL and 0.206 µg/mL indicating the sensitivity of the method with the required precision and accuracy. The method proved to be specific as there was no interference from the commonly used excipients in a formulation like methylparaben and also in the presence of degradants when exposed to a variety of stress conditions (acidic, alkaline, oxidation, thermal, hydrolytic and photolytic degradations). It was observed that Luliconazole was more sensitive towards alkaline conditions, and hence...
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Posted by admin on Aug 31, 2020 in |
Asthma is a chronic disease; there is a need for a drug delivery system that maintains adequate therapeutic concentrations for a longer duration of action to improve better clinical efficacy. Salbutamol sulphate (SS) is a widely prescribed drug to treat asthma though it has short plasma half-life and undergoes extensive first-pass metabolism. Thus, it necessitates frequent administration by oral route to maintain adequate plasma concentrations. Hence an attempt was made to formulate and evaluate transdermal ethosomal gel formulations of SS to improve therapeutic efficiency and to reduce the dosage regimen. Ethosomal suspensions of drugs were prepared using phosphatidylcholine, alcohol, propylene glycol, and cholesterol. Then, suspensions were used to develop gels by incorporating them into carbopol. Formulations were evaluated for vesicular size, size distribution, scanning electron microscopy, drug content estimation, drug entrapment efficiency, in-vitro diffusion studies, Z-Average, polydispersity index (PDI), and zeta potential measurement. Analytical studies confirmed the absence of drug and polymer interaction. Drug content of ethosomal suspensions and gel formulations was found uniform, and microscopic images displayed that...
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