Posted by admin on Oct 31, 2020 in |
Background: Majoon hajrul yahood is a polyherbomineral formulation commonly used in the Unani system of medicine for the management of urolithiasis. In this study safety of a hydroalcoholic extract of majoon hajrul yahood was evaluate in Wistar rats. Materials and Methods: The hydroalcoholic extract of the formulation were tested for acute and sub-acute toxicity study. The single-dose (300, 2000, and 5000 mg/kg) of the extract was administered orally to female rats. In the subacute study, the extract was given at doses of 1/5, 1/10, and 1/15 mg/kg (1000, 500, and 333.33 mg/kg) of maximum dose (5000 mg/kg) of acute toxicity study during 28 days orally. Mortality, changes in body weight and food consumption, organ weights, biochemical, hematological, and histopathology of the organs were performed. Results: In the acute study, the extract was classified as safe, according to the OECD guide in category 5. In the present study, results revealed that subacute treatment with test extract, there is no changes in biochemical, hematological, and histological changes in the tissues. Conclusion:...
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Posted by admin on Oct 31, 2020 in |
Amla, Arjuna, Gudmar, Guduchi, and Ginger have been usually used in the traditional system of medicine for the treatment of diabetes. The aim of the present study to evaluate antihyperglycemic activity of polyherbal and allopolyherbal formulation on Streptozotocin-Nicotinamide (STZ-NA) induced type-2 diabetes rats. All the extracts of herbs used for the present study were obtained by supercritical fluid extraction. The Oral glucose tolerance test (OGTT) and anti-hyperglycemic activities were performed on STZ-NA-induced diabetic rats. The effects of metformin, PHF, and allopolyherbal formulation (APHF) on plasma insulin, hemoglobin, liver glycogen, and glycated hemoglobin (HbA1c) were analyzed. Statistical analysis was done by using one-way ANOVA followed by Dunnett’s multiple comparison tests. The OGTT of metformin, PHF, and APHF showed increased glucose tolerance. Single-dose anti-hyperglycemic activity of metformin, PHF, and APHF showed a significant (P<0.01) decrease in blood glucose level (BGL) in diabetic rats at 1st, 3rd, 5th, and 7th h. Multiple doses anti-hyperglycemic activity of metformin, PHF, and APHF showed significantly (P<0.01) reduction of BGL in diabetic rats after 14th, 21st,...
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Posted by admin on Oct 31, 2020 in |
Background: Serum total bilirubin (SBT) and serum uric acid (SUA) have anti-oxidant properties. SBT and SUA may be potential biomarkers for type 2 diabetes mellitus (T2DM) and its complications. Aim: It is aimed to evaluate the level of STB and SUA in T2DM. Materials and Methods: In this case-control study, a total of 124 subjects (62 T2DM cases and 62 age and gender-matched controls) were recruited between the age group of 30-55 years. Fasting blood sugar (FBS), STB, SUA, Malondialdehyde (MDA), and Superoxide dismutase (SOD) were estimated. All the data were tested at a 5% level of significance. Results: The mean level of SUA was significantly higher in cases than controls (P=0.009). However, the mean level of STB was not found statistically significant in cases than controls (P=0.08). The mean level of MDA was found significantly elevated in cases than controls (P<0.001). In addition, the mean of SOD activity was found significantly low in cases than controls (P<0.001). A significant negative correlation was found between age and SOD activity...
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Posted by admin on Oct 31, 2020 in |
A series of 2-cyclopropylimidazo [2,1-b] [1,3,4] thiadiazole derivatives 5(a-i) have been synthesized by reacting 5-cyclopropyl-1,3,4-thiadiazol-2-amine (3) and an appropriate 2-bromo-1,2-(substituted aryl) ethanones 4(a-i). Structures of these compounds were recognized by IR, 1H NMR, 13C NMR spectroscopy and Mass spectrometry. Hypoxia-inducible factor (HIF) has been identified as an important cancer drug target. HIF transcription complex, which is activated by low oxygen tension, controls a diverse range of cellular processes, including angiogenesis and erythropoiesis. Here we analyzed the capacity of synthesized molecules to inhibit hypoxia-inducible factor prolyl hydroxylase (PHD2) in-silico as well as an in-vitro assay. Four compounds were granted NSC code at National Cancer Institute (NCI), USA, for anticancer activity at a single high dose (10-5 M) in full NCI 60 cell panel. Among the compounds tested,2-Cyclopropyl-6-(4-methoxyphenyl)-5-phenylimidazo[2,1-b][1,3,4]thiadiazole 5a (NSC D-754956/1) was found to be the most active candidate of the series at five dose level screening with a degree of selectivity toward leukemic cancer cell...
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Posted by admin on Oct 31, 2020 in |
The aim of the study was to determine the biologically active fractions of methanol extracts of mature leaves of Pogostemon benghalensis (burm. f.) Kuntze and Perilla frutescens L. Britton. The methanol extract was broadly fractionated sequentially in hexane, chloroform, acetone, ethyl acetate, and methanol. Activity index was recorded higher in methanol broad fractions of both of the plants. Broad fractions of both of the plants recorded a higher Proportion Index against E. coli (1.00). An increasing trend was recorded in the case of total phenol and flavonoid content with an increase in concentration from 1 mg/ml to 5 mg/ml. In case of Pogostemon benghalensis (Burm.f.) Kuntze, antioxidant inhibition of ascorbic acid against DPPH and ABTS are (98.28 ± 1.03% and 96.22 ± 2.01% respectively) which is higher than the crude extract (81.43 ± 0.09% and 72.32 ± 0.01% respectively) at 500 µl of the sample at 1mg/ml of concentration. TLC Spots from hexane and chloroform broad fraction recorded antibacterial inhibition against B. subtilis. Rf values of TLC spots from...
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