Posted by admin on Jul 31, 2020 in |
The application of nanobiotechnology is an emerging area of nanoscience and nanotechnology. In the present studies, tungsten nanoparticles were synthesized and reduced chemically, and character-rization was done by UV, SEM, TEM, FT-IR, and XRD. The size of nanoparticles was found to be 20 nm. Further, these nanoparticles were teste for various biological applications. In antimicrobial activity, it was observed that potent efficacy was observed against Streptomyces grisveus (12 mm) at 80 µg/ml while in fungus maximum activity was observed against Penicillium funicolusum (24 mm) at the same dose. The antiplatelet activity of nanoparticles was investigated by Prothrombin (PT) and Activated Partial Thromboplastin Time (APTT). In both PT and APTT assay, maximum activity was observed at 40 µg/ml (295 sec and 80 sec, respectively). Cytotoxicity was also studied by MTT assay against various cell lines. Against MCF-7, potent activity was observed at 200 µg/ml, while in 3T3 it was observed at 500 µg/ml. Finally, it was observed that these nanoparticles have potent activity against tuberculosis at 1000 µg/ml. The result...
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Posted by admin on Jul 31, 2020 in |
The present study was aimed to evaluate the in-vitro anti-inflammatory activity of fruits and leaves extract of Cucumis melo L. subsp. agrestis (Naudin) Pangalo (Family Cucurbitaceae). The ethanol and aqueous extracts of fruits and leaves of the plant were subjected to preliminary phytochemical screening and in-vitro anti-inflammatory activity. Extracts were incubated with bovine albumin under controlled conditions for denaturation, and protein denaturation was calculated by determination of their absorbance. Since, the erythrocyte membrane is analogous to the lysosomal membranes and its stabilization implies that the extract may well stabilize lysosomal membranes thus human red blood cell membrane stabilization was used as a method to study the in-vitro anti-inflammatory activity. The results showed that the plant extracts showed anti-inflammatory activity in a concentration-dependent manner, and the activity was increased on increasing the concentration of extracts. The ethanol extract was found to be more effective than other extracts of fruit and leaf. The present study reveals that the fruit of Cucumis melo L. subsp. agrestis (Naudin) Pangalo possesses a higher anti-inflammatory...
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Posted by admin on Jul 31, 2020 in |
In the present study, we had developed Asenapine maleate (ASNM) loaded microemulsion (ME) to increase the solubility of Asenapine maleate by components of microemulsion including formulation concern, characterization, mucosal diffusion, stability and nasal ciliotoxicity of Asenapine maleate microemulsion (AME). For nasal delivery of Asenapine maleate, a challenge existing in formulation development is the solubilization of poorly water-soluble Asenapine maleate. The intrinsic solubility of Asenapine maleate is about 0.0312 mg/ml. The purpose of this study was to improve the solubility and to enhance the brain uptake of Asenapine maleate through an o/w microemulsion, with suitable intranasal delivery. The optimal microemulsion formulation consisted of Oleic acid, Tween 80: Propylene glycol (PG) (3:1) and water, with a maximum solubility of Asenapine maleate and no ciliotoxicity, was developed and characterized. AME was characterized for % Transmittance, pH, viscosity, globule size, zeta potential, drug content with in-vitro release studies and Ex-vivo diffusion studies using Standard Franz Diffusion cell. Further, the behavioural studies were assessed in rats by inducing hyperactivity. The optimized microemulsion was found...
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Posted by admin on Jul 31, 2020 in |
The purpose of this work was to develop prolonged-release solid lipid nanoparticles (SLNs) of Rifampicin (RIF) for oral drug delivery and to improve the bioavailability of RIF. SLNs were designed by using Glycerol monostearate (GMS) and lipid core materials and polaxamer 188 as a stabilizer. SLNs were prepared by o/w microemulsion technique and characterized by particle size analysis, Fourier transforms infrared (FTIR) spectroscopy, differential scanning calorimetry (DSC), drug entrapment efficiency, scanning electron microscopy (SEM), Zeta potential, in-vitro evaluation studies, storage stability, membrane permeation study. At the highest speed, the resultant SLNs were smaller in size, and their size increased with an increase in lipid concentration. Nanoparticles prepared using 2.5% lipid, 1% Poloxamer 188, and 21,500 rpm show smaller particle size, better drug entrapment, excellent surface characteristic, and controlled drug release. Release from RIF-SLNs was studied using a dialysis bag method. The results of the released in-vitro study show that the dialysis membrane after 12 and after 24 h, the release pattern of drug slightly increases. The surface characters were...
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Posted by admin on Jul 31, 2020 in |
The anthelmintic effect of alcoholic Citrullus colocynthis fruit extract on the tegument of Cotylophoron cotylophorum was studied by light microscopy. Live amphistome Cotylophoron cotylophorum flukes were collected from the infected rumen of freshly slaughtered domestic buffaloes (Bubalus bubalis) at the local zoo abattoir in Udaipur. The control and anthelmintic treated C. cotylophorum with the alcoholic C. colocynthis fruit extract, and flukicide drug albendazole were fixed in Bouin’s fixative for histological study. The present studies revealed the alcoholic Citrullus colocynthis fruits extract effect on the tegument of flukes; showed abundant cuticular disruption, detachments, discontinuous, blebbing, clumping of parenchymal cell and the complete breakdown of cells in the parenchyma leaving vacuolated areas. Albendazole is less effective than alcoholic Citrullus colocynthis fruit extract. This study suggests that the alcoholic extract of the fruit of C. colocynthis could offer a suitable and cheaper alternative anthelmintic as a comparison to synthetic drugs. The results of this study will help to prepare the eco-friendly, less costly anthelmintic veterinary drug and socio-economic upliftment of cattle...
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