Posted by admin on Jul 31, 2020 in |
Seeds of Macrotyloma uniflorum Linn. (Fabaceae) are widely used for treatment in kidney stones in India. In This study evaluates the in-vitro effect of methanolic extract of Macrotyloma uniflorum (MEMU) on calcium oxalate crystallization. A methanolic extract of M. uniflorum seeds at different concentrations (250-1500 μg/ml) was subjected to in-vitro crystallization activity. The obtained results were compared with a Cystone (standard herbal product) available in the market. The nucleation and crystal growth assay was performed with different concentrations of sodium oxalate (2-10 mmol/ml for nucleation, 2-3.5 mmol/ml for crystal growth) and evaluated the effect of MEMU and cystone at different doses (250-1500 μg/ml) on the different concentration level of oxalate. Crystal aggregation and crystal dissolution assay were performed and compared with cystone effect. The MEMU was significantly more effective than cystone at inhibiting the nucleation rate and crystal growth at a different concentration level of oxalate. Inhibition of calcium oxalate crystal aggregation and dissolution was observed in a dose-dependent manner. The results showed that MEMU has excellent in-vitro calcium...
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Posted by admin on Jul 31, 2020 in |
The present investigation aimed at the study of resistant plasmid patterns of multidrug-resistant Escherichia coli and Pseudomonas aeruginosa isolated from hospital-acquired infection in urinary tract site are causes serious public health hazard throughout the world. A total of 100 urine samples were collected from patient admitted to the hospital. The antibiotic sensitivity of isolated uropathogens was performed by Kirby- Bauer method. The plasmid profiles of multidrug-resistant isolates were extracted by the alkaline lysis method. The multiple antibiotic-resistant indexes of uropathogens were calculated and phylogenetically determined. Among 100, 23 urine samples were positively isolated and identified. The Escherichia coli and was found to be predominantly followed by Pseudomonas aeruginosa. The multiple antibiotics resistant index was calculated as 0.75 and 0.464 respectively for Escherichia coli HAUTI8, and Pseudomonas aeruginosa HAUTI18, and these strains were phylogenetically identified. The Plasmid profile of Escherichia coli and Pseudomonas aeruginosa indicates wide ranges of plasmid among the resistant strains between 23,130 to 2027 base pairs. As a result, multidrug-resistant was an emerging disseminated clinical problem rapidly...
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Posted by admin on Jul 31, 2020 in |
In the healthcare system, the development and modernization of phytomedicine in phytochemistry play a central and important role in drug development. Isolation and purification of bioactive compounds from naturally occurring substance are of prime importance. A sensitive and reliable high-performance thin-layer chromatography method has been developed for the estimation of gallic acid in the alcoholic extract of Eulophia ochreata Lindl. Alcoholic extract prepared was applied on silica gel G 60 F254 plate. The plate was developed using toluene: ethyl acetate: methanol: formic acid (6:6:0.4:1.6) as a mobile phase detection and quantification were performed by densitometric scanning at 275 nm. The system was found to give well-resolved bands for alcoholic extract having Rf values as 0.8 was matched with the standard Rf values like 0.82. The densitometric chromatogram of HPTLC fingerprint of the alcoholic extract, isolate, and standard gallic acid was obtained. The calibration curve of gallic acid was linear over a concentration range (0.2- 2 microg/ml) with a good correlation coefficient (R2= 0.9986) and coefficient of variation as CV-...
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Posted by admin on Jul 31, 2020 in |
The compounds that are derived from the natural sources have been used for the treatment of cancer since ancient times. The tubers of Eulophia nuda, belonging to family Orchidaceae, have been reported for its anti-cancer use in the literature. There are many disadvantages to the conventional herbal drug delivery system. These disadvantages could be overcome by formulating these herbs using novel drug delivery systems. Microemulsions are the novel formulations that improve the solubility and bioavailability of herbal bioactive bio-active compounds. In the present study, microemulsion loaded with alcoholic extract of tubers of E. nuda has been formulated by the water titration method using oil (Oleic acid), surfactant: cosurfactant (Smix) (Tween 80 and PEG400) and water. The prepared formulations of microemulsions were subjected physicochemical characterization parameters such as globule size, percentage transmission, refractive index, viscosity, pH, drug loading, and percentage drug permeated. The optimized microemulsion was further subjected to an in-vitro cytotoxicity study by MTT assay using the MCF7 breast cancer cell line. The optimized microemulsion loaded with alcoholic extract...
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Posted by admin on Jul 31, 2020 in |
Notable progress has been made in methods that encourage the use of PCR as a rapid and accurate tool in quality evaluation of pharmaceuticals. In this study, monoplex and multiplex PCR based assays were developed and compared with standard conventional methods for rapid detection of three specified topical indicator pathogens, Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans, in nonsterile pharmaceutical preparations. The detection limit of monoplex PCR assays for the microbial targets was achieved at 100 fg purified DNA and 10 CFU/ml for Pseudomonas aeruginosa and 1fg purified DNA and 10 CFU/ml for each of Staphylococcus aureus and Candida albicans. No change in the detection limit for cfu/ml of the three tested indicator pathogens was obtained upon using mPCR assays. The results of applying both conventional and PCR detection methods for different cream and lotion preparations revealed a 100% correlation between both methods. The PCR based detection method can be completed in 8 h versus 5-6 days in case of conventional methods, but the former can’t differentiate between viable...
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