Posted by admin on Jul 31, 2020 in |
Liver fibrosis is a wound healing process that is initiated in response to chronic liver injury caused by viruses, toxins, and hepatotoxic drugs. This disease is characterized by inflammation, followed by the deposition of extracellular matrix proteins to form scar tissue. Ephrin receptor A2 (EphA2) is identified as a host cofactor for Hepatitis C Virus (HCV) entry. The fruits of Solanum nigrum possess anti-inflammatory properties. The present work was done to evaluate the anti-inflammatory activity of the ethanolic fruit extract of Solanum nigrum against EphA2 receptor using Induced fit docking of Schrödinger software. Phyto-compounds were retrieved from the PubChem database. The 3D structure of EphA2 was downloaded from Protein Data Bank (PDB). Among the five phytocompounds, Ethyl caffeate has the Glide Score of -8.01 and glide energy-35.48 kcal/mol. From the results, it is evident that the phytoconstituents of Solanum nigrum fruits have anti-inflammatory potential and prevent hepatic fibrosis. Molecular properties of the compounds were predicted using the Molinspiration tool, and the pharmaco-kinetic properties of the compounds were predicted using...
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Posted by admin on Jul 31, 2020 in |
The current study work is focused on Dapoxetine hydrochloride buccal films. In recent days, buccal drug delivery has aimed at importance in many aspects compared to conventional tablets. The addition of mucoadhesive polymers to the formulation enhances the therapeutic levels of drug. Dapoxetine hydrochloride, a choice of drug used in the therapy of premature ejaculation in men. Dapoxetine hydrochloride is a selective serotonin reuptake inhibitor (SSRI’s) whose oral bioavailability is 42% due to hepatic first-pass metabolism. To enhance the bio-availability and drug release, Dapoxetine hydrochloride is designed as buccal films. They are prepared by the most commonly used solvent-casting method. Two grades of Hypromellose (E15 and 5cps), polyvinyl alcohol, and polyvinyl pyrrolidine are polymers that are mucoadhesive in nature. Propylene glycol is used as a plasticizer, and also mucoadhesive polymer and methanol as a solvent are used in film preparation. FTIR studies were done, and there is no incompatibility between active pharmaceutical ingredient (API) and excipients. The formulations developed were evaluated for different parameters such as weight uniformity, thickness,...
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Posted by admin on Jul 31, 2020 in |
This research explores Achyranthes bidentata L., Adhatoda zeylanica and Aeginetia indica L’s pharmacological potential. Antibacterial disc assay (20 mg/ml), antifungal disc assay (20 mg/ml) and antioxidant DPPH/reducing energy assay (50 μg/ml, 100 μg/ml and 200 μg/ml) were tested for biological operations of plant extracts (aqueous, methanol and acetone leaf extracts). Significant alkaloid content (378.37 ± 35.02 mg/g) was identified in Aeginetia indica L. with the lowest tannin content in Achyranthes bidentata L. (13.37 ± 3.15 mg/mg). Aeginetia indica L. methanol extract (99.28 ± 10.47%) disclosed significant antioxidant potential (p<0.05). Significant antibacterial and antifungal activity was demonstrated against Escherichia coli (22.80 ± 5.06 mm), Pseudomonas aeruginosa (22.80 ± 5.06 mm), Bacillus subtilis (25.40 ± 5.21 mm), Candida albicans (19.40 ± 2.05 mm) and Aspergillus niger (19.80 ± 1.48 mm) from Aeginetia indica L. methanol extracts. All plant methanol extracts showed significant antibacterial activity (22.80 ± 5.06 mm – 25.40 ± 5.21 mm) and antifungal activity (19.40 ± 2.05 mm – 19.80 ± 1.48 mm) very close to standard medicines whereas...
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Posted by admin on Jul 31, 2020 in |
In the study, we have successfully synthesized N-3 substituted tetrahydroquinazolinone by the reaction of isotonic anhydride with aniline derivatives. Thereafter novel derivative would be subjected to synthesize (1, 2, 3, 4-tetrahydroquinazoline) by Mannich reaction with ciprofloxacin and norfloxacin. These synthesized compounds (2a, 2b, 2c, 2d, 2e, 2f, 2g, and 2h) were characterized by FT-IR, 1H NMR, MASS spectroscopy, and elemental analysis and evaluated for their in-vitro antimicrobial activity and anti-inflammatory activity. The antimicrobial activities of synthesized compounds were evaluated against gram-positive and gram-negative bacteria. Amongst these tested compounds 2c, 2d, 2e, 2f exhibited potent antimicrobial activity than the rest of the compounds. All the synthesized compounds were screened for their anti-inflammatory activity using the carrageenan-induced rat paw edema model and exhibited protection against carrageenan-induced...
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Posted by admin on Jul 31, 2020 in |
The application of nanobiotechnology is an emerging area of nanoscience and nanotechnology. In the present studies, tungsten nanoparticles were synthesized and reduced chemically, and character-rization was done by UV, SEM, TEM, FT-IR, and XRD. The size of nanoparticles was found to be 20 nm. Further, these nanoparticles were teste for various biological applications. In antimicrobial activity, it was observed that potent efficacy was observed against Streptomyces grisveus (12 mm) at 80 µg/ml while in fungus maximum activity was observed against Penicillium funicolusum (24 mm) at the same dose. The antiplatelet activity of nanoparticles was investigated by Prothrombin (PT) and Activated Partial Thromboplastin Time (APTT). In both PT and APTT assay, maximum activity was observed at 40 µg/ml (295 sec and 80 sec, respectively). Cytotoxicity was also studied by MTT assay against various cell lines. Against MCF-7, potent activity was observed at 200 µg/ml, while in 3T3 it was observed at 500 µg/ml. Finally, it was observed that these nanoparticles have potent activity against tuberculosis at 1000 µg/ml. The result...
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