Posted by admin on Jun 30, 2020 in |
A simple headspace gas chromatographic (HS-GC) method has been developed and validated for the simultaneous determination of residual solvents like methanol, methyl isobutyl ketone, cyclohexane and toluene in luliconazole by Flame ionization detector (FID). The separation was achieved on 30 m long DB-624 column, 0.53 mm in inner diameter, and 3.0 µm in film thickness. The headspace and chromatographic parameters like flow rate and oven temperature are optimized to enhance sensitivity and chromatographic resolution. Dimethyl sulfoxide is used as diluents, equilibration temperature of 80 °C for 5 min, programmed temperature in the range of 35 °C to 210 °C and nitrogen as the carrier gas was used. The developed headspace gas chromatographic method offers good symmetry and resolution for all the residual solvents. The proposed was found to be suitable for the determination of 4 different residual solvents. Validation results indicated that the method is specific, linear, precise, accurate, rugged, and robust, where recoveries ranged between...
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Posted by admin on Jun 30, 2020 in |
A growing concern of microbial resistance and potential risks associated with existing synthetic preservatives have put industries and researchers under immense pressure to develop newer and safer alternatives based on moieties obtained from natural sources. The purpose of this study was to evaluate the preservative effect of the selected derivatives of naturally occurring phenylpropanoids. Aluminum Hydroxide Gel-USP was employed as a pharmaceutical formulation which has been challenged with Staphylococcus aureus MTCC 737, Escherichia coli MTCC 443, Pseudomonas aeruginosa MTCC 1688, Aspergillus niger MTCC 282 and Candida albicans MTCC 227 as per USP 2004. The selected ester and anilide derivatives demonstrated good antimicrobial potential against all the challenged microorganisms. The preservative efficacy results were comparable to that of standard preservatives, methyl, and propyl-parabens. The benzoin caffeate (CE48) derivative was found to be superior preservative amongst the other derivatives and as well as from both the standards mainly against S. aureus, A. niger and C. albicans. Thus, natural moiety based antimicrobial derivatives has the potential to be chosen as a preservative...
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Posted by admin on Jun 30, 2020 in |
The objective of the present work is to develop and validate a novel stability-indicating RP-UPLC-DAD method for the simultaneous analysis of Metformin and Canagliflozin in bulk and tablet dosage form. The chromatographic separation was accomplished on a Waters UPLC system equipped with autosampler and PDA detector. A volume of 5 µL of sample or standard was injected into the column and the analytes were separated by using the mobile phase containing mixture of 0.1% orthophosphoric acid buffer (pH adjusted to 3.0 with 0.1 N NaOH) and acetonitrile in the ratio 30:70% v/v at a flow rate of 0.25ml/min through C18 BEH (Ethylene Bridged Hybrid) UPLC (100 mm × 2.1 mm 1.7 µm) at 55 °C column temperature and the detector wavelength was set at 260 nm. Peak area and retention time of Metformin and Canagliflozin were found to be 651341 & 332544, 0.994 & 1.508 respectively. Regression analysis shows r value greater than 0.999 for Metformin and Canagliflozin. Percent recovery of Metformin and Canagliflozin was found to be 99.94...
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Posted by admin on Jun 30, 2020 in |
The global prevalence of infectious diseases caused by microorganisms is a major public health concern worldwide. The emergence of antibiotic resistance and related toxicity issues are limiting the use of antibiotics and prompting research for alternative therapy. Plant-derived drugs are gaining popularity due to their efficacy and safety. Calotropis procera and Calotropis gigantea are two common species in the genus Calotropis, well known for their ethnomedicinal values. Present study comparatively evaluated the phytochemical screening and antimicrobial activity of C. procera and C. gigantea. The qualitative phytochemical screening of the ethanolic, methanolic, and aqueous extracts of Calotropis sp. indicated similar phyto-constituents of both the species. Secondary metabolites such as alkaloids, terpenoids, flavonoids, glycosides, cardiac glycosides, coumarin, steroid, phenol, tannin, saponin, and volatile oils were detected in both the plant extracts and ethanolic extracts contained the highest number of the plant metabolites. The plant extracts showed antimicrobial activity against Gram +ve and –ve human pathogenic bacteria, indicating their broad-spectrum activity. Comparatively, C. procera was more effective against the tested bacterial pathogens with lower MIC and...
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Posted by admin on Jun 30, 2020 in |
Plants and plants-based medicines have been used to treat many ailments since the ancient times. Family Orchidaceae to which orchids belongs is the largest family among monocotyledons. The genus Eulophia comprises perennial terrestrial orchids with fleshy tubers, rarely pseudobulbs. One such perennial herb having underground tubers, Eulophia nuda, belonging to family Orchidaceae, has been traditionally used for the treatment of tumours, scrofulous glands of the neck, bronchitis, skin rash and rheumatoid arthritis. In the present study the pharmacognostical and phytochemical evaluation of tubers of E. nuda was performed as it is helpful for the standardization and authentication of medicinal plant. The microscopical study showed the presence of xylem vessels, fibres, starch, raphides and acicular crystals. The phytochemical analysis of the tubers of E. nuda indicated the presence of carbohydrates, alkaloids, flavonoids, steroids, triterpenoids, etc. The results of the HPTLC fingerprinting confirmed the presence of the flavonoids like rutin, quercetin and kaempferol in the tubers of E....
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