Posted by admin on Jun 30, 2020 in |
A simple, precise, and sensitive stability-indicating high-performance thin layer chromatographic (HPTLC) method has been developed and validated for the analysis of Darunavir in bulk and in the tablet dosage form. The separation was performed on pre-coated silica gel 60 GF254 plates using Toluene: Methanol: Triethylamine (8.5:1:0.5 v/v/v) as the mobile phase. The retention factor (Rf) was found to be 0.61 ± 0.89. The detection of a band was carried out at 267 nm. The drug was subjected to different stress conditions like acid, base hydrolysis, oxidation, thermal degradation, and photolysis. The method was successfully validated according to ICH Q2 (R1) guidelines. The linear regression analysis data for the calibration plot showed a good linear relationship with R2 = 0.992 in the range of 500-3000 ng band-1. The method found to be accurate as results of the recovery studies are close to 100%. The developed method can be adopted for routine analysis of Darunavir in bulk and pharmaceutical dosage...
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Posted by admin on Jun 30, 2020 in |
Bombax ceiba is a traditional medicinal plant that is beneficial in the treatment of diuretic, hemostatic, antidysenteric, emetic, antipyretic, astringent, and commonly known silk cotton tree, semal, kapok tree. The aim of this study is to isolate and characterize the biologically active compounds from the ethanolic extract of the leaves. The EtOH extract was subjected to silica gel (60-120 mesh) by glass column chromatography, using gradient petroleum-ether, C6H6, CH3OAc, CH3OH, and purified by preparative TLC (GF254) to yield two pure natural products (taraxerol and β-sitosterol glucoside). The structure was elucidated on the basis of spectroscopic analysis, including 1HNMR, 13CNMR, DEPT-135, COSY, HSQC, and HMBC. The β-sitosterol glucoside shows pharmacological activities such as lowering cholesterol levels, reducing blood pressure, antitumor activity, etc. The glucose moiety is highly effective because it is hydrophilic, which inhibits the entry of cholesterol into the esterification of cholesterol. It is the first time isolated from the leaves of this...
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Posted by admin on Jun 30, 2020 in |
Objective: The present study is designed to investigate vasorelaxation mechanisms of Nanocurcumin in the middle uterine artery (MUA) of both non-pregnant (NP) and pregnant (P) Capra hircus (Ch). Methods: MUA rings were mounted in an automatic organ bath and the contractile response was recorded isometrically by highly sensitive force transducer connected to a power lab data acquisition system. Nanocurcumin (1 pM-100 µM) vasorelaxation response (NCVR) was elicited in PE-precontracted rings either in the absence or presence of different blockers. Results: The maximal vasorelaxation (Rmax) to Nanocurcumin in phenylephrine-precontracted endothelium intact MUA rings of NP (40.36 ± 2.38%) and of P (44.09 ± 3.41%) was reduced to 22.93 ± 0.80% and 25.37 ± 2.2% in endothelium-denuded MUA rings of NP and P Ch. L-NAME, Indomethacin and L-NAME + Indomethacin inhibited NCVR by significantly decreasing the Rmax to 17.65%, 28.42%, 17.95% in NP and to 27.34%, 39.06%, 24.50% in P Ch. The ODQ and carbenoxolone/18β Glycyrrhetinic acid did not inhibit the Rmax of NCVR. Conclusion: Nanocurcumin potentially dilates the MUA of...
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Posted by admin on Jun 30, 2020 in |
The aim of this work is to design and develop a controlled release drug delivery system of the anti-acne agent (dapsone), also used as a leprostatic agent. Dapsone is a strong anti-inflammatory agent that makes it a more powerful treatment in dermatological disorders; its treatment in acne requires long term steady-state concentration in plasma. Poor patient compliance and long term treatment by an oral route leading to dapsone resistance, which stimulates the development of depot preparation. Depot injection consists of a PLGA polymer containing matrix, which gives control release up to 1 month. Microspheres were formed by solvent extraction/evaporation technique. Various parameters, like a selection of solvents, selection of drug: polymer ratio, glycolide: lactide ratio, and evaporation temperature, were important and optimized based on the results. The entrapment efficiency of microspheres was found between 40% to 70%. The initial burst of the drug was controlled and found to be 9.8% within 24 h of release. The inactive ingredients used for formulation development were found to be compatible based on...
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Posted by admin on Jun 30, 2020 in |
The main objective of the current study was to develop a highly sensitive LC-MS/MS (liquid chromatography-tandem mass spectrometry) technique for the quantification of trifluridine (TFD) in human plasma. Chromatography was processed on Phenomenex-RP-C18 (5 µm, 250 mm × 4.6 mm) column comprising acetonitrile, methanol and 5 mM ammonium formate (45:40:15, v/v) as a mobile phase, infused at a flow of 0.8 ml/min. TFD and internal standard ?-thymidine (TMD) were separated from 150 ml plasma utilizing Phenomenex cartridges. Quantification of the [M+H] +ion of analyte was done with MRM (multiple reaction monitoring) modes in LC-MS/MS utilizing electro-spray ionization. The parent and product ions transitions were monitored atm/z 297-181 and m/z2 41–42 for TFD and TMD respectively. The linearity was processed in the concentration range of 5.0–2000.0 ng/ml with regression coefficient (r2) = 0.999. Method intra and inter-batch precision findings were <3.33% and intra and inter-batch accuracy findings at three different concentrations were within 97.34% to 103.74%. Matrix effects were calculated by determining the %CV values for High and Low QC...
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