Posted by admin on Aug 31, 2020 in |
Anti-inflammatory activity and the toxicity of Bohadschia vitiensis water extract were investigated by in-vitro models to evaluate the consumption of the extract by the local community to reduce arthritic-related pain. Specimens of B. vitiensis were collected from Mannar, Sri Lanka, and the water extract (WE) was prepared by removing visceral organs and incubating diced samples in distilled water, followed by freeze-drying. The WE was used to test acute toxicity, based on Danio rerio embryotoxicity assay and to evaluate anti-inflammatory activity based on in-vitro models; egg albumin denaturation, erythrocyte membrane stability, Nitric Oxide (NO) scavenging and Hydrogen Peroxide (HP) scavenging activities. The IC50 was calculated for each assay and compared with a standard reference drug. The qualitative zoo-chemical analysis was carried out while the presence of major nutrients was evaluated. According to the results, WE had a LC50 of 151.59 µg/ml for Danio rerio embryotoxicity assay. The anti-inflammatory activity against egg albumin denaturation (IC50 of 277.51 µg/ml) was reported while a maximum percentage inhibition of 32.09% was reported at 250...
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Posted by admin on Aug 31, 2020 in |
Catalase, guaiacol peroxidase and polyphenol oxidase are antioxidant enzymes that are important in the metabolism of reactive oxygen species and can be induced by environmental stresses including chromium (Cr), heavy metal toxic to living organisms. In-vitro regenerated shoots of carnation (Dianthus caryophyllus L.) were exposed to K2Cr2O7, and the activities of these antioxidant enzymes were analyzed. A significant reduction in the POD activity was observed at 2nd week of culture, followed by a slight increase in the activity in the 4th week of culture. Maximum PPO activity was detected during the 3rd week of culture, which was comparable to the control cultures. A significant reduction in CAT activity was found in the 4th week. The chlorophyll content did not change significantly in the shoot buds developed up to 4th week of...
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Posted by admin on Aug 31, 2020 in |
In the Unani system of medicine, different dosage forms of the drug are used; out of them powder dosage form is known as Safūf, which is an important dosage form and has been used frequently since long. There are many Unani preparations used in the form of Safūf to treat various diseases. Safūf –I Mu’allif (SM) is a polyherbal Unani formulation that is known to be used as Spermatogenic agent and semen thickening agent. The drug is also used to treat sexual disorders like premature ejaculation, nocturnal emission, sexual debility, etc. Physicochemical study and development of standard operating procedures (SOPs) of polyherbal Unani formulations are essential to assess the quality, purity, and efficacy and consistency of drugs. The study formulation consists of following ingredients Tāl Makhānā (Asteracantha longifolia Nees.), Sā’lab Miṣri (Orchis latiflolia L.), Singhārā Khushk (Trapa bispinosa Roxb.), Gond Kīkar (Acacia Arabica Willd.), Māzu Sabz (Quercus infectoria Oliv.), Maṣṭagi Rumi (Pistacia lentiscus L.), Nishāshtah Gandum (Triticum aestivum L), Shaker Sūfed (Saccharum officinarum L.). The main aim of this...
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Posted by admin on Aug 31, 2020 in |
The objective of the present study was to develop and validate a precise and accurate reversed-phase high-performance liquid chromate-graphy for simultaneous estimation of for Fluticasone Propionate (FUP) and Mupirocin (MUP) in combined topical dosage form as per ICH guidelines. Chromatographic separation was achieved using HPLC Shimadzu, Japan, with column syncronis C18 (250 × 4.6 mm, 5 μm). The mobile phase was comprised of 0.01% OPA: Acetonitrile (30:70v/v) (pH: 5) pumped at a rate of 1.0 mL/min. About 20 μL of sample solutions were injected and monitored at 232 nm. Column temperature and sample compartment were maintained at 25° and 5°, respectively. Repeatability, intra, and inter-day precision results were well within the tolerable limits. The linearity was found for range 2.5μg/mL – 7.5μg/mL and 100μg/mL – 300 μg/mL for FUP and MUP respectively. The correlation coefficient of linearity was found to be 0.998 and 0.994 for FUP and MUP, respectively. The limit of detection was found to be 0.3527, and 0.5077and limit of quantification was found to be 1.0690 and...
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Posted by admin on Aug 31, 2020 in |
Superporous hydrogels (SPHs) is originally developed as a novel drug delivery system to retain drugs in the gastric medium by instant swelling on water absorption through open porous structure and maintain their integrity in that harsh environment. Atenolol, an antihypertensive drug with a short half-life, limited bioavailability, unstable nature at basic pH potentiated the need for developing a gastro-retentive system, hence super porous hydrogel of Atenolol had been developed with cellulosic polymers, and adequate strength was imparted by the addition of Ac- Di- Sol. The structural morphology of hydrogel was investigated by SEM, and it was found that plenty of pores of different size ranges, like 1 µm, 2 µm, 10 µm were formed. Compatibility studies proved the integrity of the super porous hydrogel. Gelation time was found to vary with respect to the formulation. The setting time of super porous hydrogel was found to be increased with an increase in the concentration of HPMC K100M. The drug release from super porous hydrogels was sustained for 10 h. In-vitro...
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