Posted by admin on Aug 31, 2024 in |
The objective of this research was to design and validate an alternative accurate method for quantitative determination of Amphotericin B. Amphotericin B deoxycholate life-saving drug belongs to the polyene class approved for the treatment of progressive and potentially fatal fungal infections. Amphotericin B was estimated at 382 nm and the percentage recovery was found to be 100.2 ± 0.34. The method was tested and validated for various parameters according to the ICH guidelines. The repeatability (inter-day) and intermediate precision (intra-day) precision studies revealed that the method is precise and reliable where all the RSD values were less than 2% for the routine analysis of drug. The limit of detection and limit of quantitation were 0.5μg/ml and 1.5μg/ml respectively. Results shows that the developed method is simple, reproducible and successfully for the estimation of Amphotericin B without the interference of common...
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Posted by admin on Jul 31, 2024 in |
Background: Misleading advertisements in the healthcare sector, particularly concerning Ayurveda, Siddha, Unani, and Homeopathy (ASU & H) products, pose significant risks to public health. Despite existing regulations, the prevalence of such advertisements remains a concern, necessitating comprehensive strategies for mitigation. Methods: This cross-sectional analysis examines misleading advertisements reported by the Siddha Regional Research Institute (SRRI), Puducherry, functioning as a Peripheral Pharmacovigilance Centre for Siddha. Data from August 2018 to October 2023 were analyzed, focusing on reported instances, categorization by disease system, prevalent advertising channels, and implications. Results: A total of 69 misleading advertisements were reported by SRRI during the study period. Analysis revealed a notable prevalence of Non-Communicable Diseases (NCD) in reported advertisements, with a significant focus on Diabetes Mellitus. Print media, electronic platforms, and other sources were identified as major channels for advertisement dissemination. Discussion: The findings underscore the urgent need for comprehensive measures to address misleading advertisements on ASU & H drugs. Regional variations in reporting patterns highlight the importance of localized approaches. Regulatory reforms heightened public...
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Posted by admin on Jul 31, 2024 in |
A new simple, specific, accurate and stability-indicating reversed phase `high performance liquid chromatographic (HPLC) method was developed for the determination of Selexipag using a Grace C18 (250mm x 4.6ID, Particle size: 5 micron) a mobile phase consisting of Buffer (pH-7.4): Methanol 60:40, at a flow rate of 1.0 mL/min and ultraviolet detection at 294 nm. The retention times of Selexipag was found to be 4.296 min. Linearity was established for Selexipag in the range of 20-100 μg/mL with correlation coefficients >0.999. The percentage recovery of Selexipag was found to be in the range of 100.99-101.06%. Stress testing was carried out to demonstrate specificity of the method. The developed method could separate the potential degradation products from the Selexipag. This proposed method was suitable for analysis of the content of Selexipag in Pharmaceutical dosage form. The method is validated as per ICH...
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Posted by admin on Jul 31, 2024 in |
Cortisol is one of the vital glucocorticoids secreted by the adrenal glands and is also referred to as the “stress hormone” as the synthesis and release of cortisol by the adrenal glands is directly proportional to the stress levels in an individual. It is a catabolic hormone that stimulates gluconeogenesis and has an indirect role in liver and muscle glycogenolysis. It facilitates lipid, protein and carbohydrate metabolism. Over the years medicinal grasses have been in the highlight for their potent medicinal value. Hence, Wheatgrass was chosen for study. Wheatgrass is derived from the common wheat plant. It is the grass of the common wheat plant. It belongs to the Poaceae family. Wheatgrass is the freshly sprouted first leaves of the wheat plant (Triticum aestivum). The Wheatgrass extract was obtained from methanol. Phytochemical screening was conducted which showed the presence of carbohydrates, phenols, flavonoids, terpenoids, tannins, alkaloids, glucosides, proteins, steroids, saponins and fixed oils. Wheatgrass was taken and cell viability assay was done using MTT dye. This assay indicated that methanolic extract...
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Posted by admin on Jul 31, 2024 in |
This study aimed regarding application of comprehensive stability-indicating analytical method for estimation of Escitalopram impurities in multiple brands of pharmaceutical dosage forms using reverse phase high-performance liquid chromatography. The comprehensive stability-indicating analytical method applied utilized an isocratic mode with a C18 column. The mobile phase comprised a phosphate buffer and acetonitrile (75:25v/v) at a flow rate of 1.2ml/min. A sample injection volume of 5μL was employed, and eluted analytes were monitored at 240nm. The utilized method was validated following ICH guidelines and found to be simple, specific, highly sensitive, precise, robust, and linear for Escitalopram in the range of 0.16 to 2.4μg/ml. Similarly, for Escitalopram impurities A, B, D, H, L & C, the observed linearity range was 0.25 to7.5μg/ml. Accuracy range was confirmed for Escitalopram from 0.16 to 2.3μg/ml, Escitalopram impurities A, B, H from 0.3 to 8μg/ml, and Escitalopram impurities C, D, and L from 0.5 to 8μg/ml. The solution demonstrated stability for up to 48 Hrs. Additionally, the applied method was proven to be stability-indicating by...
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