Posted by admin on Sep 30, 2020 in |
Quality is the most important requirement in the manufacturing process for every pharmaceutical and healthcare industry. All the drugs must be manufactured to the highest quality level. Quality cannot be guaranteed just by end-product testing, but we have to control each critical step carefully during the manufacturing process. This qualification and computer system validation plays an important role in controlling each critical step of application in order to maintain the quality of the final product. Computer system validation involves a series of activities that are taking place during the life cycle of processes. It also involves careful planning of various stages in the qualification and validation of application/software used in the manufacturing process, and all the work should be carried out in a structured way according to standardized working procedures. The objective of this work is to overview the qualification and computer system validation of instrument/equipment used in the pharmaceutical...
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Posted by admin on Sep 30, 2020 in |
The objective of the present study is to develop a colon targeted drug delivery system by using sodium alginate as a carrier for Mesalamine. Matrix tablets containing various excipients and sodium alginate were prepared by wet granulation technique using different binder systems. The prepared tablets were evaluated for hardness, weight variation, drug uniformity, friability, and in-vitro drug release study. The surface of the device of the best formulation was coated with Eudragit S100 to ensure that the device was more pH-dependent and trigger the drug release only at higher pH. The matrix tablet containing sodium alginate as a carrier and hydroxypropyl methylcellulose as a binder was found to be suitable for targeting mesalamine for local action in the colon as compared to other matrix tablets containing different binders. Matrix tablets containing sodium alginate released 97-99% of mesalamine in the simulated colonic fluid. The stability study for prepared tablets at 40 ºC/75% relative humidity for three months showed no significant change in the in-vitro drug release pattern. The results of...
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Posted by admin on Sep 30, 2020 in |
Herbal medicines play an important role in cancer prevention. Mangroves have been used worldwide due to the presence of various bioactive metabolites in it. The specific medicinal properties of Rhizophora mucronata have been an interest in preventing breast cancer. Mangroves Rhizophora mucronata belongs to family Rhizophoraceae. The leaf extract reveals various roles in folk remedies to treat various diseases. This analytical study aims to find out various phytochemical and components present in the leaf extract by the GC-MS technique. The methanol leaf extract revealed the presence of phytochemicals like saponins, flavonoids, alkaloids, quinines, glycosides, cardiac glycosides, terpenoids, phenols, coumarins, and steroids. The GC-MS analysis provided nine different compounds like Phenol, 2, 4-bis (1,1-dimethyl ethyl)- (28.2%), Flavone- (66.9%), 4H-1-benz0pyran-4-one,7-hydroxy-2phenyl (39.8%), Chrysin (100%), 4H-1 Benzopyram-4-one, 7-hydroxy-2-phenyl-(100%), 5,8 Octadecadienoicacid, methyl ester-(55.8%), Phytol- (31.8%), Coumarine, 3-(2,4-dinitrophenyl)-(31.3%), Elaidic acid, isopropyl ester-(9.2%). Further purified compounds are used in the pharmacological industry against breast cancer and reduce the side effects and pains during the treatment of...
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Posted by admin on Sep 30, 2020 in |
Adaptation of marine actinobacteria to extreme climatic conditions such as high salinity, high pressure and high temperature has modified their physiological conditions to survive and elaborate novel bioactive metabolites. Among the different actinobacterial strains isolated from coastal regions of Guntur District, Andhra Pradesh, India, one potent strain with broad-spectrum antagonistic activity was found and identified as Streptomyces rectiverticillatus VJMS-8. Attempts were made to optimize the cultural parameters for an enhanced yield of antimicrobial metabolite production. Production of bioactive metabolites by the strain was high in tryptone yeast extract dextrose broth as compared to the other media tested. The strain utilized galactose and tryptone as good carbon and nitrogen sources for the elaboration of bioactive metabolites. The optimum temperature and pH for bioactive metabolite production by the strain were recorded as 35 °C and 7.0, respectively. NaCl @ 5% supported high pro-duction of bioactive metabolites. The secondary metabolites produced by the strain grown under optimal conditions exhibited high antagonistic activity against a variety of gram-positive and gram-negative bacteria as well...
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Posted by admin on Sep 30, 2020 in |
The objective of present research work is design and in-vivo evaluation of ibrutinib self-nanoemulsifying drug delivery system (SNEDDS) for the enhancement of oral bioavailability. Box-Behnken design was used for the study and the results analysed using response surface methodology. Ibrutinib SNEDDS were prepared with various oils, surfactants and co-surfactants and tested particle size, PDI, zeta potential, refractive index, drug release and TEM studies. The optimized ibrutinib SNEDDS has the composition of capryol 90, cremophore EL and transcutol HP. Based on the particle size and entrapment efficiency and in-vitro dissolution studies; F2 is identified as optimized formulation with 85% drug released, whereas less than 1% was released from pure drug at end time of 30 min. In-vivo bioavailability data reported in male wistar rats indicate higher concentration of drug in plasma implying enhanced systemic absorption of ibrutinib from SNEDDS formulation. The Cmax and AUC0-inf values of optimized SNEDDS was considerably higher (p<0.05) than pure drug formulation. The oral bioavailability of optimized SNEDDS increased around 12 folds when compared with pure...
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