Posted by admin on May 31, 2020 in |
Present study was conducted to identify and characterize the phytoconstituents of methanolic extract of leaves of Tephrosia falciformis (Pers.) Ramaswami of family Fabaceae. The methanolic extract of leaves was subjected to qualitative screening for primary and secondary metabolites as per standard methods; further GC-MS analysis was carried out for the identification of secondary metabolites. The preliminary analysis of leaf extract confirmed the presence of primary as well as secondary phytoconstituents such as phenolic compounds, alkaloids, flavonoids, terpenoids, phytosterols, etc. in methanolic extract. GC-MS analysis of extract revealed the presence of 53 phytochemicals. The presence of various compounds with different chemical groups like quinolene, piperdinone (alkaloids), Neophytadiene (sesqueterpenens), phytol (diterpene alcohol), sitosterol, stigmasterol (sterols) and many fatty acids including their derivatives in considerable amount gives the good prospect for the use of this plant in pharmaceutical preparations to cure health-related...
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Posted by admin on May 31, 2020 in |
Cyanobacteria are a gram-negative group of microorganisms with unique features and found in diverse conditions of the environment. These tiny bio-factories are rich in pigment molecules like chlorophylls, carotenoids and phycobiliproteins. Studies on physiological and biochemical characteristics of different cyanobacterial strains will not only help to determine their biotechnological potential but also determine and interpret the appropriate growth conditions of these tiny microorganisms in their natural habitat. This paper focuses on the growth, chlorophyll, carotenoid and phycobilin production in two freshwaters (Anabaena cylindrica and Anabaena PCC7120), one halotolerant (Anabaena KP737864) and two marine cyanobacterial strains (Synechococcus BDUSM-13 and Nostoc BDUMM-70). Significant differences were observed among the strains with regard to their pigments. Maximum chlorophyll and phycobiliprotein content were observed on the 14th day of cultivation in all strains. The filamentous cyanobacteria Anabaena cylindrica recorded maximum production of phycocyanin, marine Synechococcus BDUSM-13 produced maximum phycoerythrin (17.33 µg/ml). Anabaena KP737864 was reported to acquire more amount of allophycocyanin (12.66 µg/ml) of...
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Posted by admin on May 31, 2020 in |
Ulcerative colitis is a subtype of Inflammatory Bowel Disease. The leaves of Syzygium cumini belonging to the family Myrtaceae were selected to evaluate its efficacy in ameliorating the acetic acid-induced Ulcerative colitis. Male albino Wistar rats were randomly divided into five groups. Group 1 was given the vehicle tween 80, group II received 2 ml of 4% acetic acid solution on 8th day intrarectally, group III was given 2 ml of 4% acetic acid solution once intrarectally on 8th day and 2mg/kg of prednisolone orally for 3 days starting from the day of acetic acid treatment. Group IV and V received 7 days pretreatment with 250 mg/kg and 500 mg/kg of ethanolic leaf extract of Syzygium cumini (SCEL), respectively, and 2 ml of 4% acetic acid solution once intrarectally on 8th day. Drug treatment was continued till 10th day. The rats were sacrificed on the 11th day, and the hematological, macroscopical and biochemical parameters were assessed. Pre-treatment with the extract improved the hematological parameters, body weight, and the stool...
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Posted by admin on May 31, 2020 in |
In view of the biological prominence of the benzoxazole derivatives as well as, isatin derivatives, it was planned to synthesize some novel N-(Benzoxazol-2-yl)-2-(2-oxoindolin-3-ylidine) hydrazine carbothioamides (VI) as such reports were not available in the literature and were screened for antibacterial, antifungal and anti-mycobacterial activity. Fourteen new compounds were synthesized by condensing different Isatins (V) with N-(Benzoxazol-2-yl) hydrazine carbothioamide (IV). All the prepared compounds were screened for antibacterial, antifungal, and antimycobacterial activities on various microbial strains. The results revealed that all the synthesized compounds were exhibiting antimicrobial properties. Compound VIc, VIe, VIg, VIi, and VIl were declared to possess potent antimicrobial properties in the given bacterial and fungal...
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Posted by admin on Apr 30, 2020 in |
A simple, selective, precise, and stability-indicating RP-HPLC method has been developed for the determination of Curcumin in bulk forms and solid dosage formulations. The HPLC separation was achieved on Waters Spherisorb ODS2 column (4.6mm × 250mm, 5µm particle size) using a mobile phase of methanol-water (77:33 v/v, pH 3) at a flow rate of 1ml/min and spectrophotometric detection at 419 nm. The method was validated for specificity, linearity, accuracy, precision, solution stability, robustness, system suitability, and ruggedness. The detector response for the Curcumin was linear over the selected concentration range from 1 to 10 µg/ml, with a correlation coefficient of 0.9993. The accuracy was between 99.27& 101.97%. The precision (R.S.D.) among the six sample preparations was 1.27%. The LOD and LOQ are 0.092 and 0.278 µg/ml, respectively. The recovery of curcumin was about 100.12%. The drug was subjected to acid and alkali hydrolysis, oxidation, wet heat treatment, and photodegradation. The drug undergoes degradation under former conditions, which indicates that the drug is susceptible to acid and base hydrolysis, wet...
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