Posted by admin on Jun 30, 2020 in |
Parkinsonia aculeata (family – Caesalpiniaceae) is a large spinous shrub found in warmer regions of the world. It is a well known medicinal plant that has significant and promising biological activities such as antioxidant, anti-inflammatory, antidiabetic, antispermatogenic, etc. The phytochemical investigations of the leaves, flowers, and stem have revealed the presence of various glycosides, flavonoids, reducing sugars, alkaloids, sterols, tannins, volatile oils, and saponins. The various biological activities and potent phytoconstituents of the plant indicate its therapeutic potential efficacy. The present study shows the detailed investigation of the methanolic stem bark extract of the title plant. From the methanolic stem bark extract of the title plant 1-cyclohexyltridec-1-ene (new compound A), 6-hydroxypentacosylpentanoate (B), α-amyrin acetate (C), α-amyrin (D) and β-sitosterol (E) were isolated with the help of column chromatographic technique and characterized on the basis of different spectral studies i.e., 1H NMR, 13C NMR, IR, and Mass...
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Posted by admin on Jun 30, 2020 in |
Carvedilol is a non-selective β blocker with α1 blocking activity used in the treatment of congestive heart failure, high blood pressure, and left ventricular dysfunction. Following administration by the oral route, it undergoes extensive first-pass metabolism resulting in low bioavailability of 25-35%. The aim of the present study was to incorporate carvedilol in bilayered chitosan containing mucoadhesive buccal patches to ensure unidirectional drug release. Buccal administration circumvents hepatic first-pass metabolism, as the drug directly enters into the systemic circulation through the buccal mucosa, thereby increasing systemic bioavailability. The patches were prepared using solvent casting method. Chitosan was used as a mucoadhesive polymer as well as the base matrix for the drug. To improve film properties of the patches, PVP was incorporated in all the formulations. An impermeable backing layer of ethylcellulose was used to ensure the unidirectional release of the drug. The compatibility of carvedilol and polymers was confirmed by DSC and FTIR. Bilayered patches were evaluated for various physicochemical parameters like appearance, weight and thickness, folding endurance, tensile...
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Posted by admin on Jun 30, 2020 in |
A simple headspace gas chromatographic (HS-GC) method has been developed and validated for the simultaneous determination of residual solvents like methanol, methyl isobutyl ketone, cyclohexane and toluene in luliconazole by Flame ionization detector (FID). The separation was achieved on 30 m long DB-624 column, 0.53 mm in inner diameter, and 3.0 µm in film thickness. The headspace and chromatographic parameters like flow rate and oven temperature are optimized to enhance sensitivity and chromatographic resolution. Dimethyl sulfoxide is used as diluents, equilibration temperature of 80 °C for 5 min, programmed temperature in the range of 35 °C to 210 °C and nitrogen as the carrier gas was used. The developed headspace gas chromatographic method offers good symmetry and resolution for all the residual solvents. The proposed was found to be suitable for the determination of 4 different residual solvents. Validation results indicated that the method is specific, linear, precise, accurate, rugged, and robust, where recoveries ranged between...
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Posted by admin on Jun 30, 2020 in |
A growing concern of microbial resistance and potential risks associated with existing synthetic preservatives have put industries and researchers under immense pressure to develop newer and safer alternatives based on moieties obtained from natural sources. The purpose of this study was to evaluate the preservative effect of the selected derivatives of naturally occurring phenylpropanoids. Aluminum Hydroxide Gel-USP was employed as a pharmaceutical formulation which has been challenged with Staphylococcus aureus MTCC 737, Escherichia coli MTCC 443, Pseudomonas aeruginosa MTCC 1688, Aspergillus niger MTCC 282 and Candida albicans MTCC 227 as per USP 2004. The selected ester and anilide derivatives demonstrated good antimicrobial potential against all the challenged microorganisms. The preservative efficacy results were comparable to that of standard preservatives, methyl, and propyl-parabens. The benzoin caffeate (CE48) derivative was found to be superior preservative amongst the other derivatives and as well as from both the standards mainly against S. aureus, A. niger and C. albicans. Thus, natural moiety based antimicrobial derivatives has the potential to be chosen as a preservative...
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Posted by admin on Jun 30, 2020 in |
The objective of the present work is to develop and validate a novel stability-indicating RP-UPLC-DAD method for the simultaneous analysis of Metformin and Canagliflozin in bulk and tablet dosage form. The chromatographic separation was accomplished on a Waters UPLC system equipped with autosampler and PDA detector. A volume of 5 µL of sample or standard was injected into the column and the analytes were separated by using the mobile phase containing mixture of 0.1% orthophosphoric acid buffer (pH adjusted to 3.0 with 0.1 N NaOH) and acetonitrile in the ratio 30:70% v/v at a flow rate of 0.25ml/min through C18 BEH (Ethylene Bridged Hybrid) UPLC (100 mm × 2.1 mm 1.7 µm) at 55 °C column temperature and the detector wavelength was set at 260 nm. Peak area and retention time of Metformin and Canagliflozin were found to be 651341 & 332544, 0.994 & 1.508 respectively. Regression analysis shows r value greater than 0.999 for Metformin and Canagliflozin. Percent recovery of Metformin and Canagliflozin was found to be 99.94...
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