Posted by admin on Apr 30, 2020 in |
Alzheimer’s disease (AD) is a major type of dementia. AD is associated with memory impairment and cognitive deficit. It is characterized by low levels of acetylcholine in the brain of AD patients. The inhibition of acetylcholinesterase (AChE) has been one of the most used strategies for the management of mild to moderate AD. In the present study, n-hexane, ethyl acetate, and hydroalcoholic leaf extracts of two medicinal plants Phyllanthus emblica (Euphorbiaceae) and Tamarindus indica (Fabaceae) were screened for anticholinesterase activity and compared with standard acetyl-cholinesterase inhibitor (galantamine) by Ellman’s spectrophotometric method. The results obtained from the three extracts of P. emblica and T. indica against AChE enzyme was evaluated for percentage inhibition and IC50 values. Among the extracts of P. emblica, ethyl acetate extract (96.62 ± 0.165) at a concentration of 200 µg/ml was found to be potent. Ethyl acetate extract of T. indica showed very potent inhibition (97.55 ± 0.087) at the concentration of 200 µg/ml and found to be equally potent with that of galantamine (98.63 ±...
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Posted by admin on Apr 30, 2020 in |
The RP-HPLC stability-indicating assay method has been developed and validated for the estimation of the amlodipine and chlorthalidone in bulk and combined dosage form. The method was optimized by using the mobile phase as a mixture of 0.1% formic acid: methanol: acetonitrile in the ratio of (50:5:45 v/v) at pH 3 was adjusted with orthophosphoric acid. The method was carried out on the Octadecylsilane C18 column (5 µm, 25 cm × 4.6 mm) using a flow rate of 1.0 ml per min. The method was scanned at λmax 266 nm for both the drugs using a PDA detector. The retention time was found to be at 6.32 min and 5.32 min for AML and CHL respectively. The calibration curve determined at respective retention time is found to be 2.5-7.5 μg/ml and 06-18 μg/ml with a regression coefficient of 0.9990 and 0.9940 for AML and CHL respectively. The developed and validated method is reliable, simple, precise and accurate and easy to apply in the...
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Posted by admin on Apr 30, 2020 in |
Cognitive functions are mainly categorized into memory, attention, creativity, and intelligence. Cognitive enhancement may be defined as the amplification or extension of core capacities of the mind. Phenylethylamine is an organic compound which is also known as β-phenylethylamine (β-PEA) and is the name of a class of chemicals which shows psychoactive and stimulant effects. It is a trace amine and a natural monoamine alkaloid. PEA plays an important role in weight loss, treating depression, ADHD and Alzheimer’s disease, etc. The main objective of this study is to synthesize derivatives of ethylamine and phenyl-ethylamine, which may inhibit MAO receptors and improve cognitive behavior. All synthesized derivatives were studied for their biological evaluation on the brain, and they showed the good neurological activity and found effective to reverse the memory deficits induced by scopolamine. While, there is no significant effect was seen in the locomotor effect, which represent that these agents are effective to control the motor...
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Posted by admin on Apr 30, 2020 in |
The validated protein precipitation method was applied for the estimation of Baloxavir marboxil in human plasma with Oseltamivir as an internal standard (ISTD) by using HPLC-ESI-MS/MS. The chromatographic separation was achieved with 0.1% formic acid in combination with methanol (25:75 v/v) using the C18 column Ascentis Express (50 mm × 4.6 mm, 2.7 µm). The total analysis time was 3 min and the flow rate was set to 0.6 ml/min. The mass transitions of Baloxavir marboxil and Oseltamivir obtained were m/z 572.6®250.3 and 313.2®269.1. The standard curve shows a correlation coefficient (r2) greater than 0.9983 with a range of 5.00-10000.00 pg/ml using the linear regression...
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Posted by admin on Apr 30, 2020 in |
A simple, rapid, accurate, robust, and specific HPLC method was developed for the assay of metformin hydrochloride and teneligliptin hydrobromide from the oral tablet formulations. Stability-indicating reverse-phase chromatographic method was developed on a RP C18 column (250 mm × 4.6 mm, 5µm) using a mixture of 20 mM ammonium acetate of pH 5.5 and methanol in the ratio 50:50 v/v as mobile phase in an isocratic mode of elution at a flow rate of 1.0 ml/min at 35 ºC with a load of 20 µl. The detection was carried out at 255 nm. The method was validated with respect to linearity, robustness, precision, accuracy, specificity & stability as per ICH guidelines. The method produced excellent separation with good linear correlation coefficients (≥ 0.999) for both the components. The proposed method could be successfully applied for the assay of metformin hydrochloride and teneligliptin hydrobromide from tablet...
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