Posted by admin on Jul 31, 2024 in |
Objectives: Grewia hirsuta Vahl. is a shrub explored for medicinal applications. It has anti ageing, boosting immunity, prevents loss and grey hair etc. Ethnopharmacological data suggests that the plant is extensively used as a nerve tonic and to enhance the cognitive properties. The basis of these medicinal uses seems to be antioxidant and anti cholinesterase activity. In order to establish the scientific basis between medicinal uses and its mechanism of action, antioxidant and anti cholinesterase activity investigations were carried out. Methods: Evaluation of free radical scavenging and anti cholinesterase activity was carried out using DPPH (1, 1- diphenyl-2-picryl hydrazyl), hydroxyl radical scavenging and ferric reducing method using ascorbic acid as a standard. Cholinesterase inhibitory potentials were measured by Ellman’s method against Rivastigmine. Results: The inhibitory concentration of hydro ethanolic extract of Grewia hirsuta (HEEGH) (IC50 27.25) in DPPH. In reducing power assay (IC50 65.32) and Hydrogen peroxide scavenging activity (IC50 98.28). The inhibitor concentration of acetylcholinesterase by standard (Rivastigmine) was (IC50 14.40μg/ml), and the extract was (IC50 133.4μg/ml), whereas,...
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Posted by admin on Jul 31, 2024 in |
Objectives: Valvular Heart Disease (VHD) is an important cause of cardiovascular morbidity and mortality worldwide. The aim of this study was to establish the frequency of VHD on the basis of echocardiographic parameters, assess etiological factors, quantify the clinical spectrum and evaluate the medical management of VHD. Methods: This observational cross-sectional study was conducted for a period of 5 months from May to September 2021 at a tertiary care hospital in Tamil Nadu. A total of 101 patients, both in-patients and out-patients visiting the cardiology department were included for the study. Results: Among the 101 participants, the mean age was 55.43 ± 14.91 with 55.4% of them being males. The most common etiology was found to be degenerative (75.2%). The most commonly encountered valve lesions included mitral regurgitation (67.3%) followed by aortic regurgitation (36.6%), tricuspid regurgitation (33.7%) and mitral stenosis (21.8%). The prevalence of VHD and VHD of degenerative etiology increased with age >40. The occurrence of VHD symptoms was also higher with age >40 (P<0.001). Conclusion: The major...
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Posted by admin on Jul 31, 2024 in |
Fresh Aloe vera plant leaves were brought from botanical garden and sample was identified and brought to the laboratory in the Department of Zoology, Patkar-Varde College, Goregaon (W), Mumbai. 50 grams of leaves were then grounded with 50ml of distilled water in sterilized pestle and mortar. The yield will be calculated based on weight of the extract compared to the weight of the pulp of the leaves. Forty-eight (48) Sprague- dawley rats (average weight 150 – 250 g) of either sex were used for the experiment. The ETH and PAS drug and Aloe vera juice were given to respective groups daily for 28 days. At the end of the study animals were anesthetized and were sacrificed by cervical decapitation. Blood was collected via cardiac puncture and studied for WBC: White blood cell count, LYM: Lymphocytes, MID: Indicates the combined value of the other types of white blood cells not classified as lymphocytes or granulocytes, NEUT: Neutrophils, RBC: Red blood cell count, HGB: Hemoglobin, HCT: Hematocrit and PLT: The platelet counts...
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Posted by admin on Jul 31, 2024 in |
A Liquid Chromatography-Mass Spectrometry (LC-MS/MS) method was used for quantification of potential genotoxic impurities (PGI) in the Darunavir propylene glycolate drug substance (API). Chromatographic separation was achieved using a column UPLC BEH C18, 1.7 μm (100×2.1mm), with a Mobile-phase-A was ammonium bi carbonate and Mobile-phase-B was acetonitrile in gradient elution mode at a flow rate of 0.3 ml/min and injection volume is 2.0 µL. Quantification of impurities was carried out using triple quadrupole mass detection with electrospray ionization in multiple reaction monitoring mode. The method was fully validated with good linearity over the concentration range of 0.23 to 1.69 μg/g of the Darunavir propylene glycolate test concentration for all Genotoxic impurities. The correlation coefficient obtained in each case was 0.999. The recoveries were found satisfactory over the range between 98.1 to 101.4% for all selected impurities. A novel selective, highly sensitive and hyphenated analytical method using LC-MS/MS coupled with negative electrospray ionization has been developed for quantification of genotoxic impurities like Nitro benzene sulfonic acid and Methyl-4-amino benzene sulfonate...
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Posted by admin on Jul 31, 2024 in |
Background: To reduce the run and retention times of amlodipine and chlorthalidone in pharmaceutical dosage forms, a simple, affordable, time-efficient, stability-indicating, and accurate reverse-phase liquid chromatographic technique was developed. Successful chromatographic separation was achieved using Inertsil ODS-3V (250 x 4.6 mm, 5 μm particle size). A gradient consisting of water (pH 3 adjusted with formic acid) and acetonitrile was used as the mobile phase. A 20 μl injection volume was utilized, and a flow rate of 1 ml per minute was observed. The wavelength of detection was 365 nm. Method validation followed ICH norms. The medication was also found to be stable under all stress scenarios. The observed retention times for amlodipine were 1.8 minutes and chlorthalidone was 4.5 minutes. The linearity range was observed in a concentration of 50 – 150%. The percentage recovery of Chlorthalidone was 99.30% and for Amlodipine was 99.61%. The suggested technique was successfully tested and used for the estimation of amlodipine and chlorthalidone in pharmaceutical dose...
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