Posted by admin on Dec 31, 2024 in |
Coagulase negative Staphylococcus, always regarded as nonpathogenic common commensal of skin and mucous membrane, are emerging as ubiquitous endemic nosocomial and oppurtunistic pathogens in recent times. They usually are considered as culture contaminants, thus left untreated which lead to their increased pathogenic potential. Methicillin resistance in Coagulase negative Staphylococcus is of significant concern as these strains are also multi drug resistant. The purpose of this study was to estimate the frequency of Methicillin resistance in CoNS in clinical samples and determine their biofilm producing ability and in-vitro antimicrobial susceptibility. A total of 2240 various clinical samples like urine, pus, blood, swabs received during one year period from January to December 2023 were processed in clinical laboratory, Department of Microbiology, Andhra Medical College, King George Hospital, Visakhapatnam. CoNS were isolated and identified by biochemical reactions and methicillin resistant strains identified by cefoxitin and oxacillin disc diffusion method and tested for biofilm production by Congo red agar and tissue culture plate method. Antimicrobial susceptibility done by conventional Kirby Bauer disk diffusion...
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Posted by admin on Dec 31, 2024 in |
The current study reports to validate a stability indicating RP-HPLC method for estimation of mitapivat in bulk, pharmaceutical formulations and FD characterization by using MS method. The chromatographic separation was accomplished on Waters X-Bridge phenyl column (150× 4.6mm, 3.5µm). With the mobile phase consisting of acetonitrile and 0.1% OPA (80ː20v/v) at a flow rate of 1ml/min. The eluents were monitored by UV detector at 234nm. The developed liquid chromatographic method was validated with respect to accuracy, precision, linearity, robustness, range, limit of detection (LOD), limit of quantification (LOQ). The drug was subjected to various stress conditions such as acid, alkali, peroxide, reduction, photolytic, hydrolysis and thermal degradation. Among all the stress condition 5degradation products were obtained they were DP-1, Dp-2, Dp-3, Dp-4, Dp-5. They were subjected to mass characterization. The obtained structures were subjected to in-silico studies using Swiss ADME, pk CSM web server, protox2. The SWISS ADME web server and pk CSM web server were used for the prediction of pharmacokinetic properties and toxicity of the drug and...
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Posted by admin on Dec 31, 2024 in |
The Purpose of research was to study process validation for Dapagliflozin 10 mg, Sitagliptin 100 mg and Metformin Hydrochloride 500 mg (Extended Release) Bilayered Tablets. The critical process parameters were identified with the help of process capability and evaluated by challenging its lower & upper release specifications. Three initial process validation batches (I, II, III) of the same size, method, equipment & validation criteria were taken. The critical parameters involved in sifting, dry mixing, preparation, preparation of granulating agent, wet mixing, wet milling, drying, sizing, lubrication, compression stage, coating & finished stage were identified and evaluated as per validation master plan. The process indicated that this process validation provides high degree of assurance that manufacturing process produces a product meeting its predetermined specification and quality attributes. Process validation is the validation of each and every step of the processes which involves series of activities carried out in order to have the assurance of the products manufactured. Each and every step should be scientifically planned, conducted and documented appropriately and...
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Posted by admin on Dec 31, 2024 in |
Objective: To understand the magnitude of vaccine hesitancy, its proximal determinants, and recommend specific interventions to address it in Mewat district (also known as Nuh), Haryana. Design: A mixed-method study design was adopted which involved: Household survey (479 households) and key informant interviews with healthcare providers (10 service providers). Setting: Four blocks namely, Nuh, Ferozepur-Jhirka, Punahana, and Taoru of Mewat district, Haryana. Results: In Mewat, age-appropriate vaccination was observed in 48.8% of the children, whereas 31.6% were partially vaccinated and 19.6% were unvaccinated. Fear of side effects of vaccination (35.0%) was one of the main reasons for partial and no immunization. About 22.1% of households in Mewat were either hesitant or refused to vaccinate their children. Out of all four blocks, Punahana block had 33.3%, the highest proportion of vaccine hesitant or refusal households. A strong patriarchal society with low women empowerment was observed in the community while interacting with beneficiaries and front-line-workers. Further, there existed a misconception that vaccines cause infertility, which leads to gender disparity in immunization...
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Posted by admin on Dec 31, 2024 in |
Alcohol-related liver diseases and their associated damage pose significant health concerns globally. Among potential remedies, esculetin, a saturated dicarboxylic acid known for its anti-inflammatory and antioxidant attributes, stands out. This study aims to explore the hepatoprotective effects of esculetin in rat models afflicted by liver damage induced by ethanol+ lipopolysaccharide (LPS). To emulate alcoholic liver damage, adult Wistar rats were subjected to 10 days of ethanol (5 mL/kg b.w) and a single dose of LPS (5 mL/kg b.w) orally on the 11th day. Esculetin was administered orally to rats for the same 11 days duration, at doses of 50 mg/kg b.w, with a one-hour interval before inducer administration. The results revealed a remarkable reduction in the elevated levels of serum enzymes AST, ALT, ALP, and GGT following esculetin treatment, effectively restoring them to normal levels. Moreover, esculetin exhibited a substantial decrease in liver lipid peroxidation levels compared to the ethanol and ethanol+LPS group, indicating its potent management of severe oxidative stress. The addition of esculetin to the ethanol and...
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