Posted by admin on Mar 31, 2020 in |
Obesity ailments, commonly known as “New World Syndrome,” has drawn the attention of the scientific community to explore novel natural product-based therapeutics as effective alternatives. In this study, different solvent extracts of Moringa oleifera (M. oleifera; Drumstick tree) leaves were prepared, and their phytochemical analysis was done. The therapeutic effect of M. oleifera leaf extracts on key enzymes related to obesity and against oxidative stress was studied through standard assays (DPPH, ABTS, NO, FRAP). Among different solvent extracts, the M. oleifera leaf hydroalcoholic extract (MOHE) contained a high amount of polyphenols (135.45 ± 3.435 μg/mL) and flavonoids (129.52 ± 3.102 μg/mL). The IC50 values of MOHE for α-glucosidase, α-amylase, and pancreatic lipase were 373.6 μg/mL, 339.8 μg/mL and 437.1 μg/mL respectively which were much lower than rest of the M. oleifera extracts. The GC-MS analysis of MOHE showed the presence of many glycosides, oils and polyphenolic compounds, as mentioned in the results section. In conclusion, our data suggest that M. oleifera leaves are rich in several bioactive phytochemicals that qualify it as an...
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Posted by admin on Mar 31, 2020 in |
Introduction: The present study designed to investigate Human parvovirus B19 (HPV-B19) as an etiological agent of rash–fever ill patients confirmed negative for measles and rubella infection which is responsible for the same symptoms in North Indian population. Materials and Methods: Total 319, negative serum samples selected after measles/rubella IgM antibodies detection. The patients between the age of (4 to 52 years) with fever and rash-like illness were divided into four groups: 4-14, 15-25, 26-36 and >37 years old. The serological analysis was done by Indirect IgM ELISA and Real-time PCR used for molecular identification of HPV-B19 in this study. Results: The prevalence of HPV-B19 was found to be 29.4% by both methods in Measles and Rubella negative serum samples. We found a 29.4% positive result by HPV-B19 IgM ELISA and 7.5% positive by Real-time PCR against HPV-B19 infection. The results have been statistically significant (p= 0.0001*). The highest prevalence (38.5%) was found between 15-25 years of age group. Conclusion: The study was designed to find the frequency of HPV-B19...
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Posted by admin on Mar 31, 2020 in |
Mating disruption by an artificial release of synthetic female pheromone components like Tetradecyl acetate (A), Ethyl (Z)-9-hexadecenoate (B) and a mixture of these two at different concentrations is a feasible method to control Xanthopimpla predator. Male Xanthopimpla predator was evaluated for responses to these synthetic pheromone components in a laboratory wind tunnel and in Terminalia arjuna field. Septa loaded with different concentrations (0.2, 0.4, 0.6, 0.8 and 1.0 mg) of Tetradecyl acetate (A), Ethyl (Z)-9-hexadecenoate (B), A and B blend in 1:1 ratio (C) has disrupted the male Xanthopimpla predator orientation in the wind tunnel and blend with 1.0 mg had shown more attraction towards Xanthopimpla predator. Mating disruption efficacy in tasar field was evaluated in first, second and third crops with 16 traps in each crop. In all crops, 5 traps baited with pheromone loads of 0.2, 0.4, 0.6, 0.8 and 1.0 mg of Tetradecyl acetate, 5 traps with Ethyl (Z)-9-hexadecenoate and 5traps with a blend of both in 1:1 ratio. One blank control trap without any type...
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Posted by admin on Mar 31, 2020 in |
A forced stability-indicating method for simultaneous estimation of glecaprevir and pibrentasvir by Ultra Performance Liquid Chromatography (UPLC) was developed and validated. The separation was done on column BEH C18 50 × 2.1mm 1.7m which was maintained at 30 °C, eluted with isocratic mobile phase and eluents were detected at 260nm. Mobile phase containing Buffer 0.01NKH2PO4: Acetonitrile taken in the ratio 50:50 was pumped through column at a flow rate of 0.3 ml/min. The retention time of glecaprevir and pibrentasvir was found to be 0.392 min and 0.607 min respectively. Validation with respect to specificity, system suitability, precision, accuracy, linearity, and robustness was performed as per ICH guidelines. % RSD of the glecaprevir and pibrentasvir were and found to be 0.5 and 0.7 respectively. % Recovery was obtained as 100.40 and 99.90% for glecaprevir and pibrentasvir respectively. LOD, LOQ values obtained from regression equations of glecaprevir and pibrentasvir were 0.79, 2.38 and 0.51, 1.55 respectively. Regression equation of glecaprevir is y = 2140x + 994.9 and of pibrentasvir was y...
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Posted by admin on Mar 31, 2020 in |
A new simple and sensitive liquid chromatography-electrospray ionization-tandem-mass spectrometric (LC-ESI-MS/MS) technique was developed and validated for the estimation of modafinil in human plasma and the method was applied to study pharmacokinetics in healthy rabbits. Chromatography was attained on Phenomenex- C18 (50mm × 4mm) 5µ column and acetonitrile, methanol and 0.1% formic acid (25:60:15 v/v) mixture as the movable phase at 0.7 ml/min flowrate. Modafinil and modafinil-D5 internal standards were detected at m/z 274.2/229.0, m/z 279.1/234.0 respectively. Modafinil and modafinil-D5 (internal standard) were separated with liquid-liquid extraction. The technique was linear over the 2.0-600.0 ng/ml concentration range. This technique established with intrabatch and inter batch precision within 1.54-7.18% and 1.82-6.25%. This technique established with intrabatch and inter batch accuracy within 98.56-102.80% and 97.62-102.76 %.The drug was shown mean Tmax of 3.833; average Cmax, AUC0͢ t and AUC0͢ α, for test formulation is 677.667; 6306 and 6471 respectively in the pharmacokinetic study on healthy...
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