Posted by admin on Jan 31, 2020 in |
The validated analytical method was applied for estimation of vorapaxar in aqueous and human plasma with vorapaxar-D5 as an internal standard by using UPLC-ESI-MS/MS. The chromatographic separation was achieved with 5mM Ammonium Formate Buffer (pH: 4.0): Methanol: Acetontrile, (40:30:30, % v/v) using the Pursuit XRs-100Å, C18, 4.6 × 50 mm, 10 µm. The total analysis time was 3.5 min and flow rate was set to 0.2 ml/min. The mass transitions of vorapaxar and vorapaxar-D5 obtained were m/z 591.4®447.2 and 498.6®447.2. The standard curve shows correlation coefficient (r2) greater than 0.999 with a range of 0.01-200.0 ng/ml using the linear regression model. The developed method was applied successfully for studying the pharmacokinetics of vorapaxar in rabbits....
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Posted by admin on Jan 31, 2020 in |
In the current scenario, high concentrations of acrylamide (ACR), a water-soluble vinyl monomer, which is used industrially and also found in thermally processed carbohydrate-rich foods, poses serious health risks to humans. Oxidative stress is an important mediator of central and peripheral neurotoxicity, which is one of the most recognized toxicological effects of high concentration acrylamide exposure in humans and experimental animals. The present study was designed to investigate the protective effect of Morus nigra fruit extract against acrylamide induced neurotoxicity modeled in Danio rerio (Zebrafish). Mulberry fruit is rich in mostly polyphenolic compounds such as anthocyanins and flavonoids. Forty zebrafishes were randomized into three experimental groups, (I) normal control group, (II) 0.75mM ACR treated group and (III) post ACR fruit extract-treated group. Upon completion of 72 h of ACR-exposure, a few exposed fishes were randomly selected for treatment with the fruit extract. In the ACR-treated group, a significant elevation in lipid peroxidation with concomitant depletion in the activities of catalase, glutathione reductase and acetylcholinesterase were observed in the zebrafish...
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Posted by admin on Jan 31, 2020 in |
AIM: To monitor the adverse drug reactions due to various antidepressants prescribed in patients coming to psychiatry OPD with depression at a tertiary care hospital in northern India. Methods: This was a prospective observational longitudinal study conducted for a period of 12 months in a tertiary care outpatient facility. Patients who fulfilled the inclusion and exclusion criteria and those who gave informed consent were recruited. The antidepressant prescribed to the patient was noted and the patient was followed for a period of 6 months for complaints of any adverse effects. Results: A total of 96 patients were monitored for adverse drug reactions. ADRs were only recorded in the SSRIs group of patients of which only escitalopram and sertraline were prescribed to patients. 20.8% of the patients developed adverse events of which the most common were nausea, followed by a headache. Other observed adverse events that were recorded were anxiety, dry mouth, vomiting, somnolence, fatigue and sexual dysfunction. Conclusions: Prevention of adverse drug reactions occurring due to antidepressants can be...
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Posted by admin on Jan 31, 2020 in |
Neuropathic pain is commonly comorbid with mood disorders, such as depression and anxiety. A state of depression may suggest biochemical imbalance that can increase the oxidative stress into the brain. The present study aimed to investigate the effect of aqueous extract of the red algae Sphaerococcus coronopifolius on depression-induced behavioral alterations and oxidative stress in female Wistar rats. Sciatic nerve injury was employed to induce depression in rats. Antidepressant-like effect of S. coronopifolius was investigated using two models: the forced swimming test (FST) and the open field test (OP). Three groups of rats were considered: The first group served as a control. The second group was subjected to sciatic nerve ligation (untreated group). The third group was subjected to sciatic nerve ligation treated daily by intraperitoneal injection of 25 mg algae extract kg−1 body weight of rat during two consecutive weeks(treated group). At the end of the experiment, rats were sacrificed and brain tissues were isolated to determine the activities of catalase (CAT) and glutathione-S-transferase (GST) as well as...
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Posted by admin on Jan 31, 2020 in |
The present study was aimed to assess the hepatoprotective activity of ethanolic extract of Coleus vettiveroides on experimentally induced hepatotoxic models. Phytochemical screening of EECV was carried out and the results showed the presence of carbohydrates, alkaloids, tannin, flavonoids, phenolics, proteins and aminoacid, steroids and triterpenoids. HPTLC screening of EECV was carried out along with different marker compounds to ensure the presence of phytoconstituents such as Quercetin, Rutin, and Gallic acid. Antioxidant activities of the herbal extract were determined using both DPPH and ABTS scavenging method. MTT assay is most widely used to determine the cytotoxic potential of medicinal agents and other toxic materials. It was observed that for different concentrations of EECV, the cell viability was greater than 90%. In-vitro hepatoprotective activity of EECV was evaluated on Hep G2 cells, the human hepatoma cell line. In Hep G2 treatment with EECV (12.5, 25, 50, 100 and 200 μg/ml) the cell viability was above 90% indicating that EECV showed no cytotoxicity up to 200 μg/ml. Evaluation of biochemical parameters...
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