Posted by admin on Jul 31, 2024 in |
Background: Daucus carota L contains a variety of carotenoids and well known for their antioxidant properties, in particular β-carotene. The antioxidant capability of polyphenols present in Camellia sinensis has been reported to be both chemo-protective and healing. The present work is about to evaluate the Hepatoprotective activity of Daucus carota L and Camellia sinensis extract combination against azathioprine-induced liver injury in rats as a model and determine the hematological findings, biochemical parameters and histophathological changes in liver section of rats. Methods: To evaluate the hepatoprotective activity of combination animals were divided into 6 groups of six rats in each group. Group I a normal control group which received normal saline on the daily basis by orally. Group II a disease group treated with drug model azathioprine 50mg/kg (w/w) i.p route to induce disease. Group III a standard drug group and the rats are treated with drug silymarin 50mg/kg (w/w) p.o Group IV test drug 1 (carrot extract) 25ml/kg body weight by p.o route. Group V test drug 2 (Green...
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Posted by admin on Jul 31, 2024 in |
Development of UV spectrophotometric method for the estimation of Hydrochlorothiazide was done by Q-Absorbance ratio method and area under curve method and stability indicating studies using methanol as solvent. In the present research, we have made an attempt to develop a simple, specific, accurate, precise and reproducible method for the estimation of hydrochlorothiazide by UV spectrophotometric method, the method includes area under curve method (Method I) and Q- absorbance Ratio method (Method II). The wavelength is 272 nm λmax of drug was selected for Method I, and for Q- absorbance Ratio method (Method II) 258 nm an iso-absorptive wavelength and 272 nm were selected for estimation of Hydro-chlorothiazide and the drug follow Beer’s law over the concentration range of 1-6 μg/ml. The % recovery of the drug was found to be nearly 100 % representing the accuracy of the proposed methods. LOD and LOQ values of hydrochlorothiazide were Found to be 0.136, 0.411, 0.181, 0.556, 0.134, 0.404 at 272 nm, 258 nm and 242 nm respectively, validation of the proposed...
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Posted by admin on Jul 31, 2024 in |
Traditionally, Merremia vitifolia meets multipurpose medicinal uses in tribal areas. Therefore, the study worked on hypoglycemic and hepatoprotective effects on animal models to meet the research gap on this plant. Methanolic stem extract at 200 mg/kg and 400mg/kg were administrated orally to determine the effects on blood glucose and hepatic enzymes. The highest dose showed a significant (p < 0.01) reduction in blood glucose. Maximum glucose level reduction (52.1%) was observed at 400 mg/kg extract with 3.5±0.6 mmol/L after 3 h, while reference glibenclamide (10mg/kg) reduced 25.2% with 4.4±0.8 mmol/L. The plasma levels of alkaline phosphatase, alanine aminotransferase, and aspartate aminotransferase of CCl4 intoxicated mice were 108.8, 43.4, and 268.8 IU/L at 400 mg/kg, where the values for reference silymarin (100 mg/kg) were 103.4, 54.3, and 237.7 IU/L, respectively. The reduction of alkaline phosphate and alanine aminotransferase levels were moderately (p<0.01) and minimally (p<0.05) significant at concentration 400 mg/kg, respectively. Therefore, the study explored the plant’s potential hypoglycemic and hepatoprotective effects, where the hypoglycemic effect was strongly...
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Posted by admin on Jul 31, 2024 in |
Secondary metabolites found in various plants play an important role in curing various diseases and are used as important raw materials for the production of traditional and modern medicine. One of these plants, Paspalum conjugatum, a member of the Poaceae family, has been used for different purposes since prehistoric times. In this research work, we extracted the powdered leaves of P. conjugatum by the sequential extraction method using four different solvents (n-hexane, chloroform, ethanol, and water). About eight phytochemical analyses were carried out, out of which four phytochemicals (alkaloid, tannin, flavonoid, and glycoside) were significantly identified in both n-hexane and ethanol extracts. The proximate analysis was carried out for the leaves powder. The moisture content, total ash, acid-insoluble ash, and water-insoluble ash were found to be 3.56, 9.76, 3.22, and 27.08%, respectively. Reducing power assay, total antioxidant activity, total phenolic content, total flavonoids, and reduction of ferric ions were also investigated as a part of the antioxidant test. All extracts showed a significant presence of antioxidants. The ethanol extract showed...
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Posted by admin on Jul 31, 2024 in |
Vernal keratoconjunctivitis (VKC) is a chronic inflammatory condition affecting the cornea and conjunctiva of young children. Health-related quality of life (HRQoL) is impaired in children with VKC. Mast cell stabilizers are often offered as the first-line therapy for VKC. In this study, the effects of two types of mast stabilizers on HRQoL were compared.This was a prospective, open-label, randomized, and comparative clinical study. Patients were randomized to receive one of two mast cell stabilizers-bepotastine or olopatadine. The HRQoL was assessed using the Quality of Life in Children with Vernal Keratoconjunctivitis (QUICK) questionnaire at baseline and after 3 weeks. The change in the QUCIK score was recorded to see the impact of treatment on HRQoL. The mean age of the cohort was 9.6 ± 2 years. Most patients presented in the months of April and May. A statistically significant (p <0.1) change of over 60% in QUICK scores was observed in both groups, but there was no statistically significant difference (p 0.07) in the QUCIK questionnaire score at 3 weeks...
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