Posted by admin on Dec 31, 2019 in |
Green synthesis is one of the best routes for the silver nanoparticles (AgNPs). The present study revealed that the aqueous leaf extracts of Aeschynomene aspera, which contains alkaloids, flavonoids, phenols, terpenoids, anthocyanidins, indoles, glycosides, saponins and tannins, is found to be responsible for bioreduction during the synthesis of silver nanoparticles (SNPs). The colour change from light yellow to dark brown within 20 to 60 min indicates the formation of nanoparticles. The UV-Visible spectrum of the aqueous medium containing silver nanoparticles showed a Surface Plasmon Resonance peak at 450 nm. FT-IR analysis reveals that the phenols and amides are mainly responsible for reduction. XRD analysis clearly showed the characteristic Bragg peaks of (111), (200), (220), (311) facets of the face-entered cubic structure of silver nanoparticles and confirmed that these nanoparticles are crystalline in nature.HR-TEM analysis revealed that the green synthesized SNPs were spherical in shape with an average size of 10 nm. The antibacterial effect of synthesized AgNPs on different bacterial strains with the zone of inhibition 14.1 mm on...
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Posted by admin on Dec 31, 2019 in |
Organophosphorus (OP) nerve agents blocks acetylcholinesterase and do not allow the degradation of acetylcholine. Post-treatment of organophosphate poisoning involves the treatment with oxime reactivators as an antidote, which will dephosphorylate the phosphate bond between enzyme and nerve agent. Due to CNS permeability failure of these oximes, the introduction of low molecular non-oximes has become a challenging role for reactivation of inhibited acetylcholinesterase. In-silico high-throughput structure-based virtual screening and docking by using LOPAC database targeting CNS for inhibited acetylcholinesterase reactivation. For the identification of an active site of AChE enzyme and high throughput virtual screening, Inventus™ software was being used. ADME analysis for the selection of leads which can follow up Lipinski rule of 5 and CNS parameters for permeation. Further docking studies of selected lead candidates was done using Schrodinger software. By taking various CNS parameters into consideration hits were selected. For further refinement docking was performed, and the best lead obtained with glide XP docking score -11.656 Kcal/mol. The best lead candidates passed CNS permeation parameters range and...
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Posted by admin on Dec 31, 2019 in |
Hardwickia binata belongs to family Fabaceae / Caesalpiniaceae and commonly known as ‘Anjan’. The present study includes phytochemical screening, FTIR and GC-MS analysis. Shade dried powdered leaves, seed and husk of H. binata extracted in petroleum ether, chloroform, ethyl acetate, acetone, methanol, ethanol, distilled water using Soxhlet apparatus and used for phytochemical analysis. Crude powder of plant used for FTIR analysis and ethanolic extracts was analyzed using GC-MS. The phytochemical screening of H. binata revealed the presence of carbohydrates, proteins, amino acids, steroids, glycosides, flavonoids, lipids, tannins, phenolic compounds, quinones, mucilage, volatile oils, fats, and fixed oils whereas alkaloids, emodins and resins were not detected in any extracts of leaves, seed, and husk. The majorities of compounds are present in acetone, methanol, ethanol and distilled water and glycosides, lipids and mucilage are present in all extracts. FTIR analysis showed the presence of alcohol, phenols, amines, amides, carboxylic acids, aromatics, alkenes, alkanes, aliphatic amines, esters, ethers, alkynes, alkyl halides in leaves, seed and husk; saturated aliphatic found in only seed...
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Posted by admin on Dec 31, 2019 in |
Introduction: Cutaneous adverse drug reactions (CADRs) associated with significant morbidity and mortality are probably the most frequent of all manifestations of drug sensitivity. Material and Methods: It was a prospective observational study where newly diagnosed patients with ADRs reporting to OPD of Dermatology, K.G.M.U, Lucknow and satisfying inclusion criteria were enrolled. The various study tools used were the suspected ADR reporting form (CDSCO), Naranjo’s causality scale, Modified Hartwig and Siegel severity scale and Dermatology Life Quality Index. Results: In a total of 124 patients recorded with CADRs, males (60.5%) were found more affected than females (39.5%). The most common age group found was 21-30 yrs (36.3%) followed by 31-40 yrs (25.8%) with a mean age ± SD 35.88 ± 13.87 range (18-78) years. The most common clinical pattern observed was Fixed Drug Eruption (FDE) (49.2%) followed by maculopapular rash (MPR) (36.3%). The incidence of Severe CADRs (SCADR) was 8.06%. Antimicrobial (50.8%) followed by unknown (17.7%), combinations (14.5%) and anti-epileptics (8.9%) were the most common drug groups suspected. On the...
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Posted by admin on Dec 31, 2019 in |
A selective and rapid LC-MS/MS spectrophotometric method has been developed and validated for the simultaneous determination of three ACE-inhibitors used in anti-hypertensive therapy, namely enalapril maleate, perindopril, and ramipril in human plasma using high-performance liquid chromatography-tandem mass spectrophotometry (LC-MS/MS) with electrospray ionization (ESI). Separation of analytes and internal standard; atorvastatin was performed on X-terra C8 (3.5 µm, 4.6 × 50 mm) column with a run time of 2 min. The mobile phase consisted of methanol: 20 mM ammonium formate, pH 6.3 ± 0.05 with formic acid (80:20, v/v). Analytes were extracted from human plasma using a simple protein precipitation technique with methanol, allowing fast analysis. The method was validated in terms of accuracy, precision, selectivity, recovery and stability as per FDA and EMA guidelines. The method showed linearity over the concentration range 4- 400 ng/mL, 2 – 200 ng/mL and 0.5 – 50 ng/mL for enalapril, perindopril and ramipril, respectively, applying weighted (1/X2) linear regression. The method is simple, fast, precise, accurate and suitable for its application for bioequivalence...
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