Posted by admin on Dec 31, 2019 in |
Electron microscopic studies revealed variations in different cell organelles in steroidogenic cells of normal, regressing and pregnant caprine corpora lutea. Corpus luteum was comprised of two types of steroidogenic cells viz. small theca luteal cells and large granulosa luteal cells. In granulosa luteal cells, rough endoplasmic reticulum was most abundant in corpus luteum of pregnancy. The number of mitochondria, Golgi complex and secretory granules increased from normal to pregnancy category and decreased in regressing category. In theca luteal cells, smooth endoplasmic reticulum, number of mitochondria and Golgi complex were more in pregnancy. During regression, degenerating luteal cells with shrunken cytoplasm, dispersed nuclear membrane, apoptotic granules, increased number of lipid droplets, lysosomes, swollen mitochondria, irregular plasma membrane, disintegrating and vacuolated rough endoplasmic reticulum were observed. The degenerating luteal cells of regressing corpus luteum revealed the patches of chromatin material along the periphery of the uneven nuclear membrane and also within the nucleoplasm. These variations shall be discussed in relation to their physiological...
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Posted by admin on Dec 31, 2019 in |
Drug-drug interactions (DDIs) are known for their potential to cause adverse clinical outcomes, rate of morbidity and mortality. An integrated approach to cognitive pharmaceutical care (CPS) would decrease the incidence of DDIs in hospitalized patients. This prospective interventional study was carried out among the inpatients admitted to a tertiary care multispecialty hospital in Chennai. A total of 749 drugs were accumulated from 100 in-patients. A perpetual continuous quality improvement (CQI) program was conducted to edify and train the healthcare staff on a weekly basis with counter-current data collection on DDIs. The results 645 DDIs in which 153 (23.72%) were major, 348 (53.95%) were moderate and 144 (22.32%) were minor. The CPS recommendations like dose adjustment, time of drug administration, alternate drug choice etc. were incorporated into the DDIs. The post CQI results depicted a gradual decrease in the prevalence of DDIs for a period of 3 months. This study revealed the pertinence of CPS in the management of potential DDIs. The need for structured, as well as integrated approaches...
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Posted by admin on Dec 31, 2019 in |
The aim of this research was to perform phytochemical screening and evaluation of the pharmacological activities of Buchanania lanzan Spreng. leaves. Preliminary phytochemical screening revealed the presence of phytoconstituents like steroids, tannins, saponins, carbohydrates, alkaloids, and flavonoids. Three leaf extracts were prepared by using solvents e.g. chloroform, ethyl acetate, and ethanol. The antibacterial activity was evaluated by using the paper disc method against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. The chloroform extract was found to be more effective against Pseudomonas aeruginosa with a zone of inhibition 20 mm as compared to the standard anti-biotic ampicillin with a zone of inhibition 20 mm. Antifungal activities were evaluated by well diffusion assay against Candida albicans, Aspergillus flavus, Aspergillus niger. The chloroform extract was found be more effective against Aspergillus flavus and Candida albicans with a zone of inhibition 22 mm and 20 mm respectively as compared to the standard antifungal ketoconazole with a zone of inhibition 22 mm. Antifungal activity against Aspergillus niger with zone of inhibition 22 mm was...
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Posted by admin on Dec 31, 2019 in |
The research herbal formulation prepared by mixing dried stem barks of Azadirachta indica A. Juss. and Saraca asoca Roxb. in equal amounts is standardized through pharmacognostic and phytochemical studies for assessing its efficacy in the treatment of leucorrhoea/vaginitis/ excessive white discharge. Pharmacognostical analysis revealed total ash value of 9.43% having 1.89% acid insoluble ash, 4.62 pH and 7.70% moisture content and presence of nitrogen and halogens. Flavonoids, tannins, carbohydrates, glycosides, saponins and fixed oils were found present in the hydro-alcoholic extracts. The results also showed a high concentration of flavonoids compounds (43.37 µg quercetin equivalent/mg) and high phenolic content (101.22 µg gallic acid equivalent/mg). UV-Visible spectroscopy scanning showed a prominent peak at 277 nm in the hydro-alcoholic extract. HPTLC analysis at 254 nm, 277 nm, and 330 nm indicated the presence of tannic acid, gallic acid, quercetin and catechol. Similarly, HPLC analysis at 277 nm suggested the presence of gallic acid, tannic acid and catechol. FTIR analysis exhibited the presence of N-H stretching, C-H stretching, N-H bending, N-O asymmetric...
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Posted by admin on Dec 31, 2019 in |
Analytical method for simultaneous estimation of Ertugliflozin (ERT) and Metformin hydrochloride (MET) was developed and validated by high-performance liquid chromatography (HPLC) as per ICH guidelines. The drugs were injected into the inertsil C18 (250 × 4.6 mm) maintained at room temp and wavelength 220 nm. The mobile phase consists of buffer (potassium dihydrogen pH 4.0) and methanol (65:35 v/v). The flow rate is maintained at 1.0 mL/min. The calibration curve was linear and regression coefficient (R2) value was found to be 0.999 and concentration ranging from 1.5-4.5 µg/mL and 100-300 µg/mL for Ertugliflozin and Metformin hydrochloride respectively. The LOD and LOQ of the method were found 1.04 μg/mL, 9.61 µg/mL and 0.0007 µg/mL, 0.006 µg/mL for Ertugliflozin and Metformin HCl. The developed method was found to be simple, precise, specific, linear and accurate as validated as per USP and International Conference on Harmonization (ICH)...
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