Posted by admin on Dec 31, 2019 in |
Arnebia benthamii (synonym – Microtomia benthamii) commonly known as Ratanjot or Laljari found in western Himalayan region and used as wound healer roots by local vaids and tribes. The roots of Arnebia benthamii contain a red dye that is used in various health disorders like fungal infection, inflammation, fever and coloring/flavoring agent in Indian curries. To estimate wound healing potential of root extract of Arnebia benthamii, percentage wound contraction, wound area, epithelization time, tensile strength and wound index were measured in 13 days of wound healing study. The results showed highly significant wound healing in excision as well as incision wound healing models (P<0.01). The Arnebia benthamii extract-treated wounds showed about 41% higher wound contraction rate and 44% increased tensile strength in comparison with negative control animals. The epithelization time decreased by 43% and wound index value decreased by 89% in comparison to negative control animals which indicated that methanolic root extract of Arnebia benthamii is very helpful in faster and high-quality wound healing (P <...
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Posted by admin on Nov 30, 2019 in |
The research work focused on the optimization of Terbinafine HCl (TBH) pluronic lecithin organogels (PLOs) for increasing the penetration and efficacy of the drug. The factors affecting the PLOs such as viscosity and percentage drug release, were screened and optimized using the Box-Behnken design. The optimum formulation based on desirability (0.995) exhibited 3139.92 Cp for viscosity and 93.4561% for drug release. The optimized PLO was evaluated for drug content, pH, in-vitro drug release and ex-vivo studies. The percentage cumulative release of TBH permeated from optimized PLOs and marketed cream (MC) at the end of 12 h after the application was found to be 60.29 ± 0.69% and 19.35 ± 2.19% respectively. The comparatively higher permeability TBH in optimized PLOs could be due to increased solubility and diffusion coefficient of the drug. In-vitro microbial assay, studies showed that the PLO reduced the fungal burden of Candida albicans as compared to M.C (0.34 ± 0.08 mm) in a shorter duration of time with a larger zone of inhibition (0.58 ± 0.12...
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Posted by admin on Nov 30, 2019 in |
This study aimed to investigate the preparation and characterization of polymeric nanoparticles containing extract of Andrographis paniculata using Eudragit S 100 polymer. The methanolic extract of aerial parts of Andrographis paniculata Nees (Acanthaceae) was prepared and standardized for andrographolide content using the High-Performance Liquid chromatography technique. Three formulations containing 50, 100 and 150 mg of extract were prepared by solvent displacement method. Parameters including drug: polymer ratio, stabilizer, solvent, and stirring speed were optimized. Characterization of the formulations was carried out using particle size analysis, PDI, DSC, X-RD, SEM techniques, entrapment efficiency and in-vitro drug release studies. The particle size of the nanoformulations was found to be within the range of 300-400 nm, with entrapment efficiency more than 75% and drug release more than 55% after 8 h. Aerial parts extract of Andrographis paniculata was embedded and dispersed in Eudragit S 100 polymer as smooth spherical nanoparticles which may prove to be a novel drug delivery...
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Posted by admin on Nov 30, 2019 in |
A simple, selective, linear, precise and accurate RP-HPLC method was developed and validated for quantification of Darunavir in bulk and its tablet dosage form. The separation was carried out on Zorbax Eclipse XDB C18 (150 × 4.6 mm; 5 µm) column at 35 °C temperature using ammonium acetate buffer as mobile phase-A, acetonitrile (100%) as mobile phase-B. The flow rate was 1.0 ml/min and effluent was detected at 265 nm. The retention time of Darunavir propylene glycolate was 31.34 min. The percentage recovery was within the range between 95.46% and 100.17% for Darunavir propylene glycolate. The linear ranges were found to be 0.086-3.084 µg/ml (r2 = 0.9997) for Darunavir propylene glycolate. The percentage relative standard deviation for accuracy and precision was found to be less than 5.0%. Hence, the method could be successfully applied for routine analysis of Darunavir in pharmaceutical...
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Posted by admin on Nov 30, 2019 in |
The blood-brain barrier is the most challenging physiological obstacle for drug moieties for reaching the central nervous system. Meropenem is a drug used against meningitis, however crossing BBB is also a challenging realm of problem for the same. Therefore attempt has been undertaken to strategically design Meropenem loaded cholesterol-soya lecithin nanoliposomes (MCSLN). MCSLN was prepared by nano-emulsification technique with tween 80 as its surface ligand. Molecular docking studies revealed that tween 80 interacts with apolipoprotein E through π-π interactions and hydrogen bonding, which might be its mechanism for transmembrane permeation across BBB. After characterization, in-vitro release study was carried out to evaluate cross-walking efficiency of liposomal drug delivery system through the simulated blood-brain barrier. Entrapment efficiency estimation showed that about 80% of the drug has been successfully entrapped within the liposome. Dynamic light scattering study exhibited bimodal distribution of particle with size ranges 70-75 nm and 300-350 nm. Zeta potential reveals good stability of the formulation. FTIR studies reveal that Meropenem has been successfully entrapped within the formulation. On...
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