Posted by admin on Jul 31, 2024 in |
Introduction: Aceclofenac (ACE) and Curcumin (CUR) are anti-inflammatory drugs utilized for osteoarthritis and rheumatoid arthritis. ACE, As NSAID inhibits prostaglandins, while CUR, a natural polyphenol, exhibits antioxidant and anticancer properties. Combining them may offer synergistic benefits for inflammatory diseases. Objective: To develop a UV spectrophotometric method for simultaneous determination of ACE and CUR from a developed dosage form, focusing on simplicity, Accuracy and cost-effectiveness. The method employs absorbance correction to calculate drug concentrations based on their absorbance difference at two wavelengths. Method: Using a solvent of phosphate buffer pH 5.5: ethanol (1:1), standard solutions of ACE (5-30 µg/mL) and CUR (1-7 µg/mL) were prepared. UV spectrophotometer measured absorbance at 276 nm and 430 nm for both standard and sample solutions. Absorbance correction equations were used for concentration determination. Result: Method validation followed ICH guidelines, demonstrating good linearity (correlation coefficients: 0.999 for ACE, 0.998 for CUR), accuracy (recovery values: 92.72% for ACE, For cur) and precision (RSD < 2% for intra-day and inter-day variations). Specificity was confirmed, and method robustness...
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Posted by admin on Jul 31, 2024 in |
Shilajit, a herbo-mineral blackish brown resin, extracted from humic rocks, is attributed to several therapeutic claims in Ayurveda like antiaging, health-promoter, immune- booster, urogenital problems and bio-enhancer. The lack of strong parameters for its quality control and standardization; and the knowledge gap to explain its mechanism of action are the important factors behind its restricted use. Here, for the first time, we have used a High-Resolution Advance Mass Spectrophotometer (HRAMS) to visualize the metabolites of shilajit and further identified novel metabolites for its characterization. Seven brands of shilajit, available in the market (5 resins and 2 capsules), were purchased and used to validate our findings in terms of molecular weight, abundance, retention time and structural characteristics. We found 14 novel metabolites and six of them were acidic, eight were non-acidic. Among them, only 4 metabolites were common in all seven samples, which were (a) NP-020214, (b) 3-tert- Butyladipic acid, (c) Dodecanedioic acid and (d) Azelaic acid. However when only resins were compared, then additional nine common metabolites were identified...
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Posted by admin on Jul 31, 2024 in |
Introduction: Self-medication is becoming more and more common in our daily lives but it’s an unhealthy and risky habit. It assumes that second year MBBS students holds special significance as they start to deepen their knowledge about diseases and medications, which forms a critical foundation for their medical education and future practice. This is the only study conducted in the northeastern region of India till date. The objective of the study is to assess the knowledge, attitudes, and practices regarding self-medication among second-year MBBS students at Silchar Medical College and Hospital. A cross-sectional survey was conducted using a pre-formed questionnaire including both open and close ended questions among the second year MBBS students over a period of one month after taking written inform consent from the participants. Data was collected by using google form and result expressed as counts and percentage. Result: Out of 120 participants, 72(60%) were males and 48(40%) were females. Out of them total 116 students took self-medication and among them 90 students provided reasons in...
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Posted by admin on Jul 31, 2024 in |
The aim of this study was to investigate the antibacterial activity of Psidium guajava leaf extract in periodontal film for inserting in periodontal pockets. The study was performed by formulating bioerodible periodontal film containing Psidium guajava leaf extract by simple solvent casting method using two different polymers namely natural chitosan and semi synthetic HPMC. The formulated periodontal films were then cross linked with 2 % v/v glutaraldehyde for 3 hours for extending the release of drug. The periodontal films were evaluated for various physico-chemical parameters. The cumulative percentage drug release was found to be higher for periodontal film prepared with chitosan polymer. The results from the in-vitro release studies of Psidium guajava leaf extract from different batches of periodontal film have shown that the release followed zero order release kinetics for the batch F4. The results of release data for batch F4 fits well in Higuchi model, suggesting diffusion mechanism of drug release. The research findings have concluded that both chitosan and HPMC could be used for preparing periodontal...
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Posted by admin on Jul 31, 2024 in |
Vitis vinifera (Grape) encounters several difficulties during their shelf life, including weight loss, browning and softening. Packaging is one of the effective solutions to preserve fruits from postharvest loss. Tridax procumbens (T. procumbens) leaves are rich in bioactive compounds and possess good antioxidant and antimicrobial activity. This study evaluates the efficiency of T. procumbens leaf extract film as a coating on V. vinifera (V. vinifera) to increase postharvest storage life at 20-25℃. Different concentrations of T. procumbens leaf extract incorporating PVA film were successfully developed, coated on V. vinifera and stored for 21 days. Throughout the storage period, at 7 day intervals, rate of weight loss and changes in the content of titratable acidity, ascorbic acid, total phenolic, reducing sugar and DPPH scavenging activity were assessed. During storage, the T. procumbens leaf extract film-coated V. vinifera effectively inhibited the weight loss rate and slowed down the loss of titratable acidity, ascorbic acid content, total phenolic content and DPPH activity in contrast to that of non-coated V. vinifera. Therefore, the...
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