Posted by admin on Nov 30, 2024 in |
Black rice is a rice species, Oryza sativa Linne (Gramineae), which is sticky, high in nutrients, and primarily grown in Asia. In recent years, the health benefits of black rice varieties have been reported due to the presence of a bioactive compound, anthocyanin, which has highly potent activity with high macronutrients. The present study aimed to evaluate and quantify the metabolites, focusing on describing the structure of Cyanidin-3-O-glucoside (CY3G) as a biomarker of anthocyanin present in the extract of black rice. Four different black rice varieties black rice extracts of Poireiton (BREP), Kokngangbi (BREK), Amubi (BREA), and Sempak (BRES) were collected, and phytochemical analyses performed using different standard methods. Compared to other black rice varieties, Amubi, BREA has the highest phenolic (63.23±0.76 mg/g GAE), flavonoid (28.09±0.68 mg/g QE), and anthocyanin (17.69±0.45 mg C/g DEW) content. It also exhibited the highest potent antioxidant activity, with IC50 values of 29.1 ± 1.11 mg/ml in the ABTS assay and 98.83 ± 0.61 mg/ml in the DPPH assay. The FTIR spectroscopy analysis result...
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Posted by admin on Nov 30, 2024 in |
This study focuses on optimizing the tissue culture of Aegle marmelos, a medicinal plant with diverse pharmacological properties. The research fine-tuned sterilization methods, identifying a 2% sodium hypochlorite solution as the most effective. Callus induction experiments revealed the optimal hormonal combination for Aegle marmelos as MS medium + Auxin-2,4-D (0.5 mg/l) + Cytokinin-BAP (1.5 mg/l). Suspension cell cultures were then developed as a foundation for further experiments. The study also explored permeabilizing agents (Triton X-100 and Tween-80) in Aegle marmelos cell cultures, finding that Tween-80 enhances the extraction of secondary metabolites more effectively than Triton X-100. High-Performance Liquid Chromatography (HPLC) analysis validated these outcomes. In conclusion, this research significantly contributes to optimizing plant tissue culture protocols for Aegle marmelos, advancing scientific understanding, and offering practical insights for improving the reproducibility and efficiency of Aegle marmelos cell cultures. These findings hold promise for potential publication, marking notable advancements in plant tissue culture and permeabilization...
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Posted by admin on Nov 30, 2024 in |
Background: Integrity is the cornerstone of pharmacovigilance. Underreporting is the main issue being faced by Pharmacovigilance programme. It is our moral duty as law-abiding citizens and medical professionals to report adverse drug reactions (ADRs) in order to protect patients’ health. Aim and Objective: To study about the knowledge, attitude, practice of Pharmacovigilance among second professional MBBS students of SMCH. Materials and Methods: This is a questionnaire based cross sectional study. After gaining informed consent, 128 students were given the pre-tested, pre-validated questionnaire (knowledge 1–12, attitude 13–18, and practice 19–23) via Google form. Thirty minutes later the response was gathered. Microsoft Excel version 21 was used to statistically analyse the collected data. Result: Response rate was 100%. Of the participants, 97.7%, 81.3%, 85.9%, 87.5%, and 85.9% correctly answered questions about the definition of pharmacovigilance, the distinction between adverse drug reactions and adverse events, how to report adverse drug reactions, and the goals and techniques of pharmacovigilance. With regard to mindset, all students were aware of the importance and advantages of...
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Posted by admin on Nov 30, 2024 in |
Flavonoids are a class of Polyphenolic compounds found abundantly in plants. This study employs computational methods to predict the binding affinities and interaction patterns of selected flavonoids with the adenosine A2A receptor, Monoamine oxidase B (MAO-B enzyme), and Catechol-O-Methyltransferase (COMT enzymes). The docking simulations were performed using software packages such as iGEMDOCK which utilizes molecular mechanics algorithms to simulate the docking process. Visualization of the docking results was conducted using molecular visualization tools such as Drug Discovery studio (BIOVIA), enabling the analysis of binding modes and interactions between flavonoids and their respective protein targets. This visualization aids in identifying key amino acid residues involved in ligand binding, as well as understanding the structural basis of Ligand-receptor interactions. The findings highlight specific flavonoids that exhibit favourable binding affinities and interactions with the targeted neurochemical receptors and enzymes. Such insights are crucial for guiding further experimental validation and optimization of flavonoid-based compounds as potential therapeutic agents for neurological...
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Posted by admin on Nov 30, 2024 in |
Background: Obsessive-Compulsive Disorder (OCD) is a chronic mental health condition that significantly impairs an individual’s quality of life (QOL), affecting physical, psychological, social, and environmental well-being. In India, particularly in Northern regions, OCD patients face unique socio-cultural and healthcare challenges, which may exacerbate the impact of the disorder. This study aims to assess the QOL outcomes of OCD patients receiving treatment at a tertiary care psychiatry outpatient department in Northern India. Methods: A prospective observational study was conducted at King George’s Medical University, Lucknow, involving 33 patients diagnosed with OCD, aged 18-60. The WHOQOL-BREF questionnaire was used to assess QOL across four domains: physical health, psychological health, social relationships, and environmental factors. Patients were followed up at 1, 3, and 6 months. Statistical analysis was performed using SPSS version 26, and significant differences were identified using ANOVA. Results: The study found significant improvements in specific domains of the WHOQOL-BREF, particularly in social functioning and psychological well-being, following treatment interventions. The results suggest that pharmacological and psychotherapeutic treatments contribute to...
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